Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
Nitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1164441/full |
| _version_ | 1797831667125059584 |
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| author | Fei Wang Yan Wang Luying Ying Hong Lu Yijian Liu Yu Liu Jiming Xu Yunrong Wu Xiaorong Mo Zhongchang Wu Chuanzao Mao Chuanzao Mao |
| author_facet | Fei Wang Yan Wang Luying Ying Hong Lu Yijian Liu Yu Liu Jiming Xu Yunrong Wu Xiaorong Mo Zhongchang Wu Chuanzao Mao Chuanzao Mao |
| author_sort | Fei Wang |
| collection | DOAJ |
| description | Nitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how N and P signaling pathways are integrated. We performed transcriptomic analyses and physiological experiments to explore gene expression profiles and physiological homeostasis in the response of rice (Oryza sativa) to N and P deficiency. We revealed that N and P shortage inhibit rice growth and uptake of other nutrients. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) suggested that N and Pi deficiency stimulate specific different physiological reactions and also some same physiological processes in rice. We established the transcriptional regulatory network between N and P signaling pathways based on all DEGs. We determined that the transcript levels of 763 core genes changed under both N or P starvation conditions. Among these core genes, we focused on the transcription factor gene NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) and show that its encoded protein is a positive regulator of P homeostasis and a negative regulator of N acquisition in rice. NIGT1 promoted Pi uptake but inhibited N absorption, induced the expression of Pi responsive genes PT2 and SPX1 and repressed the N responsive genes NLP1 and NRT2.1. These results provide new clues about the mechanisms underlying the interaction between plant N and P starvation responses. |
| first_indexed | 2024-04-09T13:56:33Z |
| format | Article |
| id | doaj.art-25e43f1456f945a297ebfa07f64e6812 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T13:56:33Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-25e43f1456f945a297ebfa07f64e68122023-05-08T04:42:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-05-011410.3389/fpls.2023.11644411164441Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in riceFei Wang0Yan Wang1Luying Ying2Hong Lu3Yijian Liu4Yu Liu5Jiming Xu6Yunrong Wu7Xiaorong Mo8Zhongchang Wu9Chuanzao Mao10Chuanzao Mao11State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaHainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Sanya, Hainan, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, ChinaHainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Sanya, Hainan, ChinaNitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how N and P signaling pathways are integrated. We performed transcriptomic analyses and physiological experiments to explore gene expression profiles and physiological homeostasis in the response of rice (Oryza sativa) to N and P deficiency. We revealed that N and P shortage inhibit rice growth and uptake of other nutrients. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) suggested that N and Pi deficiency stimulate specific different physiological reactions and also some same physiological processes in rice. We established the transcriptional regulatory network between N and P signaling pathways based on all DEGs. We determined that the transcript levels of 763 core genes changed under both N or P starvation conditions. Among these core genes, we focused on the transcription factor gene NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) and show that its encoded protein is a positive regulator of P homeostasis and a negative regulator of N acquisition in rice. NIGT1 promoted Pi uptake but inhibited N absorption, induced the expression of Pi responsive genes PT2 and SPX1 and repressed the N responsive genes NLP1 and NRT2.1. These results provide new clues about the mechanisms underlying the interaction between plant N and P starvation responses.https://www.frontiersin.org/articles/10.3389/fpls.2023.1164441/fullricenitrogenphosphateinteractionuptaketranscriptome |
| spellingShingle | Fei Wang Yan Wang Luying Ying Hong Lu Yijian Liu Yu Liu Jiming Xu Yunrong Wu Xiaorong Mo Zhongchang Wu Chuanzao Mao Chuanzao Mao Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice Frontiers in Plant Science rice nitrogen phosphate interaction uptake transcriptome |
| title | Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice |
| title_full | Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice |
| title_fullStr | Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice |
| title_full_unstemmed | Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice |
| title_short | Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice |
| title_sort | integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice |
| topic | rice nitrogen phosphate interaction uptake transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1164441/full |
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