Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2016-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605&lng=en&tlng=en |
| _version_ | 1818061920837566464 |
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| author | L. Zhang Y. Ding G.Z. Rao D. Miao |
| author_facet | L. Zhang Y. Ding G.Z. Rao D. Miao |
| author_sort | L. Zhang |
| collection | DOAJ |
| description | The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease. |
| first_indexed | 2024-12-10T13:55:59Z |
| format | Article |
| id | doaj.art-25e66d605ccf4f72a366ed4354835b28 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-10T13:55:59Z |
| publishDate | 2016-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-25e66d605ccf4f72a366ed4354835b282022-12-22T01:45:57ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2016-01-0149410.1590/1414-431X20154324S0100-879X2016000400605Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblastsL. ZhangY. DingG.Z. RaoD. MiaoThe effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605&lng=en&tlng=enHuman periodontal ligament fibroblastsInterleukin-10GlucoseOsteoprotegerinReceptor activator of nuclear factor-κB ligand |
| spellingShingle | L. Zhang Y. Ding G.Z. Rao D. Miao Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts Brazilian Journal of Medical and Biological Research Human periodontal ligament fibroblasts Interleukin-10 Glucose Osteoprotegerin Receptor activator of nuclear factor-κB ligand |
| title | Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts |
| title_full | Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts |
| title_fullStr | Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts |
| title_full_unstemmed | Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts |
| title_short | Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts |
| title_sort | effects of il 10 and glucose on expression of opg and rankl in human periodontal ligament fibroblasts |
| topic | Human periodontal ligament fibroblasts Interleukin-10 Glucose Osteoprotegerin Receptor activator of nuclear factor-κB ligand |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605&lng=en&tlng=en |
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