Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet
A layered construct was developed by combining a porous polymer sheet and a cell sheet as a tissue engineered vascular patch. The primary objective of this study is to investigate the influence of mesenchymal stem cells (MSCs) sheet on the tensile mechanical properties of porous poly-(l-lactide-co-ε...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-06-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | http://www.mdpi.com/2079-4983/7/2/14 |
| _version_ | 1798041775451930624 |
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| author | Azizah Intan Pangesty Takaaki Arahira Mitsugu Todo |
| author_facet | Azizah Intan Pangesty Takaaki Arahira Mitsugu Todo |
| author_sort | Azizah Intan Pangesty |
| collection | DOAJ |
| description | A layered construct was developed by combining a porous polymer sheet and a cell sheet as a tissue engineered vascular patch. The primary objective of this study is to investigate the influence of mesenchymal stem cells (MSCs) sheet on the tensile mechanical properties of porous poly-(l-lactide-co-ε-caprolactone) (PLCL) sheet. The porous PLCL sheet was fabricated by the solid-liquid phase separation method and the following freeze-drying method. The MSCs sheet, prepared by the temperature-responsive dish, was then layered on the top of the PLCL sheet and cultured for 2 weeks. During the in vitro study, cellular properties such as cell infiltration, spreading and proliferation were evaluated. Tensile test of the layered construct was performed periodically to characterize the tensile mechanical behavior. The tensile properties were then correlated with the cellular properties to understand the effect of MSCs sheet on the variation of the mechanical behavior during the in vitro study. It was found that MSCs from the cell sheet were able to migrate into the PLCL sheet and actively proliferated into the porous structure then formed a new layer of MSCs on the opposite surface of the PLCL sheet. Mechanical evaluation revealed that the PLCL sheet with MSCs showed enhancement of tensile strength and strain energy density at the first week of culture which is characterized as the effect of MSCs proliferation and its infiltration into the porous structure of the PLCL sheet. New technique was presented to develop tissue engineered patch by combining MSCs sheet and porous PLCL sheet, and it is expected that the layered patch may prolong biomechanical stability when implanted in vivo. |
| first_indexed | 2024-04-11T22:26:19Z |
| format | Article |
| id | doaj.art-25e75975ea514c2bb8b8e368dcaa6997 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-11T22:26:19Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-25e75975ea514c2bb8b8e368dcaa69972022-12-22T03:59:38ZengMDPI AGJournal of Functional Biomaterials2079-49832016-06-01721410.3390/jfb7020014jfb7020014Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered SheetAzizah Intan Pangesty0Takaaki Arahira1Mitsugu Todo2Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, Fukuoka 816-8580, JapanFukuoka Dental Collage, Fukuoka 814-0193, JapanResearch Institute for Applied Mechanics, Kyushu University, Fukuoka 816-8580, JapanA layered construct was developed by combining a porous polymer sheet and a cell sheet as a tissue engineered vascular patch. The primary objective of this study is to investigate the influence of mesenchymal stem cells (MSCs) sheet on the tensile mechanical properties of porous poly-(l-lactide-co-ε-caprolactone) (PLCL) sheet. The porous PLCL sheet was fabricated by the solid-liquid phase separation method and the following freeze-drying method. The MSCs sheet, prepared by the temperature-responsive dish, was then layered on the top of the PLCL sheet and cultured for 2 weeks. During the in vitro study, cellular properties such as cell infiltration, spreading and proliferation were evaluated. Tensile test of the layered construct was performed periodically to characterize the tensile mechanical behavior. The tensile properties were then correlated with the cellular properties to understand the effect of MSCs sheet on the variation of the mechanical behavior during the in vitro study. It was found that MSCs from the cell sheet were able to migrate into the PLCL sheet and actively proliferated into the porous structure then formed a new layer of MSCs on the opposite surface of the PLCL sheet. Mechanical evaluation revealed that the PLCL sheet with MSCs showed enhancement of tensile strength and strain energy density at the first week of culture which is characterized as the effect of MSCs proliferation and its infiltration into the porous structure of the PLCL sheet. New technique was presented to develop tissue engineered patch by combining MSCs sheet and porous PLCL sheet, and it is expected that the layered patch may prolong biomechanical stability when implanted in vivo.http://www.mdpi.com/2079-4983/7/2/14cell sheet technologyscaffold tissue engineeringPLCL sheetMSCs sheettensile mechanical propertiestissue engineered patch |
| spellingShingle | Azizah Intan Pangesty Takaaki Arahira Mitsugu Todo Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet Journal of Functional Biomaterials cell sheet technology scaffold tissue engineering PLCL sheet MSCs sheet tensile mechanical properties tissue engineered patch |
| title | Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet |
| title_full | Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet |
| title_fullStr | Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet |
| title_full_unstemmed | Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet |
| title_short | Characterization of Tensile Mechanical Behavior of MSCs/PLCL Hybrid Layered Sheet |
| title_sort | characterization of tensile mechanical behavior of mscs plcl hybrid layered sheet |
| topic | cell sheet technology scaffold tissue engineering PLCL sheet MSCs sheet tensile mechanical properties tissue engineered patch |
| url | http://www.mdpi.com/2079-4983/7/2/14 |
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