RNA-Guided <i>As</i>Cas12a- and <i>Sp</i>Cas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, <i>Schistosoma mansoni</i>

RNA-Guided <i>As</i>Cas12a- and <i>Sp</i>Cas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, <i>Schistosoma mansoni</i>

The efficiency of the RNA-guided <i>As</i>Cas12a nuclease of <i>Acidaminococcus</i> sp. was compared with <i>Sp</i>Cas9 from <i>Streptococcus pyogenes</i>, for functional genomics in <i>Schistosoma mansoni</i>. We deployed optimized conditi...

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Bibliographic Details
Main Authors: Wannaporn Ittiprasert, Chawalit Chatupheeraphat, Victoria H. Mann, Wenhui Li, André Miller, Taiwo Ogunbayo, Kenny Tran, Yousef N. Alrefaei, Margaret Mentink-Kane, Paul J. Brindley
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:International Journal of Molecular Sciences
Subjects:
<i>As</i>Cas12a
<i>Sp</i>Cas9
ribonucleoprotein complex
CRISPR
omega-1
genome editing
Online Access:https://www.mdpi.com/1422-0067/23/2/631

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