MXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturation
Abstract Electroconductive biomaterials are gaining significant consideration for regeneration in tissues where electrical functionality is of crucial importance, such as myocardium, neural, musculoskeletal, and bone tissue. In this work, conductive biohybrid platforms were engineered by blending co...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-06-01
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| Series: | npj 2D Materials and Applications |
| Online Access: | https://doi.org/10.1038/s41699-023-00409-w |
| _version_ | 1797789686796648448 |
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| author | Giuseppe A. Asaro Matteo Solazzo Meenakshi Suku Dahnan Spurling Katelyn Genoud Javier Gutierrez Gonzalez Fergal J. O’ Brien Valeria Nicolosi Michael G. Monaghan |
| author_facet | Giuseppe A. Asaro Matteo Solazzo Meenakshi Suku Dahnan Spurling Katelyn Genoud Javier Gutierrez Gonzalez Fergal J. O’ Brien Valeria Nicolosi Michael G. Monaghan |
| author_sort | Giuseppe A. Asaro |
| collection | DOAJ |
| description | Abstract Electroconductive biomaterials are gaining significant consideration for regeneration in tissues where electrical functionality is of crucial importance, such as myocardium, neural, musculoskeletal, and bone tissue. In this work, conductive biohybrid platforms were engineered by blending collagen type I and 2D MXene (Ti3C2Tx) and afterwards covalently crosslinking; to harness the biofunctionality of the protein component and the increased stiffness and enhanced electrical conductivity (matching and even surpassing native tissues) that two-dimensional titanium carbide provides. These MXene platforms were highly biocompatible and resulted in increased proliferation and cell spreading when seeded with fibroblasts. Conversely, they limited bacterial attachment (Staphylococcus aureus) and proliferation. When neonatal rat cardiomyocytes (nrCMs) were cultured on the substrates increased spreading and viability up to day 7 were studied when compared to control collagen substrates. Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) were seeded and stimulated using electric-field generation in a custom-made bioreactor. The combination of an electroconductive substrate with an external electrical field enhanced cell growth, and significantly increased cx43 expression. This in vitro study convincingly demonstrates the potential of this engineered conductive biohybrid platform for cardiac tissue regeneration. |
| first_indexed | 2024-03-13T01:54:13Z |
| format | Article |
| id | doaj.art-25e87bce6a9b4dba94504f53fb690d82 |
| institution | Directory Open Access Journal |
| issn | 2397-7132 |
| language | English |
| last_indexed | 2024-03-13T01:54:13Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj 2D Materials and Applications |
| spelling | doaj.art-25e87bce6a9b4dba94504f53fb690d822023-07-02T11:17:35ZengNature Portfolionpj 2D Materials and Applications2397-71322023-06-017111310.1038/s41699-023-00409-wMXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturationGiuseppe A. Asaro0Matteo Solazzo1Meenakshi Suku2Dahnan Spurling3Katelyn Genoud4Javier Gutierrez Gonzalez5Fergal J. O’ Brien6Valeria Nicolosi7Michael G. Monaghan8Department of Mechanical, Manufacturing and Biomedical Engineering, Trinity College DublinDepartment of Mechanical, Manufacturing and Biomedical Engineering, Trinity College DublinDepartment of Mechanical, Manufacturing and Biomedical Engineering, Trinity College DublinAdvanced Materials and BioEngineering Research (AMBER), Centre at Trinity College Dublin and the Royal College of Surgeons in IrelandAdvanced Materials and BioEngineering Research (AMBER), Centre at Trinity College Dublin and the Royal College of Surgeons in IrelandAdvanced Materials and BioEngineering Research (AMBER), Centre at Trinity College Dublin and the Royal College of Surgeons in IrelandAdvanced Materials and BioEngineering Research (AMBER), Centre at Trinity College Dublin and the Royal College of Surgeons in IrelandAdvanced Materials and BioEngineering Research (AMBER), Centre at Trinity College Dublin and the Royal College of Surgeons in IrelandDepartment of Mechanical, Manufacturing and Biomedical Engineering, Trinity College DublinAbstract Electroconductive biomaterials are gaining significant consideration for regeneration in tissues where electrical functionality is of crucial importance, such as myocardium, neural, musculoskeletal, and bone tissue. In this work, conductive biohybrid platforms were engineered by blending collagen type I and 2D MXene (Ti3C2Tx) and afterwards covalently crosslinking; to harness the biofunctionality of the protein component and the increased stiffness and enhanced electrical conductivity (matching and even surpassing native tissues) that two-dimensional titanium carbide provides. These MXene platforms were highly biocompatible and resulted in increased proliferation and cell spreading when seeded with fibroblasts. Conversely, they limited bacterial attachment (Staphylococcus aureus) and proliferation. When neonatal rat cardiomyocytes (nrCMs) were cultured on the substrates increased spreading and viability up to day 7 were studied when compared to control collagen substrates. Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) were seeded and stimulated using electric-field generation in a custom-made bioreactor. The combination of an electroconductive substrate with an external electrical field enhanced cell growth, and significantly increased cx43 expression. This in vitro study convincingly demonstrates the potential of this engineered conductive biohybrid platform for cardiac tissue regeneration.https://doi.org/10.1038/s41699-023-00409-w |
| spellingShingle | Giuseppe A. Asaro Matteo Solazzo Meenakshi Suku Dahnan Spurling Katelyn Genoud Javier Gutierrez Gonzalez Fergal J. O’ Brien Valeria Nicolosi Michael G. Monaghan MXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturation npj 2D Materials and Applications |
| title | MXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturation |
| title_full | MXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturation |
| title_fullStr | MXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturation |
| title_full_unstemmed | MXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturation |
| title_short | MXene functionalized collagen biomaterials for cardiac tissue engineering driving iPSC-derived cardiomyocyte maturation |
| title_sort | mxene functionalized collagen biomaterials for cardiac tissue engineering driving ipsc derived cardiomyocyte maturation |
| url | https://doi.org/10.1038/s41699-023-00409-w |
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