Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples.
In studies where [1-13C]palmitic acid is employed as isotopic tracer in vivo, we have described a selected ion monitoring gas-liquid chromatography-mass spectrometry micro-method which allows plasma tracer enrichment as well as plasma palmitate content to be determined in the same 100-microliter sam...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1982-03-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520381499 |
| _version_ | 1818459746271756288 |
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| author | P F Bougnères D M Bier |
| author_facet | P F Bougnères D M Bier |
| author_sort | P F Bougnères |
| collection | DOAJ |
| description | In studies where [1-13C]palmitic acid is employed as isotopic tracer in vivo, we have described a selected ion monitoring gas-liquid chromatography-mass spectrometry micro-method which allows plasma tracer enrichment as well as plasma palmitate content to be determined in the same 100-microliter sample through the use of [5,5,6,6-2H4]palmitic acid as assay internal standard. For standard solutions in the range equivalent to plasma palmitic acid concentrations of 10--2500 microM, assay precision was +/- 5%. For plasma samples in the physiologic range (approximately 30--200 microM palmitate) assay precision averaged better than +/- 2%. The use of the method is illustrated by measuring palmitate turnover in a 12-hr-old human infant. |
| first_indexed | 2024-12-14T23:19:15Z |
| format | Article |
| id | doaj.art-25e8aa31c6834597b3a5c2787c840bf7 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T23:19:15Z |
| publishDate | 1982-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-25e8aa31c6834597b3a5c2787c840bf72022-12-21T22:44:00ZengElsevierJournal of Lipid Research0022-22751982-03-01233502507Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples.P F BougnèresD M BierIn studies where [1-13C]palmitic acid is employed as isotopic tracer in vivo, we have described a selected ion monitoring gas-liquid chromatography-mass spectrometry micro-method which allows plasma tracer enrichment as well as plasma palmitate content to be determined in the same 100-microliter sample through the use of [5,5,6,6-2H4]palmitic acid as assay internal standard. For standard solutions in the range equivalent to plasma palmitic acid concentrations of 10--2500 microM, assay precision was +/- 5%. For plasma samples in the physiologic range (approximately 30--200 microM palmitate) assay precision averaged better than +/- 2%. The use of the method is illustrated by measuring palmitate turnover in a 12-hr-old human infant.http://www.sciencedirect.com/science/article/pii/S0022227520381499 |
| spellingShingle | P F Bougnères D M Bier Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples. Journal of Lipid Research |
| title | Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples. |
| title_full | Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples. |
| title_fullStr | Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples. |
| title_full_unstemmed | Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples. |
| title_short | Stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples. |
| title_sort | stable isotope dilution method for measurement of palmitate content and labeled palmitate tracer enrichment in microliter plasma samples |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520381499 |
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