Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23
Abstract Background Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocercia...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3308-z |
| _version_ | 1819119831402676224 |
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| author | Johnny Vlaminck Ole Lagatie Ann Verheyen Daniel Dana Bieke Van Dorst Zeleke Mekonnen Bruno Levecke Lieven J. Stuyver |
| author_facet | Johnny Vlaminck Ole Lagatie Ann Verheyen Daniel Dana Bieke Van Dorst Zeleke Mekonnen Bruno Levecke Lieven J. Stuyver |
| author_sort | Johnny Vlaminck |
| collection | DOAJ |
| description | Abstract Background Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. Recently, two linear epitopes encoded in OvMP-1 and OvMP-23 peptides were introduced as serological markers, but the observed antibody cross-reactivity in samples originating from Onchocerca volvulus non-endemic areas required further investigation. Methods We evaluated both peptide markers in an O. volvulus hypo-endemic setting in Jimma Town, Ethiopia using peptide ELISA. For all individuals (n = 303), the infection status with soil-transmitted helminths and Schistosoma mansoni was known. Results We found that 11 (3.6%) individuals were positive for anti-Ov16 IgG4 antibodies, while 34 (11.2%) and 15 (5.0%) individuals were positive for OvMP-1 and OvMP-23, respectively. Out of the 34 OvMP-1 positive samples, 33 were negative on the Ov16 IgG4 ELISA. Similarly, out of the 15 OvMP-23 positive samples, 14 scored negative on this reference method. No difference in seroprevalence for all three markers could be observed between uninfected individuals and individuals infected with different soil-transmitted helminths or S. mansoni. Moreover, the intensity of the response to OvMP-1, OvMP-23 or Ov16 was not significantly stronger in individuals carrying patent STH or S. mansoni infections, nor was there any correlation between the intensities of the responses to the three different antigens. Conclusions This study demonstrates that a patent infection with either soil-transmitted helminths or S. mansoni does not lead to increased antibody recognition of both OvMP-1 and OvMP23. |
| first_indexed | 2024-12-22T06:11:01Z |
| format | Article |
| id | doaj.art-25eadaafd824415d8f88ec304f6ec52f |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-22T06:11:01Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-25eadaafd824415d8f88ec304f6ec52f2022-12-21T18:36:14ZengBMCParasites & Vectors1756-33052019-01-011211710.1186/s13071-019-3308-zPatent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23Johnny Vlaminck0Ole Lagatie1Ann Verheyen2Daniel Dana3Bieke Van Dorst4Zeleke Mekonnen5Bruno Levecke6Lieven J. Stuyver7Department of Virology, Parasitology and Immunology, University of GhentJanssen Diagnostics, Janssen R&DJanssen Diagnostics, Janssen R&DSchool of Medical Laboratory Sciences, Jimma UniversityJanssen Diagnostics, Janssen R&DSchool of Medical Laboratory Sciences, Jimma UniversityDepartment of Virology, Parasitology and Immunology, University of GhentJanssen Diagnostics, Janssen R&DAbstract Background Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. Recently, two linear epitopes encoded in OvMP-1 and OvMP-23 peptides were introduced as serological markers, but the observed antibody cross-reactivity in samples originating from Onchocerca volvulus non-endemic areas required further investigation. Methods We evaluated both peptide markers in an O. volvulus hypo-endemic setting in Jimma Town, Ethiopia using peptide ELISA. For all individuals (n = 303), the infection status with soil-transmitted helminths and Schistosoma mansoni was known. Results We found that 11 (3.6%) individuals were positive for anti-Ov16 IgG4 antibodies, while 34 (11.2%) and 15 (5.0%) individuals were positive for OvMP-1 and OvMP-23, respectively. Out of the 34 OvMP-1 positive samples, 33 were negative on the Ov16 IgG4 ELISA. Similarly, out of the 15 OvMP-23 positive samples, 14 scored negative on this reference method. No difference in seroprevalence for all three markers could be observed between uninfected individuals and individuals infected with different soil-transmitted helminths or S. mansoni. Moreover, the intensity of the response to OvMP-1, OvMP-23 or Ov16 was not significantly stronger in individuals carrying patent STH or S. mansoni infections, nor was there any correlation between the intensities of the responses to the three different antigens. Conclusions This study demonstrates that a patent infection with either soil-transmitted helminths or S. mansoni does not lead to increased antibody recognition of both OvMP-1 and OvMP23.http://link.springer.com/article/10.1186/s13071-019-3308-zOnchocerca volvulusRiver blindnessOnchocerciasisSerologyLinear epitopeSoil-transmitted helminths |
| spellingShingle | Johnny Vlaminck Ole Lagatie Ann Verheyen Daniel Dana Bieke Van Dorst Zeleke Mekonnen Bruno Levecke Lieven J. Stuyver Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23 Parasites & Vectors Onchocerca volvulus River blindness Onchocerciasis Serology Linear epitope Soil-transmitted helminths |
| title | Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23 |
| title_full | Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23 |
| title_fullStr | Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23 |
| title_full_unstemmed | Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23 |
| title_short | Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23 |
| title_sort | patent infections with soil transmitted helminths and schistosoma mansoni are not associated with increased prevalence of antibodies to the onchocerca volvulus peptide epitopes ovmp 1 and ovmp 23 |
| topic | Onchocerca volvulus River blindness Onchocerciasis Serology Linear epitope Soil-transmitted helminths |
| url | http://link.springer.com/article/10.1186/s13071-019-3308-z |
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