DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese
Skeletal muscle development from embryonic stages to hatching is critical for poultry muscle growth, during which DNA methylation plays a vital role. However, it is not yet clear how DNA methylation affects early embryonic muscle development between goose breeds of different body size. In this study...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/8/7188 |
| _version_ | 1797605133463322624 |
|---|---|
| author | Xumeng Zhang Yong Li Chenyu Zhu Fada Li Zhiyuan Liu Xiujin Li Xu Shen Zhongping Wu Mengsi Fu Danning Xu Yunbo Tian Yunmao Huang |
| author_facet | Xumeng Zhang Yong Li Chenyu Zhu Fada Li Zhiyuan Liu Xiujin Li Xu Shen Zhongping Wu Mengsi Fu Danning Xu Yunbo Tian Yunmao Huang |
| author_sort | Xumeng Zhang |
| collection | DOAJ |
| description | Skeletal muscle development from embryonic stages to hatching is critical for poultry muscle growth, during which DNA methylation plays a vital role. However, it is not yet clear how DNA methylation affects early embryonic muscle development between goose breeds of different body size. In this study, whole genome bisulfite sequencing (WGBS) was conducted on leg muscle tissue from Wuzong (WZE) and Shitou (STE) geese on embryonic day 15 (E15), E23, and post-hatch day 1. It was found that at E23, the embryonic leg muscle development of STE was more intense than that of WZE. A negative correlation was found between gene expression and DNA methylation around transcription start sites (TSSs), while a positive correlation was observed in the gene body near TTSs. It was also possible that earlier demethylation of myogenic genes around TSSs contributes to their earlier expression in WZE. Using pyrosequencing to analyze DNA methylation patterns of promoter regions, we also found that earlier demethylation of the <i>MyoD1</i> promoter in WZE contributed to its earlier expression. This study reveals that DNA demethylation of myogenic genes may contribute to embryonic leg muscle development differences between Wuzong and Shitou geese. |
| first_indexed | 2024-03-11T04:56:50Z |
| format | Article |
| id | doaj.art-25f2a1eda2ac4504b98cf51d057256d1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:56:50Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-25f2a1eda2ac4504b98cf51d057256d12023-11-17T19:36:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248718810.3390/ijms24087188DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou GeeseXumeng Zhang0Yong Li1Chenyu Zhu2Fada Li3Zhiyuan Liu4Xiujin Li5Xu Shen6Zhongping Wu7Mengsi Fu8Danning Xu9Yunbo Tian10Yunmao Huang11College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaSkeletal muscle development from embryonic stages to hatching is critical for poultry muscle growth, during which DNA methylation plays a vital role. However, it is not yet clear how DNA methylation affects early embryonic muscle development between goose breeds of different body size. In this study, whole genome bisulfite sequencing (WGBS) was conducted on leg muscle tissue from Wuzong (WZE) and Shitou (STE) geese on embryonic day 15 (E15), E23, and post-hatch day 1. It was found that at E23, the embryonic leg muscle development of STE was more intense than that of WZE. A negative correlation was found between gene expression and DNA methylation around transcription start sites (TSSs), while a positive correlation was observed in the gene body near TTSs. It was also possible that earlier demethylation of myogenic genes around TSSs contributes to their earlier expression in WZE. Using pyrosequencing to analyze DNA methylation patterns of promoter regions, we also found that earlier demethylation of the <i>MyoD1</i> promoter in WZE contributed to its earlier expression. This study reveals that DNA demethylation of myogenic genes may contribute to embryonic leg muscle development differences between Wuzong and Shitou geese.https://www.mdpi.com/1422-0067/24/8/7188DNA methylationWGBSgooseembryonic myogenesis<i>MyoD1</i> |
| spellingShingle | Xumeng Zhang Yong Li Chenyu Zhu Fada Li Zhiyuan Liu Xiujin Li Xu Shen Zhongping Wu Mengsi Fu Danning Xu Yunbo Tian Yunmao Huang DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese International Journal of Molecular Sciences DNA methylation WGBS goose embryonic myogenesis <i>MyoD1</i> |
| title | DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese |
| title_full | DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese |
| title_fullStr | DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese |
| title_full_unstemmed | DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese |
| title_short | DNA Demethylation of Myogenic Genes May Contribute to Embryonic Leg Muscle Development Differences between Wuzong and Shitou Geese |
| title_sort | dna demethylation of myogenic genes may contribute to embryonic leg muscle development differences between wuzong and shitou geese |
| topic | DNA methylation WGBS goose embryonic myogenesis <i>MyoD1</i> |
| url | https://www.mdpi.com/1422-0067/24/8/7188 |
| work_keys_str_mv | AT xumengzhang dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT yongli dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT chenyuzhu dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT fadali dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT zhiyuanliu dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT xiujinli dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT xushen dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT zhongpingwu dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT mengsifu dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT danningxu dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT yunbotian dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese AT yunmaohuang dnademethylationofmyogenicgenesmaycontributetoembryoniclegmuscledevelopmentdifferencesbetweenwuzongandshitougeese |