A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media
The present study assessed the fertilization potential of sperm obtained from refrigerated epididymis and fresh ejaculation of buck under 2 culture media, viz. modified synthetic oviductal fluid (mSOF) and research vitro cleave-blastocyst (RVCL-BLAST) media. In the process, the cauda epididymis of...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2016-08-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/60778 |
| _version_ | 1797745033610264576 |
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| author | DHARMENDRA KUMAR SALIL KUMAR JAIN BIKASH CHANDRA SARKHEL |
| author_facet | DHARMENDRA KUMAR SALIL KUMAR JAIN BIKASH CHANDRA SARKHEL |
| author_sort | DHARMENDRA KUMAR |
| collection | DOAJ |
| description |
The present study assessed the fertilization potential of sperm obtained from refrigerated epididymis and fresh ejaculation of buck under 2 culture media, viz. modified synthetic oviductal fluid (mSOF) and research vitro cleave-blastocyst (RVCL-BLAST) media. In the process, the cauda epididymis of slaughtered buck were immediately collected, pre-processed and refrigerated (4°C) for 24 h. The oocytes recovered from slaughter house ovaries were in vitro matured and fertilized with both semen sources. On analysis of embryonic development, ejaculated group showed significantly higher blastocyst rate than epididymal sperm, however, initial cleavage rates of epididymal group was significantly higher than ejaculated semen, irrespective of culture media. Among the culture media, RVCL-BLAST media was significantly efficient than mSOF in terms of cleavage and blastocysts, irrespective of gametes sources. Despite of lower outcome of blastocyst, cold stored epididymal sperm may be considered as an alternative, potent and viable source of gametes for assisted reproduction in case of endangered or other livestock, if immediate facilities for sperm processing are not available.
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| first_indexed | 2024-03-12T15:17:42Z |
| format | Article |
| id | doaj.art-25f513c46725490a969ff5bdfc74bba3 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-12T15:17:42Z |
| publishDate | 2016-08-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-25f513c46725490a969ff5bdfc74bba32023-08-11T10:19:41ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272016-08-0186810.56093/ijans.v86i8.60778A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture mediaDHARMENDRA KUMAR0SALIL KUMAR JAIN1BIKASH CHANDRA SARKHEL2Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 IndiaNanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 IndiaNanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India The present study assessed the fertilization potential of sperm obtained from refrigerated epididymis and fresh ejaculation of buck under 2 culture media, viz. modified synthetic oviductal fluid (mSOF) and research vitro cleave-blastocyst (RVCL-BLAST) media. In the process, the cauda epididymis of slaughtered buck were immediately collected, pre-processed and refrigerated (4°C) for 24 h. The oocytes recovered from slaughter house ovaries were in vitro matured and fertilized with both semen sources. On analysis of embryonic development, ejaculated group showed significantly higher blastocyst rate than epididymal sperm, however, initial cleavage rates of epididymal group was significantly higher than ejaculated semen, irrespective of culture media. Among the culture media, RVCL-BLAST media was significantly efficient than mSOF in terms of cleavage and blastocysts, irrespective of gametes sources. Despite of lower outcome of blastocyst, cold stored epididymal sperm may be considered as an alternative, potent and viable source of gametes for assisted reproduction in case of endangered or other livestock, if immediate facilities for sperm processing are not available. https://epubs.icar.org.in/index.php/IJAnS/article/view/60778Culture mediumEpididymisGoatIn vitro fertilizationSemen |
| spellingShingle | DHARMENDRA KUMAR SALIL KUMAR JAIN BIKASH CHANDRA SARKHEL A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media Indian Journal of Animal Sciences Culture medium Epididymis Goat In vitro fertilization Semen |
| title | A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media |
| title_full | A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media |
| title_fullStr | A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media |
| title_full_unstemmed | A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media |
| title_short | A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media |
| title_sort | comparison of in vitro fertilization potential of sperm obtained from refrigerated 4°c epididymis and fresh ejaculation of buck under different embryo culture media |
| topic | Culture medium Epididymis Goat In vitro fertilization Semen |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/60778 |
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