Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review
From the early precipitation-based techniques, introduced more than a century ago, to the latest development of enzymatic bio- and nano-sensor applications, the analysis of phytic acid and/or other inositol phosphates has never been a straightforward analytical task. Due to the biomedical importance...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/1/174 |
| _version_ | 1797542723084877824 |
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| author | Gregor Marolt Mitja Kolar |
| author_facet | Gregor Marolt Mitja Kolar |
| author_sort | Gregor Marolt |
| collection | DOAJ |
| description | From the early precipitation-based techniques, introduced more than a century ago, to the latest development of enzymatic bio- and nano-sensor applications, the analysis of phytic acid and/or other inositol phosphates has never been a straightforward analytical task. Due to the biomedical importance, such as antinutritional, antioxidant and anticancer effects, several types of methodologies were investigated over the years to develop a reliable determination of these intriguing analytes in many types of biological samples; from various foodstuffs to living cell organisms. The main aim of the present work was to critically overview the development of the most relevant analytical principles, separation and detection methods that have been applied in order to overcome the difficulties with specific chemical properties of inositol phosphates, their interferences, absence of characteristic signal (e.g., absorbance), and strong binding interactions with (multivalent) metals and other biological molecules present in the sample matrix. A systematical and chronological review of the applied methodology and the detection system is given, ranging from the very beginnings of the classical gravimetric and titrimetric analysis, through the potentiometric titrations, chromatographic and electrophoretic separation techniques, to the use of spectroscopic methods and of the recently reported fluorescence and voltammetric bio- and nano-sensors. |
| first_indexed | 2024-03-10T13:34:37Z |
| format | Article |
| id | doaj.art-25f9f01c85544b08a445aba3d4b2d36f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:34:37Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-25f9f01c85544b08a445aba3d4b2d36f2023-11-21T07:37:47ZengMDPI AGMolecules1420-30492020-12-0126117410.3390/molecules26010174Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A ReviewGregor Marolt0Mitja Kolar1Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, SloveniaFaculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, SloveniaFrom the early precipitation-based techniques, introduced more than a century ago, to the latest development of enzymatic bio- and nano-sensor applications, the analysis of phytic acid and/or other inositol phosphates has never been a straightforward analytical task. Due to the biomedical importance, such as antinutritional, antioxidant and anticancer effects, several types of methodologies were investigated over the years to develop a reliable determination of these intriguing analytes in many types of biological samples; from various foodstuffs to living cell organisms. The main aim of the present work was to critically overview the development of the most relevant analytical principles, separation and detection methods that have been applied in order to overcome the difficulties with specific chemical properties of inositol phosphates, their interferences, absence of characteristic signal (e.g., absorbance), and strong binding interactions with (multivalent) metals and other biological molecules present in the sample matrix. A systematical and chronological review of the applied methodology and the detection system is given, ranging from the very beginnings of the classical gravimetric and titrimetric analysis, through the potentiometric titrations, chromatographic and electrophoretic separation techniques, to the use of spectroscopic methods and of the recently reported fluorescence and voltammetric bio- and nano-sensors.https://www.mdpi.com/1420-3049/26/1/174phytic acidinositol hexaphosphateinositol phosphatesanalytical methodspotentiometric titrationsion-exchange chromatography |
| spellingShingle | Gregor Marolt Mitja Kolar Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review Molecules phytic acid inositol hexaphosphate inositol phosphates analytical methods potentiometric titrations ion-exchange chromatography |
| title | Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review |
| title_full | Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review |
| title_fullStr | Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review |
| title_full_unstemmed | Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review |
| title_short | Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review |
| title_sort | analytical methods for determination of phytic acid and other inositol phosphates a review |
| topic | phytic acid inositol hexaphosphate inositol phosphates analytical methods potentiometric titrations ion-exchange chromatography |
| url | https://www.mdpi.com/1420-3049/26/1/174 |
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