The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content>
ABSTRACT Basic leucine zipper (bZIP) transcription factors are widespread in eukaryotes, including plants, animals, fungi, and oomycetes. However, the functions of bZIPs in oomycetes are rarely known. In this study, we identified a bZIP protein possessing a special bZIP-PAS structure in Peronophytho...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2020-06-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00261-20 |
| _version_ | 1818900594063048704 |
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| author | Guanghui Kong Yubin Chen Yizhen Deng Dinan Feng Liqun Jiang Lang Wan Minhui Li Zide Jiang Pinggen Xi |
| author_facet | Guanghui Kong Yubin Chen Yizhen Deng Dinan Feng Liqun Jiang Lang Wan Minhui Li Zide Jiang Pinggen Xi |
| author_sort | Guanghui Kong |
| collection | DOAJ |
| description | ABSTRACT Basic leucine zipper (bZIP) transcription factors are widespread in eukaryotes, including plants, animals, fungi, and oomycetes. However, the functions of bZIPs in oomycetes are rarely known. In this study, we identified a bZIP protein possessing a special bZIP-PAS structure in Peronophythora litchii, named PlBZP32. We found that PlBZP32 is upregulated in zoospores, in cysts, and during invasive hyphal growth. We studied the functions of PlBZP32 using the RNAi technique to suppress the expression of this gene. PlBZP32-silenced mutants were more sensitive to oxidative stress, showed a lower cyst germination rate, and produced more sporangia than the wild-type strain SHS3. The PlBZP32-silenced mutants were also less invasive on the host plant. Furthermore, we analyzed the activities of extracellular peroxidases and laccases and found that silencing PlBZP32 decreased the activities of P. litchii peroxidase and laccase. To our knowledge, this is the first report that the functions of a bZIP-PAS protein are associated with oxidative stress, asexual development, and pathogenicity in oomycetes. IMPORTANCE In this study, we utilized the RNAi technique to investigate the functions of PlBZP32, which possesses a basic leucine zipper (bZIP)-PAS structure, and provided insights into the contributions of bZIP transcription factors to oxidative stress, the production of sporangia, the germination of cysts, and the pathogenicity of Peronophythora litchii. This study also revealed the role of PlBZP32 in regulating the enzymatic activities of extracellular peroxidases and laccases in the plant-pathogenic oomycete. |
| first_indexed | 2024-12-19T20:06:20Z |
| format | Article |
| id | doaj.art-25fa72ccc0484e6eae9ac0250877956f |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-19T20:06:20Z |
| publishDate | 2020-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-25fa72ccc0484e6eae9ac0250877956f2022-12-21T20:07:27ZengAmerican Society for MicrobiologymSphere2379-50422020-06-015310.1128/mSphere.00261-20The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content>Guanghui Kong0Yubin Chen1Yizhen Deng2Dinan Feng3Liqun Jiang4Lang Wan5Minhui Li6Zide Jiang7Pinggen Xi8Department of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaABSTRACT Basic leucine zipper (bZIP) transcription factors are widespread in eukaryotes, including plants, animals, fungi, and oomycetes. However, the functions of bZIPs in oomycetes are rarely known. In this study, we identified a bZIP protein possessing a special bZIP-PAS structure in Peronophythora litchii, named PlBZP32. We found that PlBZP32 is upregulated in zoospores, in cysts, and during invasive hyphal growth. We studied the functions of PlBZP32 using the RNAi technique to suppress the expression of this gene. PlBZP32-silenced mutants were more sensitive to oxidative stress, showed a lower cyst germination rate, and produced more sporangia than the wild-type strain SHS3. The PlBZP32-silenced mutants were also less invasive on the host plant. Furthermore, we analyzed the activities of extracellular peroxidases and laccases and found that silencing PlBZP32 decreased the activities of P. litchii peroxidase and laccase. To our knowledge, this is the first report that the functions of a bZIP-PAS protein are associated with oxidative stress, asexual development, and pathogenicity in oomycetes. IMPORTANCE In this study, we utilized the RNAi technique to investigate the functions of PlBZP32, which possesses a basic leucine zipper (bZIP)-PAS structure, and provided insights into the contributions of bZIP transcription factors to oxidative stress, the production of sporangia, the germination of cysts, and the pathogenicity of Peronophythora litchii. This study also revealed the role of PlBZP32 in regulating the enzymatic activities of extracellular peroxidases and laccases in the plant-pathogenic oomycete.https://journals.asm.org/doi/10.1128/mSphere.00261-20Peronophythora litchiibZIP transcription factorpathogenicityperoxidaselaccase |
| spellingShingle | Guanghui Kong Yubin Chen Yizhen Deng Dinan Feng Liqun Jiang Lang Wan Minhui Li Zide Jiang Pinggen Xi The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> mSphere Peronophythora litchii bZIP transcription factor pathogenicity peroxidase laccase |
| title | The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_full | The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_fullStr | The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_full_unstemmed | The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_short | The Basic Leucine Zipper Transcription Factor PlBZP32 Associated with the Oxidative Stress Response Is Critical for Pathogenicity of the Lychee Downy Blight Oomycete <named-content content-type="genus-species">Peronophythora litchii</named-content> |
| title_sort | basic leucine zipper transcription factor plbzp32 associated with the oxidative stress response is critical for pathogenicity of the lychee downy blight oomycete named content content type genus species peronophythora litchii named content |
| topic | Peronophythora litchii bZIP transcription factor pathogenicity peroxidase laccase |
| url | https://journals.asm.org/doi/10.1128/mSphere.00261-20 |
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