<i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging Pathogens
This work aimed to determine the presence of bacterial pathogens in fish with a clinical picture suggestive of infectious disease in Nile tilapia reared in Chiapas, Mexico. Blood and viscera samples were taken from healthy and diseased animals from commercial farms. Clinical and pathological examina...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/23/3715 |
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| author | David Rajme-Manzur Jorge Hernández-López Marcel Martínez-Porchas Francisco Vargas-Albores Estefanía Garibay-Valdez Daniel Eduardo Coronado-Molina Miguel Ángel Hernández-Oñate Francisco Vázquez-Ramírez Luis Alfonso Velázquez-Valencia Azucena Santacruz |
| author_facet | David Rajme-Manzur Jorge Hernández-López Marcel Martínez-Porchas Francisco Vargas-Albores Estefanía Garibay-Valdez Daniel Eduardo Coronado-Molina Miguel Ángel Hernández-Oñate Francisco Vázquez-Ramírez Luis Alfonso Velázquez-Valencia Azucena Santacruz |
| author_sort | David Rajme-Manzur |
| collection | DOAJ |
| description | This work aimed to determine the presence of bacterial pathogens in fish with a clinical picture suggestive of infectious disease in Nile tilapia reared in Chiapas, Mexico. Blood and viscera samples were taken from healthy and diseased animals from commercial farms. Clinical and pathological examinations of each individual were performed and samples were collected for bacteriological studies. The bacterial isolates were identified and characterized by culture, biochemical tests, antibiogram, challenge tests and 16S rRNA sequencing. <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> were isolated from various diseased organisms. The clinical picture caused by <i>Staphylococcus haemolyticus</i> was characterized by appetite disorders, neurological signs, nodulation or ulceration in different areas and congestion or enlargement of internal organs. Providenciosis in juvenile specimens caused a characteristic picture of hemorrhagic septicemia. Challenge tests performed in healthy organisms revealed that both infections caused higher mortality rates in fish (<i>p</i> < 0.05) compared with non-infected specimens, with 100% survival. There was 100% mortality for animals infected with <i>P. vermicola</i> after three days post infection and 45% for those infected with <i>S. haemolyticus</i>. The isolation and identification of two pathogens involved in an infection process were achieved and cataloged as potential causal agents of disease outbreaks in tilapia farming in Mexico. This is the first report of possible bacterial infection caused by <i>S. haemolyticus</i> and <i>P. vermicola</i> in tilapia farms, which are two uncommon but potentially emerging pathogens for the species. |
| first_indexed | 2024-03-09T01:55:29Z |
| format | Article |
| id | doaj.art-25fd1d74bed044d192c042dbf56a9787 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T01:55:29Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-25fd1d74bed044d192c042dbf56a97872023-12-08T15:10:48ZengMDPI AGAnimals2076-26152023-11-011323371510.3390/ani13233715<i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging PathogensDavid Rajme-Manzur0Jorge Hernández-López1Marcel Martínez-Porchas2Francisco Vargas-Albores3Estefanía Garibay-Valdez4Daniel Eduardo Coronado-Molina5Miguel Ángel Hernández-Oñate6Francisco Vázquez-Ramírez7Luis Alfonso Velázquez-Valencia8Azucena Santacruz9Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia y Tecnología de Alimentos de Origen Animal, Biology of Aquatic Organisms, Hermosillo 83304, Sonora, MexicoCentro de Investigaciones Biológicas del Noroeste S. C. (CIBNOR), Unidad Hermosillo, Hermosillo 83106, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia y Tecnología de Alimentos de Origen Animal, Biology of Aquatic Organisms, Hermosillo 83304, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia y Tecnología de Alimentos de Origen Animal, Biology of Aquatic Organisms, Hermosillo 83304, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia y Tecnología de Alimentos de Origen Animal, Biology of Aquatic Organisms, Hermosillo 83304, Sonora, MexicoCentro de Investigaciones Biológicas del Noroeste S. C. (CIBNOR), Unidad Hermosillo, Hermosillo 83106, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia y Tecnología de Alimentos de Origen Animal, Biology of Aquatic Organisms, Hermosillo 83304, Sonora, MexicoComité Estatal de Sanidad Acuícola de Chiapas A.C., Tuxtla Gutiérrez 29020, Chiapas, MexicoComité Estatal de Sanidad Acuícola de Chiapas A.C., Tuxtla Gutiérrez 29020, Chiapas, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia y Tecnología de Alimentos de Origen Animal, Biology of Aquatic Organisms, Hermosillo 83304, Sonora, MexicoThis work aimed to determine the presence of bacterial pathogens in fish with a clinical picture suggestive of infectious disease in Nile tilapia reared in Chiapas, Mexico. Blood and viscera samples were taken from healthy and diseased animals from commercial farms. Clinical and pathological examinations of each individual were performed and samples were collected for bacteriological studies. The bacterial isolates were identified and characterized by culture, biochemical tests, antibiogram, challenge tests and 16S rRNA sequencing. <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> were isolated from various diseased organisms. The clinical picture caused by <i>Staphylococcus haemolyticus</i> was characterized by appetite disorders, neurological signs, nodulation or ulceration in different areas and congestion or enlargement of internal organs. Providenciosis in juvenile specimens caused a characteristic picture of hemorrhagic septicemia. Challenge tests performed in healthy organisms revealed that both infections caused higher mortality rates in fish (<i>p</i> < 0.05) compared with non-infected specimens, with 100% survival. There was 100% mortality for animals infected with <i>P. vermicola</i> after three days post infection and 45% for those infected with <i>S. haemolyticus</i>. The isolation and identification of two pathogens involved in an infection process were achieved and cataloged as potential causal agents of disease outbreaks in tilapia farming in Mexico. This is the first report of possible bacterial infection caused by <i>S. haemolyticus</i> and <i>P. vermicola</i> in tilapia farms, which are two uncommon but potentially emerging pathogens for the species.https://www.mdpi.com/2076-2615/13/23/3715Nile tilapiainfectious diseases<i>Staphylococcus</i><i>Providenciosis</i>fish disease |
| spellingShingle | David Rajme-Manzur Jorge Hernández-López Marcel Martínez-Porchas Francisco Vargas-Albores Estefanía Garibay-Valdez Daniel Eduardo Coronado-Molina Miguel Ángel Hernández-Oñate Francisco Vázquez-Ramírez Luis Alfonso Velázquez-Valencia Azucena Santacruz <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging Pathogens Animals Nile tilapia infectious diseases <i>Staphylococcus</i> <i>Providenciosis</i> fish disease |
| title | <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging Pathogens |
| title_full | <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging Pathogens |
| title_fullStr | <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging Pathogens |
| title_full_unstemmed | <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging Pathogens |
| title_short | <i>Staphylococcus haemolyticus</i> and <i>Providencia vermicola</i> Infections Occurring in Farmed Tilapia: Two Potentially Emerging Pathogens |
| title_sort | i staphylococcus haemolyticus i and i providencia vermicola i infections occurring in farmed tilapia two potentially emerging pathogens |
| topic | Nile tilapia infectious diseases <i>Staphylococcus</i> <i>Providenciosis</i> fish disease |
| url | https://www.mdpi.com/2076-2615/13/23/3715 |
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