Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes
<p>Abstract</p> <p>Background</p> <p>Type I interferons (IFNs) exhibit direct antiviral effects, but also distinct immunomodulatory properties. In this study, we analyzed type I IFN subtypes for their effect on prophylactic adenovirus-based anti-retroviral vaccination o...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | Retrovirology |
| Subjects: | |
| Online Access: | http://www.retrovirology.com/content/8/1/75 |
| _version_ | 1818344355739467776 |
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| author | Groitl Peter Schimmer Simone Nabi Ghulam Tenbusch Matthias Johrden Lena Ontikatze Teona Lietz Ruth Bayer Wibke Wolf Hans Berry Cassandra M Überla Klaus Dittmer Ulf Wildner Oliver |
| author_facet | Groitl Peter Schimmer Simone Nabi Ghulam Tenbusch Matthias Johrden Lena Ontikatze Teona Lietz Ruth Bayer Wibke Wolf Hans Berry Cassandra M Überla Klaus Dittmer Ulf Wildner Oliver |
| author_sort | Groitl Peter |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Type I interferons (IFNs) exhibit direct antiviral effects, but also distinct immunomodulatory properties. In this study, we analyzed type I IFN subtypes for their effect on prophylactic adenovirus-based anti-retroviral vaccination of mice against Friend retrovirus (FV) or HIV.</p> <p>Results</p> <p>Mice were vaccinated with adenoviral vectors encoding FV Env and Gag proteins alone or in combination with vectors encoding IFNα1, IFNα2, IFNα4, IFNα5, IFNα6, IFNα9 or IFNβ. Only the co-administration of adenoviral vectors encoding IFNα2, IFNα4, IFNα6 and IFNα9 resulted in strongly improved immune protection of vaccinated mice from subsequent FV challenge infection with high control over FV-induced splenomegaly and reduced viral loads. The level of protection correlated with augmented virus-specific CD4<sup>+ </sup>T cell responses and enhanced antibody titers. Similar results were obtained when mice were vaccinated against HIV with adenoviral vectors encoding HIV Env and Gag-Pol in combination with various type I IFN encoding vectors. Here mainly CD4<sup>+ </sup>T cell responses were enhanced by IFNα subtypes.</p> <p>Conclusions</p> <p>Our results indicate that certain IFNα subtypes have the potential to improve the protective effect of adenovirus-based vaccines against retroviruses. This correlated with augmented virus-specific CD4<sup>+ </sup>T cell and antibody responses. Thus, co-expression of select type I IFNs may be a valuable tool for the development of anti-retroviral vaccines.</p> |
| first_indexed | 2024-12-13T16:45:10Z |
| format | Article |
| id | doaj.art-25fe45015aad44b592d2757216e828aa |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-13T16:45:10Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-25fe45015aad44b592d2757216e828aa2022-12-21T23:38:10ZengBMCRetrovirology1742-46902011-09-01817510.1186/1742-4690-8-75Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypesGroitl PeterSchimmer SimoneNabi GhulamTenbusch MatthiasJohrden LenaOntikatze TeonaLietz RuthBayer WibkeWolf HansBerry Cassandra MÜberla KlausDittmer UlfWildner Oliver<p>Abstract</p> <p>Background</p> <p>Type I interferons (IFNs) exhibit direct antiviral effects, but also distinct immunomodulatory properties. In this study, we analyzed type I IFN subtypes for their effect on prophylactic adenovirus-based anti-retroviral vaccination of mice against Friend retrovirus (FV) or HIV.</p> <p>Results</p> <p>Mice were vaccinated with adenoviral vectors encoding FV Env and Gag proteins alone or in combination with vectors encoding IFNα1, IFNα2, IFNα4, IFNα5, IFNα6, IFNα9 or IFNβ. Only the co-administration of adenoviral vectors encoding IFNα2, IFNα4, IFNα6 and IFNα9 resulted in strongly improved immune protection of vaccinated mice from subsequent FV challenge infection with high control over FV-induced splenomegaly and reduced viral loads. The level of protection correlated with augmented virus-specific CD4<sup>+ </sup>T cell responses and enhanced antibody titers. Similar results were obtained when mice were vaccinated against HIV with adenoviral vectors encoding HIV Env and Gag-Pol in combination with various type I IFN encoding vectors. Here mainly CD4<sup>+ </sup>T cell responses were enhanced by IFNα subtypes.</p> <p>Conclusions</p> <p>Our results indicate that certain IFNα subtypes have the potential to improve the protective effect of adenovirus-based vaccines against retroviruses. This correlated with augmented virus-specific CD4<sup>+ </sup>T cell and antibody responses. Thus, co-expression of select type I IFNs may be a valuable tool for the development of anti-retroviral vaccines.</p>http://www.retrovirology.com/content/8/1/75Friend virusinterferon alpha subtypeshuman adenovirus vectorshuman immunodeficiency virusvaccine |
| spellingShingle | Groitl Peter Schimmer Simone Nabi Ghulam Tenbusch Matthias Johrden Lena Ontikatze Teona Lietz Ruth Bayer Wibke Wolf Hans Berry Cassandra M Überla Klaus Dittmer Ulf Wildner Oliver Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes Retrovirology Friend virus interferon alpha subtypes human adenovirus vectors human immunodeficiency virus vaccine |
| title | Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes |
| title_full | Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes |
| title_fullStr | Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes |
| title_full_unstemmed | Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes |
| title_short | Improved vaccine protection against retrovirus infection after co-administration of adenoviral vectors encoding viral antigens and type I interferon subtypes |
| title_sort | improved vaccine protection against retrovirus infection after co administration of adenoviral vectors encoding viral antigens and type i interferon subtypes |
| topic | Friend virus interferon alpha subtypes human adenovirus vectors human immunodeficiency virus vaccine |
| url | http://www.retrovirology.com/content/8/1/75 |
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