Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia
Abstract Background Listeria monocytogenes (LM) has come to be a major public health issue of at-risk groups, causing high morbidity and mortality. Despite this data, studies are very limited in developing countries like Ethiopia. Thus, we aimed to isolate and characterize LM in terms of antibiogram...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-021-06254-w |
| _version_ | 1818665547302174720 |
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| author | Lencho Girma Alene Geteneh Demisew Amenu Tesfaye Kassa |
| author_facet | Lencho Girma Alene Geteneh Demisew Amenu Tesfaye Kassa |
| author_sort | Lencho Girma |
| collection | DOAJ |
| description | Abstract Background Listeria monocytogenes (LM) has come to be a major public health issue of at-risk groups, causing high morbidity and mortality. Despite this data, studies are very limited in developing countries like Ethiopia. Thus, we aimed to isolate and characterize LM in terms of antibiogram and biofilm formation among pregnant women with fever, women with a history of spontaneous abortion, women with a history of fetal loss, and women with preterm delivery at Jimma University Medical Center (JUMC), southwest Ethiopia. Methods A cross-sectional study was done among 144 women from June to August 2019. Isolates were tested for antibiotic susceptibility and biofilm formation using disc diffusion and microtiter plate method, respectively. Data were collected using a structured questionnaire, entered into Epidata 3.1 and logistic regression was done by SPSS v25.0. Results LM was isolated in 8 (5.56%) of 144 screened women. The isolation rate of LM was relatively higher among women with a history of fetal loss (9.7%), followed by women with preterm delivery (6.25%). One of the six cord blood was positive for LM, indicating that the transplacental transmission rate at JUMC was 16.7%. More than 2% of women with an ongoing pregnancy were found to have LM septicemia, which could hurt their fetus. All of the isolates tested were susceptible to Ampicillin. However, all of the isolates were resistant to Penicillin and Meropenem and were biofilm producers. Conclusions The high magnitude of pregnancy-related listeriosis in the current study setting appears that implementation of educational programs targeting risk reduction and more studies to identify sources of LM are warranted. The choice of antibiotics should be after susceptibility testing. |
| first_indexed | 2024-12-17T05:50:22Z |
| format | Article |
| id | doaj.art-2600d2eb0290455481984f80f1b4807e |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-17T05:50:22Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-2600d2eb0290455481984f80f1b4807e2022-12-21T22:01:10ZengBMCBMC Infectious Diseases1471-23342021-06-012111610.1186/s12879-021-06254-wIsolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest EthiopiaLencho Girma0Alene Geteneh1Demisew Amenu2Tesfaye Kassa3Department of Medical Laboratory Science, Mizan Aman College of Health SciencesDepartment of Medical Laboratory Science, College of Health Sciences, Woldia UniversityDepartment of Obstetrics and Gynecology, School of Medicine, Jimma UniversitySchool of Medical Laboratory Sciences, Jimma UniversityAbstract Background Listeria monocytogenes (LM) has come to be a major public health issue of at-risk groups, causing high morbidity and mortality. Despite this data, studies are very limited in developing countries like Ethiopia. Thus, we aimed to isolate and characterize LM in terms of antibiogram and biofilm formation among pregnant women with fever, women with a history of spontaneous abortion, women with a history of fetal loss, and women with preterm delivery at Jimma University Medical Center (JUMC), southwest Ethiopia. Methods A cross-sectional study was done among 144 women from June to August 2019. Isolates were tested for antibiotic susceptibility and biofilm formation using disc diffusion and microtiter plate method, respectively. Data were collected using a structured questionnaire, entered into Epidata 3.1 and logistic regression was done by SPSS v25.0. Results LM was isolated in 8 (5.56%) of 144 screened women. The isolation rate of LM was relatively higher among women with a history of fetal loss (9.7%), followed by women with preterm delivery (6.25%). One of the six cord blood was positive for LM, indicating that the transplacental transmission rate at JUMC was 16.7%. More than 2% of women with an ongoing pregnancy were found to have LM septicemia, which could hurt their fetus. All of the isolates tested were susceptible to Ampicillin. However, all of the isolates were resistant to Penicillin and Meropenem and were biofilm producers. Conclusions The high magnitude of pregnancy-related listeriosis in the current study setting appears that implementation of educational programs targeting risk reduction and more studies to identify sources of LM are warranted. The choice of antibiotics should be after susceptibility testing.https://doi.org/10.1186/s12879-021-06254-wWomenListeria monocytogenesAntimicrobial susceptibilityBiofilmEthiopia |
| spellingShingle | Lencho Girma Alene Geteneh Demisew Amenu Tesfaye Kassa Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia BMC Infectious Diseases Women Listeria monocytogenes Antimicrobial susceptibility Biofilm Ethiopia |
| title | Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia |
| title_full | Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia |
| title_fullStr | Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia |
| title_full_unstemmed | Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia |
| title_short | Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia |
| title_sort | isolation and characterization of listeria monocytogenes among women attending jimma university medical center southwest ethiopia |
| topic | Women Listeria monocytogenes Antimicrobial susceptibility Biofilm Ethiopia |
| url | https://doi.org/10.1186/s12879-021-06254-w |
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