A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization
Antimicrobial peptides (AMPs) are excellent candidates to fight multi-resistant pathogens worldwide and are considered promising bio-preservatives to control microbial spoilage through food processing. To date, designing de novo AMPs with high therapeutic indexes, low-cost synthesis, high resistance...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/18/6952 |
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| author | Bruna Agrillo Yolande T. R. Proroga Marta Gogliettino Marco Balestrieri Rosarita Tatè Luigi Nicolais Gianna Palmieri |
| author_facet | Bruna Agrillo Yolande T. R. Proroga Marta Gogliettino Marco Balestrieri Rosarita Tatè Luigi Nicolais Gianna Palmieri |
| author_sort | Bruna Agrillo |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) are excellent candidates to fight multi-resistant pathogens worldwide and are considered promising bio-preservatives to control microbial spoilage through food processing. To date, designing de novo AMPs with high therapeutic indexes, low-cost synthesis, high resistance, and bioavailability, remains a challenge. In this study, a novel decapeptide, named RiLK1, was rationally designed starting from the sequence of the previously characterized AMP 1018-K6, with the aim of developing short peptides, and promoting higher selectivity over mammalian cells, antibacterial activity, and structural resistance under different salt, pH, and temperature conditions. Interestingly, RiLK1 displayed a broad-spectrum of bactericidal activity against Gram-positive and Gram-negative bacteria, including multidrug resistant clinical isolates of <i>Salmonella</i> species, with Minimal Bactericidal Concentration (MBC) values in low micromolar range, and it was effective even against two fungal pathogens with no evidence of cytotoxicity on human keratinocytes and fibroblasts. Moreover, RiLK1-activated polypropylene films were revealed to efficiently prevent the growth of microbial spoilage, possibly improving the shelf life of fresh food products. These results suggested that de novo designed peptide RiLK1 could be the first candidate for the development of a promising class of decameric and multitask antimicrobial agents to overcome drug-resistance phenomena. |
| first_indexed | 2024-03-10T16:09:25Z |
| format | Article |
| id | doaj.art-26012ba1fc464308bc6b179e74bcf912 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:09:25Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-26012ba1fc464308bc6b179e74bcf9122023-11-20T14:36:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012118695210.3390/ijms21186952A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional CharacterizationBruna Agrillo0Yolande T. R. Proroga1Marta Gogliettino2Marco Balestrieri3Rosarita Tatè4Luigi Nicolais5Gianna Palmieri6Materias Srl, 80146 Naples, ItalyDepartment of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici, ItalyInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), 80131 Napoli, ItalyInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), 80131 Napoli, ItalyInstitute of Genetics and Biophysics, National Research Council (IGB-CNR), 80131 Naples, ItalyMaterias Srl, 80146 Naples, ItalyInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), 80131 Napoli, ItalyAntimicrobial peptides (AMPs) are excellent candidates to fight multi-resistant pathogens worldwide and are considered promising bio-preservatives to control microbial spoilage through food processing. To date, designing de novo AMPs with high therapeutic indexes, low-cost synthesis, high resistance, and bioavailability, remains a challenge. In this study, a novel decapeptide, named RiLK1, was rationally designed starting from the sequence of the previously characterized AMP 1018-K6, with the aim of developing short peptides, and promoting higher selectivity over mammalian cells, antibacterial activity, and structural resistance under different salt, pH, and temperature conditions. Interestingly, RiLK1 displayed a broad-spectrum of bactericidal activity against Gram-positive and Gram-negative bacteria, including multidrug resistant clinical isolates of <i>Salmonella</i> species, with Minimal Bactericidal Concentration (MBC) values in low micromolar range, and it was effective even against two fungal pathogens with no evidence of cytotoxicity on human keratinocytes and fibroblasts. Moreover, RiLK1-activated polypropylene films were revealed to efficiently prevent the growth of microbial spoilage, possibly improving the shelf life of fresh food products. These results suggested that de novo designed peptide RiLK1 could be the first candidate for the development of a promising class of decameric and multitask antimicrobial agents to overcome drug-resistance phenomena.https://www.mdpi.com/1422-0067/21/18/6952antimicrobial peptidefood pathogensfood packagingantifungal activityMDR <i>Salmonella</i> spp. |
| spellingShingle | Bruna Agrillo Yolande T. R. Proroga Marta Gogliettino Marco Balestrieri Rosarita Tatè Luigi Nicolais Gianna Palmieri A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization International Journal of Molecular Sciences antimicrobial peptide food pathogens food packaging antifungal activity MDR <i>Salmonella</i> spp. |
| title | A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization |
| title_full | A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization |
| title_fullStr | A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization |
| title_full_unstemmed | A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization |
| title_short | A Safe and Multitasking Antimicrobial Decapeptide: The Road from De Novo Design to Structural and Functional Characterization |
| title_sort | safe and multitasking antimicrobial decapeptide the road from de novo design to structural and functional characterization |
| topic | antimicrobial peptide food pathogens food packaging antifungal activity MDR <i>Salmonella</i> spp. |
| url | https://www.mdpi.com/1422-0067/21/18/6952 |
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