iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings
Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-b...
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PeerJ Inc.
2021-06-01
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| Online Access: | https://peerj.com/articles/11509.pdf |
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| author | Jing Wang Chengliang Liang Sha Yang Jingshuang Song Xuefeng Li Xiongze Dai Fei Wang Niran Juntawong Fangjun Tan Xilu Zhang Chunhai Jiao Xuexiao Zou Wenchao Chen |
| author_facet | Jing Wang Chengliang Liang Sha Yang Jingshuang Song Xuefeng Li Xiongze Dai Fei Wang Niran Juntawong Fangjun Tan Xilu Zhang Chunhai Jiao Xuexiao Zou Wenchao Chen |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. |
| first_indexed | 2024-03-09T07:08:04Z |
| format | Article |
| id | doaj.art-2601b28f2ce74eaf8377176cf9dc9856 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:08:04Z |
| publishDate | 2021-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-2601b28f2ce74eaf8377176cf9dc98562023-12-03T09:19:03ZengPeerJ Inc.PeerJ2167-83592021-06-019e1150910.7717/peerj.11509iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlingsJing Wang0Chengliang Liang1Sha Yang2Jingshuang Song3Xuefeng Li4Xiongze Dai5Fei Wang6Niran Juntawong7Fangjun Tan8Xilu Zhang9Chunhai Jiao10Xuexiao Zou11Wenchao Chen12Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCollege of Horticulture and Landscape, Hunan Agricultural University, Changsha, ChinaHubei Academy of Agricultural Sciences, Wuhan, ChinaFaculty of Science, Department of Botany, Kasetsart University, Bangkok, ThailandVegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaHubei Academy of Agricultural Sciences, Wuhan, ChinaCollege of Horticulture and Landscape, Hunan Agricultural University, Changsha, ChinaVegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaBackground As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.https://peerj.com/articles/11509.pdf Pepper Heat stress Physiological Proteomics |
| spellingShingle | Jing Wang Chengliang Liang Sha Yang Jingshuang Song Xuefeng Li Xiongze Dai Fei Wang Niran Juntawong Fangjun Tan Xilu Zhang Chunhai Jiao Xuexiao Zou Wenchao Chen iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings PeerJ Pepper Heat stress Physiological Proteomics |
| title | iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings |
| title_full | iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings |
| title_fullStr | iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings |
| title_full_unstemmed | iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings |
| title_short | iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings |
| title_sort | itraq based quantitative proteomic analysis of heat stress induced mechanisms in pepper seedlings |
| topic | Pepper Heat stress Physiological Proteomics |
| url | https://peerj.com/articles/11509.pdf |
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