Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer
The proteins of extracellular vesicles (EVs) provide proteomic signatures that reflect molecular features of EV-producing cells, including cancer cells. Detection of cancer cell EV proteins is of great interest due to the development of novel predictive diagnostic approaches. Using targeted mass spe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/10/4227 |
| _version_ | 1827740450034810880 |
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| author | Natalia Soloveva Svetlana Novikova Tatiana Farafonova Olga Tikhonova Victor Zgoda |
| author_facet | Natalia Soloveva Svetlana Novikova Tatiana Farafonova Olga Tikhonova Victor Zgoda |
| author_sort | Natalia Soloveva |
| collection | DOAJ |
| description | The proteins of extracellular vesicles (EVs) provide proteomic signatures that reflect molecular features of EV-producing cells, including cancer cells. Detection of cancer cell EV proteins is of great interest due to the development of novel predictive diagnostic approaches. Using targeted mass spectrometry with stable-isotope-labeled peptide standards (SIS), we measured in this study the levels of 34 EV-associated proteins in vesicles and whole lysate derived from the colorectal cancer (CRC) cell lines Caco-2, HT29 and HCT116. We also evaluated the abundance of 13 EV-associated proteins (FN1, TLN1, ITGB3, HSPA8, TUBA4A, CD9, CD63, HSPG2, ITGB1, GNAI2, TSG101, PACSIN2, and CDC42) in EVs isolated from blood plasma samples from 11 CRC patients and 20 healthy volunteers. Downregulation of TLN1, ITGB3, and TUBA4A with simultaneous upregulation of HSPG2 protein were observed in cancer samples compared to healthy controls. The proteomic cargo of the EVs associated with CRC represents a promising source of potential prognostic markers. |
| first_indexed | 2024-03-11T03:26:10Z |
| format | Article |
| id | doaj.art-26077f94298b4e1192d6d17bdb8c2267 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T03:26:10Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-26077f94298b4e1192d6d17bdb8c22672023-11-18T02:41:12ZengMDPI AGMolecules1420-30492023-05-012810422710.3390/molecules28104227Proteomic Signature of Extracellular Vesicles Associated with Colorectal CancerNatalia Soloveva0Svetlana Novikova1Tatiana Farafonova2Olga Tikhonova3Victor Zgoda4Orekhovich Institute of Biomedical Chemistry, Pogodinskaya 10, 119121 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry, Pogodinskaya 10, 119121 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry, Pogodinskaya 10, 119121 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry, Pogodinskaya 10, 119121 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry, Pogodinskaya 10, 119121 Moscow, RussiaThe proteins of extracellular vesicles (EVs) provide proteomic signatures that reflect molecular features of EV-producing cells, including cancer cells. Detection of cancer cell EV proteins is of great interest due to the development of novel predictive diagnostic approaches. Using targeted mass spectrometry with stable-isotope-labeled peptide standards (SIS), we measured in this study the levels of 34 EV-associated proteins in vesicles and whole lysate derived from the colorectal cancer (CRC) cell lines Caco-2, HT29 and HCT116. We also evaluated the abundance of 13 EV-associated proteins (FN1, TLN1, ITGB3, HSPA8, TUBA4A, CD9, CD63, HSPG2, ITGB1, GNAI2, TSG101, PACSIN2, and CDC42) in EVs isolated from blood plasma samples from 11 CRC patients and 20 healthy volunteers. Downregulation of TLN1, ITGB3, and TUBA4A with simultaneous upregulation of HSPG2 protein were observed in cancer samples compared to healthy controls. The proteomic cargo of the EVs associated with CRC represents a promising source of potential prognostic markers.https://www.mdpi.com/1420-3049/28/10/4227extracellular vesiclesproteomic signaturemass spectrometrycolorectal cancerSRMstable-isotope-labeled peptide standards |
| spellingShingle | Natalia Soloveva Svetlana Novikova Tatiana Farafonova Olga Tikhonova Victor Zgoda Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer Molecules extracellular vesicles proteomic signature mass spectrometry colorectal cancer SRM stable-isotope-labeled peptide standards |
| title | Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer |
| title_full | Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer |
| title_fullStr | Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer |
| title_full_unstemmed | Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer |
| title_short | Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer |
| title_sort | proteomic signature of extracellular vesicles associated with colorectal cancer |
| topic | extracellular vesicles proteomic signature mass spectrometry colorectal cancer SRM stable-isotope-labeled peptide standards |
| url | https://www.mdpi.com/1420-3049/28/10/4227 |
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