Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applications
The accumulation of Pb deposits in soil is a growing global concern. Soil remediation options include phytoextraction that involves the use of plants and associated soil microorganism. Switchgrass (Panicum virgatum L.), a second-generation bioenergy crop was used in this study due to its ability to...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Energy Research |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenrg.2022.1032404/full |
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| author | Genna Hart Austin Gilly Marina Koether Thomas McElroy Sigurdur Greipsson |
| author_facet | Genna Hart Austin Gilly Marina Koether Thomas McElroy Sigurdur Greipsson |
| author_sort | Genna Hart |
| collection | DOAJ |
| description | The accumulation of Pb deposits in soil is a growing global concern. Soil remediation options include phytoextraction that involves the use of plants and associated soil microorganism. Switchgrass (Panicum virgatum L.), a second-generation bioenergy crop was used in this study due to its ability to produce high biomass and grow in metal polluted soils. Plants were grown in Pb-contaminated soil (5,802.5 mg kg−1) in an environmentally controlled greenhouse. Plants were treated with exogenous application of the plant growth regulator (PGR) benzylaminopurine (BAP) or complete foliar nutrient solution (Triple-12®) twice a week until harvested. Plants also received the soil fungicide propiconazole (Infuse™) that was followed by the soil chelate nitrilotriacetic acid (NTA). Two concentrations of NTA were compared (5 mM and 10 mM) and combined application of NTA (10 mM) + APG (alkyl polyglucoside). Soil fungicide (propiconazole) was used to arrest arbuscular mycorrhizal fungi (AMF) activities in the roots of switchgrass in order to enhance Pb-phytoextraction. Lead (Pb) was measured in dry plant materials using an ICP-OES. Phytoextraction by switchgrass was significantly improved by dual soil applications of 10 mM NTA, APG and foliar applications of BAP which resulted in the greatestaverage Pb concentration of 5,942 mg kg−1. The average dry mass of plants and the average value for total phytoextracted Pb (mg) per pot were significantly greatestfor plants treated with 10 mM NTA, APG and BAP. Also, plants treated with NTA and BAP showed average bioconcentration factor of 1.02. The results suggested that chemically enhanced phytoextraction significantly improved biomass production of switchgrass and at the same time increased phytoextracted Pb which is important for phytoremediation and bioenergy industry. |
| first_indexed | 2024-04-11T22:54:11Z |
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| issn | 2296-598X |
| language | English |
| last_indexed | 2024-04-11T22:54:11Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Energy Research |
| spelling | doaj.art-260b5347eec04e66be9884997c2ac38e2022-12-22T03:58:28ZengFrontiers Media S.A.Frontiers in Energy Research2296-598X2022-11-011010.3389/fenrg.2022.10324041032404Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applicationsGenna Hart0Austin Gilly1Marina Koether2Thomas McElroy3Sigurdur Greipsson4Department of Ecology, Evolution and Organismal Biology, Kennesaw State University, Kennesaw, GA, United StatesDepartment of Ecology, Evolution and Organismal Biology, Kennesaw State University, Kennesaw, GA, United StatesDepartment of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA, United StatesDepartment of Ecology, Evolution and Organismal Biology, Kennesaw State University, Kennesaw, GA, United StatesDepartment of Ecology, Evolution and Organismal Biology, Kennesaw State University, Kennesaw, GA, United StatesThe accumulation of Pb deposits in soil is a growing global concern. Soil remediation options include phytoextraction that involves the use of plants and associated soil microorganism. Switchgrass (Panicum virgatum L.), a second-generation bioenergy crop was used in this study due to its ability to produce high biomass and grow in metal polluted soils. Plants were grown in Pb-contaminated soil (5,802.5 mg kg−1) in an environmentally controlled greenhouse. Plants were treated with exogenous application of the plant growth regulator (PGR) benzylaminopurine (BAP) or complete foliar nutrient solution (Triple-12®) twice a week until harvested. Plants also received the soil fungicide propiconazole (Infuse™) that was followed by the soil chelate nitrilotriacetic acid (NTA). Two concentrations of NTA were compared (5 mM and 10 mM) and combined application of NTA (10 mM) + APG (alkyl polyglucoside). Soil fungicide (propiconazole) was used to arrest arbuscular mycorrhizal fungi (AMF) activities in the roots of switchgrass in order to enhance Pb-phytoextraction. Lead (Pb) was measured in dry plant materials using an ICP-OES. Phytoextraction by switchgrass was significantly improved by dual soil applications of 10 mM NTA, APG and foliar applications of BAP which resulted in the greatestaverage Pb concentration of 5,942 mg kg−1. The average dry mass of plants and the average value for total phytoextracted Pb (mg) per pot were significantly greatestfor plants treated with 10 mM NTA, APG and BAP. Also, plants treated with NTA and BAP showed average bioconcentration factor of 1.02. The results suggested that chemically enhanced phytoextraction significantly improved biomass production of switchgrass and at the same time increased phytoextracted Pb which is important for phytoremediation and bioenergy industry.https://www.frontiersin.org/articles/10.3389/fenrg.2022.1032404/fullAPGphytoextractionsecond-generation bioenergy cropphytoremediationplant growth regulatorsremediation |
| spellingShingle | Genna Hart Austin Gilly Marina Koether Thomas McElroy Sigurdur Greipsson Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applications Frontiers in Energy Research APG phytoextraction second-generation bioenergy crop phytoremediation plant growth regulators remediation |
| title | Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applications |
| title_full | Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applications |
| title_fullStr | Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applications |
| title_full_unstemmed | Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applications |
| title_short | Phytoextraction of lead (Pb) contaminated soil by switchgrass (Panicum virgatum L): Impact of BAP and NTA applications |
| title_sort | phytoextraction of lead pb contaminated soil by switchgrass panicum virgatum l impact of bap and nta applications |
| topic | APG phytoextraction second-generation bioenergy crop phytoremediation plant growth regulators remediation |
| url | https://www.frontiersin.org/articles/10.3389/fenrg.2022.1032404/full |
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