Stimulation of Human Innate Immune Defenses by Bacteria in vitro
The microflora is essential for immune education and amplification of lymphoid effector cells, mainly at the mucosal level. It is well documented that the efficacy of the mucosal immune system can be significantly impaired in germ-free animals. In this study the bacteria used as antigens (mitogen) t...
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| Format: | Article |
| Language: | English |
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University of Thi-Qar
2019-06-01
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| Series: | مجلة علوم ذي قار |
| Online Access: | http://jsci.utq.edu.iq/index.php/main/article/view/486 |
| _version_ | 1797404230846251008 |
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| author | Manal Badi Salih AL-tmemi |
| author_facet | Manal Badi Salih AL-tmemi |
| author_sort | Manal Badi Salih AL-tmemi |
| collection | DOAJ |
| description | The microflora is essential for immune education and amplification of lymphoid effector cells, mainly at the mucosal level. It is well documented that the efficacy of the mucosal immune system can be significantly impaired in germ-free animals. In this study the bacteria used as antigens (mitogen) to the stimulation of human immune cells in vitro. Gram-positive Streptococci spp and gram-negative Escherichia coli was investigated. The stimulation of human peripheral blood mononuclear cells by the various bacterial strains induced a differential cytokine pattern. Streptococci and E. coli significantly (P ≤ 0.001) induced gamma interferon (IFN-
) and interleukin-12 (IL-12). All bacteria mediated the proliferation of human peripheral blood mononuclear cells. Proliferate activity of lymphocytes was observed when mitogens were added. The MTT is employed to assess the response of lymphocytes to a mitogen. Bacteria and PHA was effective in inducing the MTT index of lymphocytes. Therefore, PHA, gram-positive bacteria, and gram-negative bacteria showed a high significant (P ≤ 0.001) increased percentage of MTT index (96.4, 88.2 and 74.7 %, respectively) as compared to control subjects (42.5%) with no treatment. |
| first_indexed | 2024-03-09T02:51:56Z |
| format | Article |
| id | doaj.art-260f5d2d0ed84b1cb2542fd72bdb2f64 |
| institution | Directory Open Access Journal |
| issn | 1991-8690 2709-0256 |
| language | English |
| last_indexed | 2024-03-09T02:51:56Z |
| publishDate | 2019-06-01 |
| publisher | University of Thi-Qar |
| record_format | Article |
| series | مجلة علوم ذي قار |
| spelling | doaj.art-260f5d2d0ed84b1cb2542fd72bdb2f642023-12-05T09:56:59ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-06-0123Stimulation of Human Innate Immune Defenses by Bacteria in vitroManal Badi Salih AL-tmemi0Medical microbiology / Medicine College / Thi-qar UniversityThe microflora is essential for immune education and amplification of lymphoid effector cells, mainly at the mucosal level. It is well documented that the efficacy of the mucosal immune system can be significantly impaired in germ-free animals. In this study the bacteria used as antigens (mitogen) to the stimulation of human immune cells in vitro. Gram-positive Streptococci spp and gram-negative Escherichia coli was investigated. The stimulation of human peripheral blood mononuclear cells by the various bacterial strains induced a differential cytokine pattern. Streptococci and E. coli significantly (P ≤ 0.001) induced gamma interferon (IFN- ) and interleukin-12 (IL-12). All bacteria mediated the proliferation of human peripheral blood mononuclear cells. Proliferate activity of lymphocytes was observed when mitogens were added. The MTT is employed to assess the response of lymphocytes to a mitogen. Bacteria and PHA was effective in inducing the MTT index of lymphocytes. Therefore, PHA, gram-positive bacteria, and gram-negative bacteria showed a high significant (P ≤ 0.001) increased percentage of MTT index (96.4, 88.2 and 74.7 %, respectively) as compared to control subjects (42.5%) with no treatment.http://jsci.utq.edu.iq/index.php/main/article/view/486 |
| spellingShingle | Manal Badi Salih AL-tmemi Stimulation of Human Innate Immune Defenses by Bacteria in vitro مجلة علوم ذي قار |
| title | Stimulation of Human Innate Immune Defenses by Bacteria in vitro |
| title_full | Stimulation of Human Innate Immune Defenses by Bacteria in vitro |
| title_fullStr | Stimulation of Human Innate Immune Defenses by Bacteria in vitro |
| title_full_unstemmed | Stimulation of Human Innate Immune Defenses by Bacteria in vitro |
| title_short | Stimulation of Human Innate Immune Defenses by Bacteria in vitro |
| title_sort | stimulation of human innate immune defenses by bacteria in vitro |
| url | http://jsci.utq.edu.iq/index.php/main/article/view/486 |
| work_keys_str_mv | AT manalbadisalihaltmemi stimulationofhumaninnateimmunedefensesbybacteriainvitro |