CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINES
This study was aimed to examine the anticancer activity of fabricated ZnONPs- L-ASNase conjugate against cancer and normal cell lines. Total of 127 bacterial isolates were taken from clinicalandenvironmentalsamples then identified as E.coli.In this work, 88 isolates were identified as E.coli.L-aspa...
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| Format: | Article |
| Language: | English |
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Baghdad University
2023-12-01
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| Series: | The Iraqi Journal of Agricultural science |
| Subjects: | |
| Online Access: | https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1866 |
| _version_ | 1827388920470437888 |
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| author | Zahraa H. H. H. A. Rasheed |
| author_facet | Zahraa H. H. H. A. Rasheed |
| author_sort | Zahraa H. H. |
| collection | DOAJ |
| description |
This study was aimed to examine the anticancer activity of fabricated ZnONPs- L-ASNase conjugate against cancer and normal cell lines. Total of 127 bacterial isolates were taken from clinicalandenvironmentalsamples then identified as E.coli.In this work, 88 isolates were identified as E.coli.L-asparaginase produced from E.coli were screened¸clinical Isolates of E.coli from urine sample have the potential to produce asparaginase that is responsible for cytotoxicity¸the optimal culture conditions for the development of the enzyme L-asparaginase were determined to be 37ºC and pH8. Nitrogen sourceconcentrations at 0.9gm/ml were discovered to maximize enzyme production. L-asparaginase then purified by two steps that includeion exchange column and gel filtration. Purification and recovery percentage for L-ASNase was 6.21%, 10.34% respectively. Effective conjugation was approved by UV-visible spectroscopy. ZnO nanoparticles exhibit absorbance peaks approximately 200-300 nm. XRD analysis confirmed the crystallite size of the ZnO nanoparticles was observed to be 18.4 nm. The activity of L-ASNase in the ZnONPs-L-ASNasewas detected by a direct nesslerization method and the findings showed a substantial increase L-ASNase specific activity within the conjugate of ZnONPs-L-ASNase in comparison to its free state. The ZnONPs-L-ASNase conjugate showed the greatest and most substantial cytotoxic action against A375 with survival rate at 400 µg /mL about 45.8± 2.3. The IC50 value of 44.69 µg/ mL of ZnONPs- L-ASNase was calculated for A375 cells. In comparison, all substances exhibited weak to moderate cytotoxicity when tested against the normal cell line WRL-68. The ZnONPs-L-ASNase combination therapy resulted in a higher cytotoxic effect. This action could be attributed to the synergetic effects of both L-ASNase and the conjugated molecules ZnONPs.
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| first_indexed | 2024-03-08T16:27:01Z |
| format | Article |
| id | doaj.art-2616e310d1474b25ae78c12f66d2475a |
| institution | Directory Open Access Journal |
| issn | 0075-0530 2410-0862 |
| language | English |
| last_indexed | 2024-03-08T16:27:01Z |
| publishDate | 2023-12-01 |
| publisher | Baghdad University |
| record_format | Article |
| series | The Iraqi Journal of Agricultural science |
| spelling | doaj.art-2616e310d1474b25ae78c12f66d2475a2024-01-06T18:15:03ZengBaghdad UniversityThe Iraqi Journal of Agricultural science0075-05302410-08622023-12-0154610.36103/ijas.v54i6.1866CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINESZahraa H. H.H. A. Rasheed This study was aimed to examine the anticancer activity of fabricated ZnONPs- L-ASNase conjugate against cancer and normal cell lines. Total of 127 bacterial isolates were taken from clinicalandenvironmentalsamples then identified as E.coli.In this work, 88 isolates were identified as E.coli.L-asparaginase produced from E.coli were screened¸clinical Isolates of E.coli from urine sample have the potential to produce asparaginase that is responsible for cytotoxicity¸the optimal culture conditions for the development of the enzyme L-asparaginase were determined to be 37ºC and pH8. Nitrogen sourceconcentrations at 0.9gm/ml were discovered to maximize enzyme production. L-asparaginase then purified by two steps that includeion exchange column and gel filtration. Purification and recovery percentage for L-ASNase was 6.21%, 10.34% respectively. Effective conjugation was approved by UV-visible spectroscopy. ZnO nanoparticles exhibit absorbance peaks approximately 200-300 nm. XRD analysis confirmed the crystallite size of the ZnO nanoparticles was observed to be 18.4 nm. The activity of L-ASNase in the ZnONPs-L-ASNasewas detected by a direct nesslerization method and the findings showed a substantial increase L-ASNase specific activity within the conjugate of ZnONPs-L-ASNase in comparison to its free state. The ZnONPs-L-ASNase conjugate showed the greatest and most substantial cytotoxic action against A375 with survival rate at 400 µg /mL about 45.8± 2.3. The IC50 value of 44.69 µg/ mL of ZnONPs- L-ASNase was calculated for A375 cells. In comparison, all substances exhibited weak to moderate cytotoxicity when tested against the normal cell line WRL-68. The ZnONPs-L-ASNase combination therapy resulted in a higher cytotoxic effect. This action could be attributed to the synergetic effects of both L-ASNase and the conjugated molecules ZnONPs. https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1866purification, the ZnONPs-L-ASNase conjugate, anticancer activity |
| spellingShingle | Zahraa H. H. H. A. Rasheed CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINES The Iraqi Journal of Agricultural science purification, the ZnONPs-L-ASNase conjugate, anticancer activity |
| title | CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINES |
| title_full | CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINES |
| title_fullStr | CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINES |
| title_full_unstemmed | CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINES |
| title_short | CYTOTOXIC EFFECT OF ASPARAGINASE PRODUCE FROM E.COLI AND CONJUGATED WITH ZNO NANOPARTICLES ON CANCER CELL LINES |
| title_sort | cytotoxic effect of asparaginase produce from e coli and conjugated with zno nanoparticles on cancer cell lines |
| topic | purification, the ZnONPs-L-ASNase conjugate, anticancer activity |
| url | https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1866 |
| work_keys_str_mv | AT zahraahh cytotoxiceffectofasparaginaseproducefromecoliandconjugatedwithznonanoparticlesoncancercelllines AT harasheed cytotoxiceffectofasparaginaseproducefromecoliandconjugatedwithznonanoparticlesoncancercelllines |