Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy women
Abstract Autoantibodies are present in healthy individuals and altered in chronic diseases. We used repeated samples collected from participants in the NYU Women’s Health Study to assess autoantibody reproducibility and repertoire stability over a one-year period using the HuProt array. We included...
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| Format: | Article |
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-10174-3 |
| _version_ | 1828378818441641984 |
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| author | T. V. Clendenen S. Hu Y. Afanasyeva M. Askenazi K. L. Koenig T. Hulett M. Liu S. Liu F. Wu A. Zeleniuch-Jacquotte Y. Chen |
| author_facet | T. V. Clendenen S. Hu Y. Afanasyeva M. Askenazi K. L. Koenig T. Hulett M. Liu S. Liu F. Wu A. Zeleniuch-Jacquotte Y. Chen |
| author_sort | T. V. Clendenen |
| collection | DOAJ |
| description | Abstract Autoantibodies are present in healthy individuals and altered in chronic diseases. We used repeated samples collected from participants in the NYU Women’s Health Study to assess autoantibody reproducibility and repertoire stability over a one-year period using the HuProt array. We included two samples collected one year apart from each of 46 healthy women (92 samples). We also included eight blinded replicate samples to assess laboratory reproducibility. A total of 21,211 IgG and IgM autoantibodies were interrogated. Of those, 86% of IgG (n = 18,303) and 34% of IgM (n = 7,242) autoantibodies showed adequate lab reproducibility (coefficient of variation [CV] < 20%). Intraclass correlation coefficients (ICCs) were estimated to assess temporal reproducibility. A high proportion of both IgG and IgM autoantibodies with CV < 20% (76% and 98%, respectively) showed excellent temporal reproducibility (ICC > 0.8). Temporal reproducibility was lower after using quantile normalization suggesting that batch variability was not an important source of error, and that normalization removed some informative biological information. To our knowledge this study is the largest in terms of sample size and autoantibody numbers to assess autoantibody reproducibility in healthy women. The results suggest that for many autoantibodies a single measurement may be used to rank individuals in studies of autoantibodies as etiologic markers of disease. |
| first_indexed | 2024-12-10T03:29:53Z |
| format | Article |
| id | doaj.art-2617930cd76940e6b436b7ead9477399 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T03:29:53Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-2617930cd76940e6b436b7ead94773992022-12-22T02:03:51ZengNature PortfolioScientific Reports2045-23222022-04-011211910.1038/s41598-022-10174-3Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy womenT. V. Clendenen0S. Hu1Y. Afanasyeva2M. Askenazi3K. L. Koenig4T. Hulett5M. Liu6S. Liu7F. Wu8A. Zeleniuch-Jacquotte9Y. Chen10Department of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineCDI LaboratoriesDepartment of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineDepartment of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineDepartment of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineCDI LaboratoriesDepartment of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineCDI LaboratoriesDepartment of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineDepartment of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineDepartment of Population Health, Division of Epidemiology, NYU Langone Health School of MedicineAbstract Autoantibodies are present in healthy individuals and altered in chronic diseases. We used repeated samples collected from participants in the NYU Women’s Health Study to assess autoantibody reproducibility and repertoire stability over a one-year period using the HuProt array. We included two samples collected one year apart from each of 46 healthy women (92 samples). We also included eight blinded replicate samples to assess laboratory reproducibility. A total of 21,211 IgG and IgM autoantibodies were interrogated. Of those, 86% of IgG (n = 18,303) and 34% of IgM (n = 7,242) autoantibodies showed adequate lab reproducibility (coefficient of variation [CV] < 20%). Intraclass correlation coefficients (ICCs) were estimated to assess temporal reproducibility. A high proportion of both IgG and IgM autoantibodies with CV < 20% (76% and 98%, respectively) showed excellent temporal reproducibility (ICC > 0.8). Temporal reproducibility was lower after using quantile normalization suggesting that batch variability was not an important source of error, and that normalization removed some informative biological information. To our knowledge this study is the largest in terms of sample size and autoantibody numbers to assess autoantibody reproducibility in healthy women. The results suggest that for many autoantibodies a single measurement may be used to rank individuals in studies of autoantibodies as etiologic markers of disease.https://doi.org/10.1038/s41598-022-10174-3 |
| spellingShingle | T. V. Clendenen S. Hu Y. Afanasyeva M. Askenazi K. L. Koenig T. Hulett M. Liu S. Liu F. Wu A. Zeleniuch-Jacquotte Y. Chen Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy women Scientific Reports |
| title | Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy women |
| title_full | Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy women |
| title_fullStr | Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy women |
| title_full_unstemmed | Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy women |
| title_short | Temporal reproducibility of IgG and IgM autoantibodies in serum from healthy women |
| title_sort | temporal reproducibility of igg and igm autoantibodies in serum from healthy women |
| url | https://doi.org/10.1038/s41598-022-10174-3 |
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