Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei

Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei

Objective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM⁃T⁃WaaF cloning plasmid. Downloaded five WaaF associated with Guillain⁃Barré syndrome (GBS) and on...

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Bibliographic Details
Main Authors: Cong⁃cong XING, Fei XU, Xin⁃li BAI, Jin⁃sheng FU, Wen⁃qian LI, Xiao⁃wei LU, Hai⁃yang MA, Zhen⁃zhong LI
Format: Article
Language:English
Published: Tianjin Huanhu Hospital 2012-04-01
Series:Chinese Journal of Contemporary Neurology and Neurosurgery
Subjects:
Campylobacter jejuni
Genes
Sequence analysis, DNA
Evolution
Genetics
Online Access:http://www.cjcnn.org/index.php/cjcnn/article/view/142
Description
Summary:Objective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM⁃T⁃WaaF cloning plasmid. Downloaded five WaaF associated with Guillain⁃Barré syndrome (GBS) and one WaaF not associated with GBS, and then constructed phylogenetic tree. Results pGEM ⁃ T ⁃ WaaF cloning plasmid was constructed successfully. WaaF presented cluster phenomenon in Campylobacter jejuni associated with GBS. Conclusion WaaF gene of Campylobacter jejuni Lulei is the fragment of 807 bp, and has the nearest relationship with the genetic evolution of Lichang. DOI：10.3969/j.issn.1672⁃6731.2012.02.013
ISSN:1672-6731

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