Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei
Objective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM⁃T⁃WaaF cloning plasmid. Downloaded five WaaF associated with Guillain⁃Barré syndrome (GBS) and on...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tianjin Huanhu Hospital
2012-04-01
|
| Series: | Chinese Journal of Contemporary Neurology and Neurosurgery |
| Subjects: | |
| Online Access: | http://www.cjcnn.org/index.php/cjcnn/article/view/142 |
| _version_ | 1819259903135449088 |
|---|---|
| author | Cong⁃cong XING Fei XU Xin⁃li BAI Jin⁃sheng FU Wen⁃qian LI Xiao⁃wei LU Hai⁃yang MA Zhen⁃zhong LI |
| author_facet | Cong⁃cong XING Fei XU Xin⁃li BAI Jin⁃sheng FU Wen⁃qian LI Xiao⁃wei LU Hai⁃yang MA Zhen⁃zhong LI |
| author_sort | Cong⁃cong XING |
| collection | DOAJ |
| description | Objective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM⁃T⁃WaaF cloning plasmid. Downloaded five WaaF associated with Guillain⁃Barré syndrome (GBS) and one WaaF not associated with GBS, and then constructed phylogenetic tree. Results pGEM ⁃ T ⁃ WaaF cloning plasmid was constructed successfully. WaaF presented cluster phenomenon in Campylobacter jejuni associated with GBS. Conclusion WaaF gene of Campylobacter jejuni Lulei is the fragment of 807 bp, and has the nearest relationship with the genetic evolution of Lichang.
DOI：10.3969/j.issn.1672⁃6731.2012.02.013 |
| first_indexed | 2024-12-23T19:17:24Z |
| format | Article |
| id | doaj.art-261ae9557e5d461183b21e16651493c2 |
| institution | Directory Open Access Journal |
| issn | 1672-6731 |
| language | English |
| last_indexed | 2024-12-23T19:17:24Z |
| publishDate | 2012-04-01 |
| publisher | Tianjin Huanhu Hospital |
| record_format | Article |
| series | Chinese Journal of Contemporary Neurology and Neurosurgery |
| spelling | doaj.art-261ae9557e5d461183b21e16651493c22022-12-21T17:34:17ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312012-04-01122158160141Cloning and alignment of WaaF gene of Campylobacter jejuni LuleiCong⁃cong XINGFei XUXin⁃li BAIJin⁃sheng FUWen⁃qian LIXiao⁃wei LUHai⁃yang MAZhen⁃zhong LIObjective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM⁃T⁃WaaF cloning plasmid. Downloaded five WaaF associated with Guillain⁃Barré syndrome (GBS) and one WaaF not associated with GBS, and then constructed phylogenetic tree. Results pGEM ⁃ T ⁃ WaaF cloning plasmid was constructed successfully. WaaF presented cluster phenomenon in Campylobacter jejuni associated with GBS. Conclusion WaaF gene of Campylobacter jejuni Lulei is the fragment of 807 bp, and has the nearest relationship with the genetic evolution of Lichang. DOI：10.3969/j.issn.1672⁃6731.2012.02.013http://www.cjcnn.org/index.php/cjcnn/article/view/142Campylobacter jejuniGenesSequence analysis, DNAEvolutionGenetics |
| spellingShingle | Cong⁃cong XING Fei XU Xin⁃li BAI Jin⁃sheng FU Wen⁃qian LI Xiao⁃wei LU Hai⁃yang MA Zhen⁃zhong LI Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei Chinese Journal of Contemporary Neurology and Neurosurgery Campylobacter jejuni Genes Sequence analysis, DNA Evolution Genetics |
| title | Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei |
| title_full | Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei |
| title_fullStr | Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei |
| title_full_unstemmed | Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei |
| title_short | Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei |
| title_sort | cloning and alignment of waaf gene of campylobacter jejuni lulei |
| topic | Campylobacter jejuni Genes Sequence analysis, DNA Evolution Genetics |
| url | http://www.cjcnn.org/index.php/cjcnn/article/view/142 |
| work_keys_str_mv | AT congcongxing cloningandalignmentofwaafgeneofcampylobacterjejunilulei AT feixu cloningandalignmentofwaafgeneofcampylobacterjejunilulei AT xinlibai cloningandalignmentofwaafgeneofcampylobacterjejunilulei AT jinshengfu cloningandalignmentofwaafgeneofcampylobacterjejunilulei AT wenqianli cloningandalignmentofwaafgeneofcampylobacterjejunilulei AT xiaoweilu cloningandalignmentofwaafgeneofcampylobacterjejunilulei AT haiyangma cloningandalignmentofwaafgeneofcampylobacterjejunilulei AT zhenzhongli cloningandalignmentofwaafgeneofcampylobacterjejunilulei |