The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, Iran
<em><strong>Objective(s):</strong></em> The present study investigated the prevalence of genes encoding for exotoxins, adhesion and biofilm factors in Staphylococcus aureus isolates obtained from samples in a referral burn hospital in Tehran, Iran.<br /><em><st...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2019-11-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_13931_9b268d06e0f0510e28681b1e9dcffe6c.pdf |
| _version_ | 1828414451565461504 |
|---|---|
| author | Zahra Mir Narges Nodeh Farahani Sara Abbasian Faranak Alinejad Mahboubeh Sattarzadeh Ramin Pouriran Mostafa Dahmardehei Mehdi Mirzaii Seyed Sajjad Khoramrooz Davood Darban-Sarokhalil |
| author_facet | Zahra Mir Narges Nodeh Farahani Sara Abbasian Faranak Alinejad Mahboubeh Sattarzadeh Ramin Pouriran Mostafa Dahmardehei Mehdi Mirzaii Seyed Sajjad Khoramrooz Davood Darban-Sarokhalil |
| author_sort | Zahra Mir |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> The present study investigated the prevalence of genes encoding for exotoxins, adhesion and biofilm factors in Staphylococcus aureus isolates obtained from samples in a referral burn hospital in Tehran, Iran.<br /><em><strong>Materials and Methods:</strong></em> S. aureus isolates obtained from patients, personnel and surfaces in the wards of a burn hospital were identified and confirmed by biochemical and molecular tests, respectively. The susceptibility of isolates was determined using the disk diffusion method. Virulence factors were detected by multiplex PCR.<br /><em><strong>Results:</strong></em> The frequency of hla, hlb, hld, hlg, tst and pvl genes was 92.8%, 34.7%, 89.8%, 11.9%, 10.7%, and 0.5% respectively. The results revealed that the hla gene had the highest frequency among isolates (94.4% for methicillin-resistant S. aureus (MRSA) and 89.8% for methicillin-susceptible S. aureus (MSSA)). The most prevalent adhesion and biofilm-related gene was eno (85.6%). The prevalence of the remaining genes was as follows: fib (71.8%), clfB (70%), cna (59.2 %), fnbB (17.9%), icaA (72.4%), and icaD (85.6%). The incidence of fib, hlb, hlg, and tst genes was significantly higher in MRSA isolates compare to the MSSA isolates. Moreover, the resistance rates for all antibiotics were higher is MRSA isolates except for nitrofurantoin and chloramphenicol antibiotics.<br /><em><strong>Conclusion:</strong></em> Data indicate the high prevalence rates of virulence factors among S. aureus isolates, especially MRSA strains in the burn hospital. This should to be taken into account in the development of an effective infection control policy and continuous monitoring of drug resistance in hospitals. |
| first_indexed | 2024-12-10T13:25:14Z |
| format | Article |
| id | doaj.art-261af7834acd4d658eadab08015e96f9 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-10T13:25:14Z |
| publishDate | 2019-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-261af7834acd4d658eadab08015e96f92022-12-22T01:47:10ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742019-11-0122111267127410.22038/ijbms.2019.34908.829113931The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, IranZahra Mir0Narges Nodeh Farahani1Sara Abbasian2Faranak Alinejad3Mahboubeh Sattarzadeh4Ramin Pouriran5Mostafa Dahmardehei6Mehdi Mirzaii7Seyed Sajjad Khoramrooz8Davood Darban-Sarokhalil9Microbial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IranMicrobial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IranMicrobial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IranBurn Research Center, Shahid Motahari Hospital, Iran University of Medical Sciences, Tehran, IranBurn Research Center, Shahid Motahari Hospital, Iran University of Medical Sciences, Tehran, IranSchool of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranBurn Research Center, Shahid Motahari Hospital, Iran University of Medical Sciences, Tehran, IranSchool of Medicine, Shahroud University of Medical Sciences, Shahroud, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranMicrobial Biotechnology Research Center, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran<em><strong>Objective(s):</strong></em> The present study investigated the prevalence of genes encoding for exotoxins, adhesion and biofilm factors in Staphylococcus aureus isolates obtained from samples in a referral burn hospital in Tehran, Iran.<br /><em><strong>Materials and Methods:</strong></em> S. aureus isolates obtained from patients, personnel and surfaces in the wards of a burn hospital were identified and confirmed by biochemical and molecular tests, respectively. The susceptibility of isolates was determined using the disk diffusion method. Virulence factors were detected by multiplex PCR.<br /><em><strong>Results:</strong></em> The frequency of hla, hlb, hld, hlg, tst and pvl genes was 92.8%, 34.7%, 89.8%, 11.9%, 10.7%, and 0.5% respectively. The results revealed that the hla gene had the highest frequency among isolates (94.4% for methicillin-resistant S. aureus (MRSA) and 89.8% for methicillin-susceptible S. aureus (MSSA)). The most prevalent adhesion and biofilm-related gene was eno (85.6%). The prevalence of the remaining genes was as follows: fib (71.8%), clfB (70%), cna (59.2 %), fnbB (17.9%), icaA (72.4%), and icaD (85.6%). The incidence of fib, hlb, hlg, and tst genes was significantly higher in MRSA isolates compare to the MSSA isolates. Moreover, the resistance rates for all antibiotics were higher is MRSA isolates except for nitrofurantoin and chloramphenicol antibiotics.<br /><em><strong>Conclusion:</strong></em> Data indicate the high prevalence rates of virulence factors among S. aureus isolates, especially MRSA strains in the burn hospital. This should to be taken into account in the development of an effective infection control policy and continuous monitoring of drug resistance in hospitals.http://ijbms.mums.ac.ir/article_13931_9b268d06e0f0510e28681b1e9dcffe6c.pdfmrsavirulence factorsadhesin and biofilm genesburniran |
| spellingShingle | Zahra Mir Narges Nodeh Farahani Sara Abbasian Faranak Alinejad Mahboubeh Sattarzadeh Ramin Pouriran Mostafa Dahmardehei Mehdi Mirzaii Seyed Sajjad Khoramrooz Davood Darban-Sarokhalil The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, Iran Iranian Journal of Basic Medical Sciences mrsa virulence factors adhesin and biofilm genes burn iran |
| title | The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, Iran |
| title_full | The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, Iran |
| title_fullStr | The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, Iran |
| title_full_unstemmed | The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, Iran |
| title_short | The prevalence of exotoxins, adhesion, and biofilm-related genes in Staphylococcus aureus isolates from the main burn center of Tehran, Iran |
| title_sort | prevalence of exotoxins adhesion and biofilm related genes in staphylococcus aureus isolates from the main burn center of tehran iran |
| topic | mrsa virulence factors adhesin and biofilm genes burn iran |
| url | http://ijbms.mums.ac.ir/article_13931_9b268d06e0f0510e28681b1e9dcffe6c.pdf |
| work_keys_str_mv | AT zahramir theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT nargesnodehfarahani theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT saraabbasian theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT faranakalinejad theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT mahboubehsattarzadeh theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT raminpouriran theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT mostafadahmardehei theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT mehdimirzaii theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT seyedsajjadkhoramrooz theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT davooddarbansarokhalil theprevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT zahramir prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT nargesnodehfarahani prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT saraabbasian prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT faranakalinejad prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT mahboubehsattarzadeh prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT raminpouriran prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT mostafadahmardehei prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT mehdimirzaii prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT seyedsajjadkhoramrooz prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran AT davooddarbansarokhalil prevalenceofexotoxinsadhesionandbiofilmrelatedgenesinstaphylococcusaureusisolatesfromthemainburncenteroftehraniran |