Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis Subject
Members of the genus <i>Kingella</i> are mostly commensals of the oral cavity, but some of them are involved in invasive infections, especially in young children. This study provides new knowledge on the diversity of this genus by describing a novel species of <i>Kingella</i>...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/10/2/240 |
| _version_ | 1797395894619865088 |
|---|---|
| author | Angéline Antezack Manon Boxberger Clara Rolland Virginie Monnet-Corti Bernard La Scola |
| author_facet | Angéline Antezack Manon Boxberger Clara Rolland Virginie Monnet-Corti Bernard La Scola |
| author_sort | Angéline Antezack |
| collection | DOAJ |
| description | Members of the genus <i>Kingella</i> are mostly commensals of the oral cavity, but some of them are involved in invasive infections, especially in young children. This study provides new knowledge on the diversity of this genus by describing a novel species of <i>Kingella</i> isolated from a dental plaque sample from a 51-year-old man with a history of periodontitis. Morphological and chemotaxonomic characteristic were investigated using different growth conditions, pH and temperature. Cellular fatty acid methyl ester (FAME) analysis was performed by gas chromatography/mass spectrometry (GC/MS). Phylogenetic analysis based on 16S rRNA, orthologous average nucleotide identity (OrthoANI) and digital DNA–DNA hybridization (dDDH) relatedness were also performed. Strain Marseille-Q4569<sup>T</sup> was found to be a facultative aerobic, nonmotile and non-spore-forming rod-shaped bacterium that grows at 28–41.5 °C (optimum 37 °C), pH 5.5–8.5 (optimum pH 7.5) and 5–15 g/L of NaCl. The major fatty acids were Hexadecanoic acid (32.7%), 11-Octadecenoic acid (26.1 %) and 9-Hexadecenoic acid (21.3 %). Despite high 16S rRNA gene sequence similarity (98.72%) between strain Marseille-Q4569<sup>T</sup> and <i>Kingella oralis</i> strain UB-38<sup>T</sup>, the degree of OrthoANI was at the limit of the cutoff (95.83%), and the degree of dDDH was lower (63.6%) than thresholds used to delineate prokaryotic species. Therefore, it is proposed that strain Marseille-Q4569<sup>T</sup> represents a novel species of the genus <i>Kingella</i>, for which the name <i>Kingella bonacorsii</i> sp. nov. is proposed (=CSUR Q4569). |
| first_indexed | 2024-03-09T00:42:40Z |
| format | Article |
| id | doaj.art-261c3849268e4db5af87fe2b627992b5 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T00:42:40Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-261c3849268e4db5af87fe2b627992b52023-12-11T17:43:47ZengMDPI AGPathogens2076-08172021-02-0110224010.3390/pathogens10020240Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis SubjectAngéline Antezack0Manon Boxberger1Clara Rolland2Virginie Monnet-Corti3Bernard La Scola4Faculté des Sciences Médicales et Paramédicales, Ecole de Médecine Dentaire, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille, FranceInstitut de Recherche pour le Développement (IRD), Aix-Marseille Université, Assistance Publique-Hôpitaux de Marseille (AP-HM), MEPHI, 27 Boulevard Jean Moulin, 13005 Marseille, FranceInstitut de Recherche pour le Développement (IRD), Aix-Marseille Université, Assistance Publique-Hôpitaux de Marseille (AP-HM), MEPHI, 27 Boulevard Jean Moulin, 13005 Marseille, FranceFaculté des Sciences Médicales et Paramédicales, Ecole de Médecine Dentaire, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille, FranceInstitut de Recherche pour le Développement (IRD), Aix-Marseille Université, Assistance Publique-Hôpitaux de Marseille (AP-HM), MEPHI, 27 Boulevard Jean Moulin, 13005 Marseille, FranceMembers of the genus <i>Kingella</i> are mostly commensals of the oral cavity, but some of them are involved in invasive infections, especially in young children. This study provides new knowledge on the diversity of this genus by describing a novel species of <i>Kingella</i> isolated from a dental plaque sample from a 51-year-old man with a history of periodontitis. Morphological and chemotaxonomic characteristic were investigated using different growth conditions, pH and temperature. Cellular fatty acid methyl ester (FAME) analysis was performed by gas chromatography/mass spectrometry (GC/MS). Phylogenetic analysis based on 16S rRNA, orthologous average nucleotide identity (OrthoANI) and digital DNA–DNA hybridization (dDDH) relatedness were also performed. Strain Marseille-Q4569<sup>T</sup> was found to be a facultative aerobic, nonmotile and non-spore-forming rod-shaped bacterium that grows at 28–41.5 °C (optimum 37 °C), pH 5.5–8.5 (optimum pH 7.5) and 5–15 g/L of NaCl. The major fatty acids were Hexadecanoic acid (32.7%), 11-Octadecenoic acid (26.1 %) and 9-Hexadecenoic acid (21.3 %). Despite high 16S rRNA gene sequence similarity (98.72%) between strain Marseille-Q4569<sup>T</sup> and <i>Kingella oralis</i> strain UB-38<sup>T</sup>, the degree of OrthoANI was at the limit of the cutoff (95.83%), and the degree of dDDH was lower (63.6%) than thresholds used to delineate prokaryotic species. Therefore, it is proposed that strain Marseille-Q4569<sup>T</sup> represents a novel species of the genus <i>Kingella</i>, for which the name <i>Kingella bonacorsii</i> sp. nov. is proposed (=CSUR Q4569).https://www.mdpi.com/2076-0817/10/2/240<i>Kingella</i>dental plaqueperiodontitisculturomicssp. nov. |
| spellingShingle | Angéline Antezack Manon Boxberger Clara Rolland Virginie Monnet-Corti Bernard La Scola Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis Subject Pathogens <i>Kingella</i> dental plaque periodontitis culturomics sp. nov. |
| title | Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis Subject |
| title_full | Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis Subject |
| title_fullStr | Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis Subject |
| title_full_unstemmed | Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis Subject |
| title_short | Isolation and Characterization of <i>Kingella bonacorsii</i> sp. nov., A Novel <i>Kingella</i> Species Detected in a Stable Periodontitis Subject |
| title_sort | isolation and characterization of i kingella bonacorsii i sp nov a novel i kingella i species detected in a stable periodontitis subject |
| topic | <i>Kingella</i> dental plaque periodontitis culturomics sp. nov. |
| url | https://www.mdpi.com/2076-0817/10/2/240 |
| work_keys_str_mv | AT angelineantezack isolationandcharacterizationofikingellabonacorsiiispnovanovelikingellaispeciesdetectedinastableperiodontitissubject AT manonboxberger isolationandcharacterizationofikingellabonacorsiiispnovanovelikingellaispeciesdetectedinastableperiodontitissubject AT clararolland isolationandcharacterizationofikingellabonacorsiiispnovanovelikingellaispeciesdetectedinastableperiodontitissubject AT virginiemonnetcorti isolationandcharacterizationofikingellabonacorsiiispnovanovelikingellaispeciesdetectedinastableperiodontitissubject AT bernardlascola isolationandcharacterizationofikingellabonacorsiiispnovanovelikingellaispeciesdetectedinastableperiodontitissubject |