Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i>
Plants face multiple stresses in their natural habitats. <i>WRKY</i> transcription factors (TFs) play an important regulatory role in plant stress signaling, regulating the expression of multiple stress-related genes to improve plant stress resistance. In this study, we analyzed the expr...
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MDPI AG
2024-01-01
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| author | Yaning Bao Yifei Zou Xia An Yiwen Liao Lunjin Dai Lijun Liu Dingxiang Peng Xing Huang Bo Wang |
| author_facet | Yaning Bao Yifei Zou Xia An Yiwen Liao Lunjin Dai Lijun Liu Dingxiang Peng Xing Huang Bo Wang |
| author_sort | Yaning Bao |
| collection | DOAJ |
| description | Plants face multiple stresses in their natural habitats. <i>WRKY</i> transcription factors (TFs) play an important regulatory role in plant stress signaling, regulating the expression of multiple stress-related genes to improve plant stress resistance. In this study, we analyzed the expression profiles of 25 <i>BnWRKY</i> genes in three stages of ramie growth (the seedling stage, the rapid-growth stage, and the fiber maturity stage) and response to abiotic stress through qRT-PCR. The results indicated that 25 <i>BnWRKY</i> genes play a role in different growth stages of ramie and were induced by salt and drought stress in the root and leaf. We selected <i>BnWRKY49</i> as a candidate gene for overexpression in Arabidopsis. BnWRKY49 was localized in the nucleus. Overexpression of <i>BnWRKY49</i> affected root elongation under drought and salt stress at the Arabidopsis seedling stage and exhibited increased tolerance to drought stress. Further research found that <i>BnWRKY49</i>-overexpressing lines showed decreased stomatal size and increased cuticular wax deposition under drought compared with wild type (WT). Antioxidant enzyme activities of SOD, POD, and CAT were higher in the <i>BnWRKY49</i>-overexpressing lines than the WT. These findings suggested that the <i>BnWRKY49</i> gene played an important role in drought stress tolerance in Arabidopsis and laid the foundation for further research on the functional analysis of the <i>BnWRKYs</i> in ramie. |
| first_indexed | 2024-03-08T03:50:29Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T03:50:29Z |
| publishDate | 2024-01-01 |
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| series | Plants |
| spelling | doaj.art-2620352d105046599902f6a00c4c0a332024-02-09T15:20:16ZengMDPI AGPlants2223-77472024-01-0113337910.3390/plants13030379Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i>Yaning Bao0Yifei Zou1Xia An2Yiwen Liao3Lunjin Dai4Lijun Liu5Dingxiang Peng6Xing Huang7Bo Wang8Guizhou Key Laboratory for Tobacco Quality Research, College of Tobacco Science, Guizhou University, Guiyang 550025, ChinaRapeseed Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, ChinaZhejiang Xiaoshan Institute of Cotton & Bast Fiber Crops, Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou 311251, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaPlants face multiple stresses in their natural habitats. <i>WRKY</i> transcription factors (TFs) play an important regulatory role in plant stress signaling, regulating the expression of multiple stress-related genes to improve plant stress resistance. In this study, we analyzed the expression profiles of 25 <i>BnWRKY</i> genes in three stages of ramie growth (the seedling stage, the rapid-growth stage, and the fiber maturity stage) and response to abiotic stress through qRT-PCR. The results indicated that 25 <i>BnWRKY</i> genes play a role in different growth stages of ramie and were induced by salt and drought stress in the root and leaf. We selected <i>BnWRKY49</i> as a candidate gene for overexpression in Arabidopsis. BnWRKY49 was localized in the nucleus. Overexpression of <i>BnWRKY49</i> affected root elongation under drought and salt stress at the Arabidopsis seedling stage and exhibited increased tolerance to drought stress. Further research found that <i>BnWRKY49</i>-overexpressing lines showed decreased stomatal size and increased cuticular wax deposition under drought compared with wild type (WT). Antioxidant enzyme activities of SOD, POD, and CAT were higher in the <i>BnWRKY49</i>-overexpressing lines than the WT. These findings suggested that the <i>BnWRKY49</i> gene played an important role in drought stress tolerance in Arabidopsis and laid the foundation for further research on the functional analysis of the <i>BnWRKYs</i> in ramie.https://www.mdpi.com/2223-7747/13/3/379ramieWRKY transcription factorsdroughtsaltstomatal aperture |
| spellingShingle | Yaning Bao Yifei Zou Xia An Yiwen Liao Lunjin Dai Lijun Liu Dingxiang Peng Xing Huang Bo Wang Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i> Plants ramie WRKY transcription factors drought salt stomatal aperture |
| title | Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i> |
| title_full | Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i> |
| title_fullStr | Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i> |
| title_full_unstemmed | Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i> |
| title_short | Overexpression of a Ramie (<i>Boehmaeria nivea</i> L. Gaud) Group I <i>WRKY</i> Gene, <i>BnWRKY49</i>, Increases Drought Resistance in A<i>rabidopsis thaliana</i> |
| title_sort | overexpression of a ramie i boehmaeria nivea i l gaud group i i wrky i gene i bnwrky49 i increases drought resistance in a i rabidopsis thaliana i |
| topic | ramie WRKY transcription factors drought salt stomatal aperture |
| url | https://www.mdpi.com/2223-7747/13/3/379 |
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