Drosophila PTPMT1 Has a Function in Tracheal Air Filling
Summary: The fly trachea is the equivalent of the mammalian lung and is a useful model for human respiratory diseases. However, little is known about the molecular mechanisms underlying tracheal air filling during larval development. In this study, we discover that PTPMT1 has a function in tracheal...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220304727 |
| _version_ | 1818451501079592960 |
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| author | Amanda M. Papakyrikos Min Joo Kim Xinnan Wang |
| author_facet | Amanda M. Papakyrikos Min Joo Kim Xinnan Wang |
| author_sort | Amanda M. Papakyrikos |
| collection | DOAJ |
| description | Summary: The fly trachea is the equivalent of the mammalian lung and is a useful model for human respiratory diseases. However, little is known about the molecular mechanisms underlying tracheal air filling during larval development. In this study, we discover that PTPMT1 has a function in tracheal air filling. PTPMT1 is a widely conserved, ubiquitously expressed mitochondrial phosphatase. To reveal PTPMT1's functions in genetically tractable invertebrates and whether those functions are tissue specific, we generate a Drosophila model of PTPMT1 depletion. We find that fly PTPMT1 mutants show impairments in tracheal air filling and subsequent activation of innate immune responses. On a cellular level, these defects are preceded by aggregation of mitochondria within the tracheal epithelial cells. Our work demonstrates a cell-type-specific role for PTPMT1 in fly tracheal epithelial cells to support air filling and to prevent immune activation. The establishment of this model will facilitate exploration of PTPMT1's physiological functions in vivo. |
| first_indexed | 2024-12-14T21:08:12Z |
| format | Article |
| id | doaj.art-2620647439d74e7a95a71ed747dda782 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-14T21:08:12Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-2620647439d74e7a95a71ed747dda7822022-12-21T22:47:20ZengElsevieriScience2589-00422020-07-01237101285Drosophila PTPMT1 Has a Function in Tracheal Air FillingAmanda M. Papakyrikos0Min Joo Kim1Xinnan Wang2Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Graduate Program in Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Corresponding authorSummary: The fly trachea is the equivalent of the mammalian lung and is a useful model for human respiratory diseases. However, little is known about the molecular mechanisms underlying tracheal air filling during larval development. In this study, we discover that PTPMT1 has a function in tracheal air filling. PTPMT1 is a widely conserved, ubiquitously expressed mitochondrial phosphatase. To reveal PTPMT1's functions in genetically tractable invertebrates and whether those functions are tissue specific, we generate a Drosophila model of PTPMT1 depletion. We find that fly PTPMT1 mutants show impairments in tracheal air filling and subsequent activation of innate immune responses. On a cellular level, these defects are preceded by aggregation of mitochondria within the tracheal epithelial cells. Our work demonstrates a cell-type-specific role for PTPMT1 in fly tracheal epithelial cells to support air filling and to prevent immune activation. The establishment of this model will facilitate exploration of PTPMT1's physiological functions in vivo.http://www.sciencedirect.com/science/article/pii/S2589004220304727Biological SciencesMolecular BiologyCell Biology |
| spellingShingle | Amanda M. Papakyrikos Min Joo Kim Xinnan Wang Drosophila PTPMT1 Has a Function in Tracheal Air Filling iScience Biological Sciences Molecular Biology Cell Biology |
| title | Drosophila PTPMT1 Has a Function in Tracheal Air Filling |
| title_full | Drosophila PTPMT1 Has a Function in Tracheal Air Filling |
| title_fullStr | Drosophila PTPMT1 Has a Function in Tracheal Air Filling |
| title_full_unstemmed | Drosophila PTPMT1 Has a Function in Tracheal Air Filling |
| title_short | Drosophila PTPMT1 Has a Function in Tracheal Air Filling |
| title_sort | drosophila ptpmt1 has a function in tracheal air filling |
| topic | Biological Sciences Molecular Biology Cell Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004220304727 |
| work_keys_str_mv | AT amandampapakyrikos drosophilaptpmt1hasafunctionintrachealairfilling AT minjookim drosophilaptpmt1hasafunctionintrachealairfilling AT xinnanwang drosophilaptpmt1hasafunctionintrachealairfilling |