Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Nucleus |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19491034.2022.2034286 |
| _version_ | 1818984837067833344 |
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| author | Pallavi Deolal Imlitoshi Jamir Krishnaveni Mishra |
| author_facet | Pallavi Deolal Imlitoshi Jamir Krishnaveni Mishra |
| author_sort | Pallavi Deolal |
| collection | DOAJ |
| description | A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In a quest to identify factors that contribute to regulation of nuclear shape and function in Saccharomyces cerevisiae, we used a fluorescence imaging based approach. Here we report the identification of a novel protein, Uip4p, that is required for regulation of nuclear morphology. Loss of Uip4 compromises NPC function and loss of nuclear envelope (NE) integrity. Our localization studies show that Uip4 localizes to the NE and endoplasmic reticulum (ER) network. Furthermore, we demonstrate that the localization and expression of Uip4 is regulated during growth, which is crucial for NPC distribution. |
| first_indexed | 2024-12-20T18:25:21Z |
| format | Article |
| id | doaj.art-2620f6699dae40d7ad86bc64535b0977 |
| institution | Directory Open Access Journal |
| issn | 1949-1034 1949-1042 |
| language | English |
| last_indexed | 2024-12-20T18:25:21Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Nucleus |
| spelling | doaj.art-2620f6699dae40d7ad86bc64535b09772022-12-21T19:30:11ZengTaylor & Francis GroupNucleus1949-10341949-10422022-12-01131799310.1080/19491034.2022.20342862034286Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiaePallavi Deolal0Imlitoshi Jamir1Krishnaveni Mishra2University of HyderabadNagaland UniversityUniversity of HyderabadA double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In a quest to identify factors that contribute to regulation of nuclear shape and function in Saccharomyces cerevisiae, we used a fluorescence imaging based approach. Here we report the identification of a novel protein, Uip4p, that is required for regulation of nuclear morphology. Loss of Uip4 compromises NPC function and loss of nuclear envelope (NE) integrity. Our localization studies show that Uip4 localizes to the NE and endoplasmic reticulum (ER) network. Furthermore, we demonstrate that the localization and expression of Uip4 is regulated during growth, which is crucial for NPC distribution.http://dx.doi.org/10.1080/19491034.2022.2034286nuclear envelopenuclear pore complexyeastuip4 |
| spellingShingle | Pallavi Deolal Imlitoshi Jamir Krishnaveni Mishra Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae Nucleus nuclear envelope nuclear pore complex yeast uip4 |
| title | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
| title_full | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
| title_fullStr | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
| title_full_unstemmed | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
| title_short | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
| title_sort | uip4p modulates nuclear pore complex function in saccharomyces cerevisiae |
| topic | nuclear envelope nuclear pore complex yeast uip4 |
| url | http://dx.doi.org/10.1080/19491034.2022.2034286 |
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