Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup>
A hybrid noble nanoparticle/DNAzyme electrochemical biosensor is proposed for the detection of Pb<sup>2+</sup>, Cd<sup>2+</sup>, and Cr<sup>3+</sup>. The sensor takes advantage of a well-studied material that is known for its selective interaction with heavy metal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/18/7818 |
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| author | Evangelos Skotadis Evangelos Aslanidis Georgios Tsekenis Chryssi Panagopoulou Annita Rapesi Georgia Tzourmana Stella Kennou Spyridon Ladas Angelos Zeniou Dimitris Tsoukalas |
| author_facet | Evangelos Skotadis Evangelos Aslanidis Georgios Tsekenis Chryssi Panagopoulou Annita Rapesi Georgia Tzourmana Stella Kennou Spyridon Ladas Angelos Zeniou Dimitris Tsoukalas |
| author_sort | Evangelos Skotadis |
| collection | DOAJ |
| description | A hybrid noble nanoparticle/DNAzyme electrochemical biosensor is proposed for the detection of Pb<sup>2+</sup>, Cd<sup>2+</sup>, and Cr<sup>3+</sup>. The sensor takes advantage of a well-studied material that is known for its selective interaction with heavy metal ions (i.e., DNAzymes), which is combined with metallic nanoparticles. The double-helix structure of DNAzymes is known to dissociate into smaller fragments in the presence of specific heavy metal ions; this results in a measurable change in device resistance due to the collapse of conductive inter-nanoparticle DNAzyme bridging. The paper discusses the effect of DNAzyme anchoring groups (i.e., thiol and amino functionalization groups) on device performance and reports on the successful detection of all three target ions in concentrations that are well below their maximum permitted levels in tap water. While the use of DNAzymes for the detection of lead in particular and, to some extent, cadmium has been studied extensively, this is one of the few reports on the successful detection of chromium (III) via a sensor incorporating DNAzymes. The sensor showed great potential for its future integration in autonomous and remote sensing systems due to its low power characteristics, simple and cost-effective fabrication, and easy automation and measurement. |
| first_indexed | 2024-03-10T22:02:04Z |
| format | Article |
| id | doaj.art-2622099027dc4d89972453d981e09a83 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T22:02:04Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-2622099027dc4d89972453d981e09a832023-11-19T12:54:39ZengMDPI AGSensors1424-82202023-09-012318781810.3390/s23187818Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup>Evangelos Skotadis0Evangelos Aslanidis1Georgios Tsekenis2Chryssi Panagopoulou3Annita Rapesi4Georgia Tzourmana5Stella Kennou6Spyridon Ladas7Angelos Zeniou8Dimitris Tsoukalas9Department of Applied Physics, National Technical University of Athens, 15780 Athens, GreeceDepartment of Applied Physics, National Technical University of Athens, 15780 Athens, GreeceBiomedical Research Foundation, Academy of Athens, 4 Soranou Ephessiou Street, 11527 Athens, GreeceDepartment of Applied Physics, National Technical University of Athens, 15780 Athens, GreeceDepartment of Applied Physics, National Technical University of Athens, 15780 Athens, GreeceDepartment of Applied Physics, National Technical University of Athens, 15780 Athens, GreeceDepartment of Chemical Engineering, University of Patras, 26504 Patras, GreeceDepartment of Chemical Engineering, University of Patras, 26504 Patras, GreeceInstitute of Nanoscience and Nanotechnology, NCSR Demokritos, Aghia Paraskevi, 15310 Attiki, GreeceDepartment of Applied Physics, National Technical University of Athens, 15780 Athens, GreeceA hybrid noble nanoparticle/DNAzyme electrochemical biosensor is proposed for the detection of Pb<sup>2+</sup>, Cd<sup>2+</sup>, and Cr<sup>3+</sup>. The sensor takes advantage of a well-studied material that is known for its selective interaction with heavy metal ions (i.e., DNAzymes), which is combined with metallic nanoparticles. The double-helix structure of DNAzymes is known to dissociate into smaller fragments in the presence of specific heavy metal ions; this results in a measurable change in device resistance due to the collapse of conductive inter-nanoparticle DNAzyme bridging. The paper discusses the effect of DNAzyme anchoring groups (i.e., thiol and amino functionalization groups) on device performance and reports on the successful detection of all three target ions in concentrations that are well below their maximum permitted levels in tap water. While the use of DNAzymes for the detection of lead in particular and, to some extent, cadmium has been studied extensively, this is one of the few reports on the successful detection of chromium (III) via a sensor incorporating DNAzymes. The sensor showed great potential for its future integration in autonomous and remote sensing systems due to its low power characteristics, simple and cost-effective fabrication, and easy automation and measurement.https://www.mdpi.com/1424-8220/23/18/7818heavy metalchromiumbiosensorDNAzymenanoparticles |
| spellingShingle | Evangelos Skotadis Evangelos Aslanidis Georgios Tsekenis Chryssi Panagopoulou Annita Rapesi Georgia Tzourmana Stella Kennou Spyridon Ladas Angelos Zeniou Dimitris Tsoukalas Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup> Sensors heavy metal chromium biosensor DNAzyme nanoparticles |
| title | Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup> |
| title_full | Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup> |
| title_fullStr | Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup> |
| title_full_unstemmed | Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup> |
| title_short | Hybrid Nanoparticle/DNAzyme Electrochemical Biosensor for the Detection of Divalent Heavy Metal Ions and Cr<sup>3+</sup> |
| title_sort | hybrid nanoparticle dnazyme electrochemical biosensor for the detection of divalent heavy metal ions and cr sup 3 sup |
| topic | heavy metal chromium biosensor DNAzyme nanoparticles |
| url | https://www.mdpi.com/1424-8220/23/18/7818 |
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