ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY
Aim. To analyze differential expression of metalloproteases genes, involved into the processes of stabilization/destabilization of atherosclerotic plaque, with the method of full genome sequencing of RNA, and to evaluate the level of metalloproteases in homogenates of plaques of various types by imm...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
«FIRMA «SILICEA» LLC
2018-09-01
|
| Series: | Российский кардиологический журнал |
| Subjects: | |
| Online Access: | https://russjcardiol.elpub.ru/jour/article/view/2877 |
| _version_ | 1797856868255662080 |
|---|---|
| author | D. Е. Ivanoschuk Yu. I. Ragino E. V. Shakhtshneider S. V. Mikhailova V. S. Fishman Ya. V. Polonskaya E. V. Kashtanova A. M. Chernyavsky I. S. Murashov М. I. Voevoda |
| author_facet | D. Е. Ivanoschuk Yu. I. Ragino E. V. Shakhtshneider S. V. Mikhailova V. S. Fishman Ya. V. Polonskaya E. V. Kashtanova A. M. Chernyavsky I. S. Murashov М. I. Voevoda |
| author_sort | D. Е. Ivanoschuk |
| collection | DOAJ |
| description | Aim. To analyze differential expression of metalloproteases genes, involved into the processes of stabilization/destabilization of atherosclerotic plaque, with the method of full genome sequencing of RNA, and to evaluate the level of metalloproteases in homogenates of plaques of various types by immune enzyme assay method (IEA).Material and methods. The study has been conducted on the specimens of atherosclerotic plaques of patients aged 45-65 y.o., inhabitants of Western Siberia with angiographically proven coronary atherosclerosis and no acute coronary syndrome, with stable angina II-IV functional class. Specimens collection from the plaques was done during an operation if there were intraoperational indications. Histology performed. In intima/media homogenates by IEA method, with BCM Diagnostics assays the levels of destructive markers were measured: MMP-1, MMP3, MMP-7, MMP-9, TIMP-1 on the Multiscan EX (Thermo Fisher Scientific, USA). Libraries preparation for full genomic sequencing of RNA was done with Illumina’s TruSeq RNA Sample Preparation Kit (Illumina, USA). Expression profile in tissues was done on HiSeq 1500 (Illumina, USA).Results. There are differences in expression of the genes MMP2, MMP7, MMP8, MMP9, MMP12, и MMP14 in different types of plaques. There was 8 times higher significant raise in increase of the expression level of ММР9 (p<0,001) in unstable plaque of dystrophic-necrotic type. Study by IEA of MMP-7 content, which is an activator of pro-MMP-9, as well as the content of MMP-9 itself, showed their increased levels in unstable plaques comparing to fatty streaks (1,5 and 2,4 times) and young stable plaques (1,4 and 2,1 times).Conclusion. For the gene ММР9 there were significant differences obtained, of expression levels in stable atherosclerotic fibrous plaque and unstable plaque of dystrophic-necrotic type. With the IEA it was found that fatty streaks and young stable atheromas of coronary arteries have an increased concentration of MMP-3 and decreased activity of tissue inhibitor of metalloproteases. In unstable plaques with the tendency to rupture/ulceration there are increased levels of MMP-1, MMP-7, MMP-9. |
| first_indexed | 2024-04-09T20:47:30Z |
| format | Article |
| id | doaj.art-26231365a24f4745ba70b31598c2311c |
| institution | Directory Open Access Journal |
| issn | 1560-4071 2618-7620 |
| language | Russian |
| last_indexed | 2024-04-09T20:47:30Z |
| publishDate | 2018-09-01 |
| publisher | «FIRMA «SILICEA» LLC |
| record_format | Article |
| series | Российский кардиологический журнал |
| spelling | doaj.art-26231365a24f4745ba70b31598c2311c2023-03-29T21:23:30Zrus«FIRMA «SILICEA» LLCРоссийский кардиологический журнал1560-40712618-76202018-09-0108525810.15829/1560-4071-2018-8-52-582455ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDYD. Е. Ivanoschuk0Yu. I. Ragino1E. V. Shakhtshneider2S. V. Mikhailova3V. S. Fishman4Ya. V. Polonskaya5E. V. Kashtanova6A. M. Chernyavsky7I. S. Murashov8М. I. Voevoda9Scientific Research Institute of Therapy and Prevention Medicine — branch of FRCCG SD RAS; Federal Research Center for Cytology and Genetics (FRCCG) of SD RAS; Novosibirsk National Research State University,Scientific Research Institute of Therapy and Prevention Medicine — branch of FRCCG SD RAS; Federal Research Center for Cytology and Genetics (FRCCG) of SD RASScientific Research Institute of Therapy and Prevention Medicine — branch of FRCCG SD RAS; Federal Research Center for Cytology and Genetics (FRCCG) of SD RAS; Novosibirsk National Research State UniversityScientific Research Institute of Therapy and Prevention Medicine — branch of FRCCG SD RAS; Federal Research Center for Cytology and Genetics (FRCCG) of SD RASCenter for Cytology and Genetics (FRCCG) of SD RAS; Novosibirsk National Research State UniversityScientific Research Institute of Therapy and Prevention Medicine — branch of FRCCG SD RAS; Federal Research Center for Cytology and Genetics (FRCCG) of SD RASScientific Research Institute of Therapy and Prevention Medicine — branch of FRCCG SD RAS; Federal Research Center for Cytology and Genetics (FRCCG) of SD RASE.N. Meshalkin Novosibirsk National Medical Research InstituteE.N. Meshalkin Novosibirsk National Medical Research InstituteScientific Research Institute of Therapy and Prevention Medicine — branch of FRCCG SD RAS; Federal Research Center for Cytology and Genetics (FRCCG) of SD RAS; Novosibirsk National Research State University,Aim. To analyze differential expression of metalloproteases genes, involved into the processes of stabilization/destabilization of atherosclerotic plaque, with the method of full genome sequencing of RNA, and to evaluate the level of metalloproteases in homogenates of plaques of various types by immune enzyme assay method (IEA).Material and methods. The study has been conducted on the specimens of atherosclerotic plaques of patients aged 45-65 y.o., inhabitants of Western Siberia with angiographically proven coronary atherosclerosis and no acute coronary syndrome, with stable angina II-IV functional class. Specimens collection from the plaques was done during an operation if there were intraoperational indications. Histology performed. In intima/media homogenates by IEA method, with BCM Diagnostics assays the levels of destructive markers were measured: MMP-1, MMP3, MMP-7, MMP-9, TIMP-1 on the Multiscan EX (Thermo Fisher Scientific, USA). Libraries preparation for full genomic sequencing of RNA was done with Illumina’s TruSeq RNA Sample Preparation Kit (Illumina, USA). Expression profile in tissues was done on HiSeq 1500 (Illumina, USA).Results. There are differences in expression of the genes MMP2, MMP7, MMP8, MMP9, MMP12, и MMP14 in different types of plaques. There was 8 times higher significant raise in increase of the expression level of ММР9 (p<0,001) in unstable plaque of dystrophic-necrotic type. Study by IEA of MMP-7 content, which is an activator of pro-MMP-9, as well as the content of MMP-9 itself, showed their increased levels in unstable plaques comparing to fatty streaks (1,5 and 2,4 times) and young stable plaques (1,4 and 2,1 times).Conclusion. For the gene ММР9 there were significant differences obtained, of expression levels in stable atherosclerotic fibrous plaque and unstable plaque of dystrophic-necrotic type. With the IEA it was found that fatty streaks and young stable atheromas of coronary arteries have an increased concentration of MMP-3 and decreased activity of tissue inhibitor of metalloproteases. In unstable plaques with the tendency to rupture/ulceration there are increased levels of MMP-1, MMP-7, MMP-9.https://russjcardiol.elpub.ru/jour/article/view/2877matrix metalloproteasesatherosclerotic plaquesrnafull genomic sequencing of rnatranscriptomeatherosclerosis |
| spellingShingle | D. Е. Ivanoschuk Yu. I. Ragino E. V. Shakhtshneider S. V. Mikhailova V. S. Fishman Ya. V. Polonskaya E. V. Kashtanova A. M. Chernyavsky I. S. Murashov М. I. Voevoda ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY Российский кардиологический журнал matrix metalloproteases atherosclerotic plaques rna full genomic sequencing of rna transcriptome atherosclerosis |
| title | ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY |
| title_full | ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY |
| title_fullStr | ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY |
| title_full_unstemmed | ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY |
| title_short | ANALYSIS OF DIFFERENTIAL ExPRESSION OF MATRIx METALLOPROTEASES IN STABLE AND UNSTABLE ATHEROSCLEROTIC LESIONS BY A METHOD OF FULL GENOME SEQUENCING OF RNA: PILOT STUDY |
| title_sort | analysis of differential expression of matrix metalloproteases in stable and unstable atherosclerotic lesions by a method of full genome sequencing of rna pilot study |
| topic | matrix metalloproteases atherosclerotic plaques rna full genomic sequencing of rna transcriptome atherosclerosis |
| url | https://russjcardiol.elpub.ru/jour/article/view/2877 |
| work_keys_str_mv | AT deivanoschuk analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT yuiragino analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT evshakhtshneider analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT svmikhailova analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT vsfishman analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT yavpolonskaya analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT evkashtanova analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT amchernyavsky analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT ismurashov analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy AT mivoevoda analysisofdifferentialexpressionofmatrixmetalloproteasesinstableandunstableatheroscleroticlesionsbyamethodoffullgenomesequencingofrnapilotstudy |