Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018)
Abstract Background ESKAPEE pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli are multi-drug resistant (MDR) bacteria that present increasing treatment challenges for healthcare instit...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-022-07678-8 |
| _version_ | 1828322452168507392 |
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| author | Sirigade Ruekit Apichai Srijan Oralak Serichantalergs Katie R. Margulieux Patrick Mc Gann Emma G. Mills William C. Stribling Theerasak Pimsawat Rosarin Kormanee Suthisak Nakornchai Chaiwat Sakdinava Prawet Sukhchat Mariusz Wojnarski Samandra T. Demons John M. Crawford Paphavee Lertsethtakarn Brett E. Swierczewski |
| author_facet | Sirigade Ruekit Apichai Srijan Oralak Serichantalergs Katie R. Margulieux Patrick Mc Gann Emma G. Mills William C. Stribling Theerasak Pimsawat Rosarin Kormanee Suthisak Nakornchai Chaiwat Sakdinava Prawet Sukhchat Mariusz Wojnarski Samandra T. Demons John M. Crawford Paphavee Lertsethtakarn Brett E. Swierczewski |
| author_sort | Sirigade Ruekit |
| collection | DOAJ |
| description | Abstract Background ESKAPEE pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli are multi-drug resistant (MDR) bacteria that present increasing treatment challenges for healthcare institutions and public health worldwide. Methods 431 MDR ESKAPEE pathogens were collected from Queen Sirikit Naval Hospital, Chonburi, Thailand between 2017 and 2018. Species identification and antimicrobial resistance (AMR) phenotype were determined following CLSI and EUCAST guidelines on the BD Phoenix System. Molecular identification of antibiotic resistant genes was performed by polymerase chain reaction (PCR), real-time PCR assays, and whole genome sequencing (WGS). Results Of the 431 MDR isolates collected, 1.2% were E. faecium, 5.8% were S. aureus, 23.7% were K. pneumoniae, 22.5% were A. baumannii, 4.6% were P. aeruginosa, 0.9% were Enterobacter spp., and 41.3% were E. coli. Of the 401 Gram-negative MDR isolates, 51% were carbapenem resistant, 45% were ESBL producers only, 2% were colistin resistance and ESBLs producers (2%), and 2% were non-ESBLs producers. The most prevalent carbapenemase genes were bla OXA-23 (23%), which was only identified in A. baumannii, followed by bla NDM (17%), and bla OXA-48-like (13%). Beta-lactamase genes detected included bla TEM, bla SHV, bla OXA, bla CTX-M, bla DHA, bla CMY, bla PER and bla VEB. Seven E. coli and K. pneumoniae isolates showed resistance to colistin and carried mcr-1 or mcr-3, with 2 E. coli strains carrying both genes. Among 30 Gram-positive MDR ESKAPEE, all VRE isolates carried the vanA gene (100%) and 84% S. aureus isolates carried the mecA gene. Conclusions This report highlights the prevalence of AMR among clinical ESKAPEE pathogens in eastern Thailand. E. coli was the most common MDR pathogen collected, followed by K. pneumoniae, and A. baumannii. Carbapenem-resistant Enterobacteriaceae (CRE) and extended spectrum beta-lactamases (ESBLs) producers were the most common resistance profiles. The co-occurrence of mcr-1 and mcr-3 in 2 E. coli strains, which did not affect the level of colistin resistance, is also reported. The participation of global stakeholders and surveillance of MDR remain essential for the control and management of MDR ESKAPEE pathogens. |
| first_indexed | 2024-04-13T18:40:30Z |
| format | Article |
| id | doaj.art-26241a982d6447a39d28a605f9fea4b6 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T18:40:30Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-26241a982d6447a39d28a605f9fea4b62022-12-22T02:34:44ZengBMCBMC Infectious Diseases1471-23342022-08-0122111210.1186/s12879-022-07678-8Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018)Sirigade Ruekit0Apichai Srijan1Oralak Serichantalergs2Katie R. Margulieux3Patrick Mc Gann4Emma G. Mills5William C. Stribling6Theerasak Pimsawat7Rosarin Kormanee8Suthisak Nakornchai9Chaiwat Sakdinava10Prawet Sukhchat11Mariusz Wojnarski12Samandra T. Demons13John M. Crawford14Paphavee Lertsethtakarn15Brett E. Swierczewski16Bacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS)Bacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS)Bacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS)Wound Infections Department, Bacterial Diseases Branch, Walter Reed Army Institute of ResearchMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of ResearchMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of ResearchMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of ResearchQueen Sirikit Naval HospitalQueen Sirikit Naval HospitalQueen Sirikit Naval HospitalQueen Sirikit Naval HospitalQueen Sirikit Naval HospitalBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS)Bacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS)US Army Medical Research Institute of Chemical DefenseBacterial and Parasitic Diseases Department, Armed Forces Research Institute of Medical Sciences (AFRIMS)Bacterial Diseases Branch, Walter Reed Army Institute of ResearchAbstract Background ESKAPEE pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli are multi-drug resistant (MDR) bacteria that present increasing treatment challenges for healthcare institutions and public health worldwide. Methods 431 MDR ESKAPEE pathogens were collected from Queen Sirikit Naval Hospital, Chonburi, Thailand between 2017 and 2018. Species identification and antimicrobial resistance (AMR) phenotype were determined following CLSI and EUCAST guidelines on the BD Phoenix System. Molecular identification of antibiotic resistant genes was performed by polymerase chain reaction (PCR), real-time PCR assays, and whole genome sequencing (WGS). Results Of the 431 MDR isolates collected, 1.2% were E. faecium, 5.8% were S. aureus, 23.7% were K. pneumoniae, 22.5% were A. baumannii, 4.6% were P. aeruginosa, 0.9% were Enterobacter spp., and 41.3% were E. coli. Of the 401 Gram-negative MDR isolates, 51% were carbapenem resistant, 45% were ESBL producers only, 2% were colistin resistance and ESBLs producers (2%), and 2% were non-ESBLs producers. The most prevalent carbapenemase genes were bla OXA-23 (23%), which was only identified in A. baumannii, followed by bla NDM (17%), and bla OXA-48-like (13%). Beta-lactamase genes detected included bla TEM, bla SHV, bla OXA, bla CTX-M, bla DHA, bla CMY, bla PER and bla VEB. Seven E. coli and K. pneumoniae isolates showed resistance to colistin and carried mcr-1 or mcr-3, with 2 E. coli strains carrying both genes. Among 30 Gram-positive MDR ESKAPEE, all VRE isolates carried the vanA gene (100%) and 84% S. aureus isolates carried the mecA gene. Conclusions This report highlights the prevalence of AMR among clinical ESKAPEE pathogens in eastern Thailand. E. coli was the most common MDR pathogen collected, followed by K. pneumoniae, and A. baumannii. Carbapenem-resistant Enterobacteriaceae (CRE) and extended spectrum beta-lactamases (ESBLs) producers were the most common resistance profiles. The co-occurrence of mcr-1 and mcr-3 in 2 E. coli strains, which did not affect the level of colistin resistance, is also reported. The participation of global stakeholders and surveillance of MDR remain essential for the control and management of MDR ESKAPEE pathogens.https://doi.org/10.1186/s12879-022-07678-8MDRESKAPEECREESBLmcrThailand |
| spellingShingle | Sirigade Ruekit Apichai Srijan Oralak Serichantalergs Katie R. Margulieux Patrick Mc Gann Emma G. Mills William C. Stribling Theerasak Pimsawat Rosarin Kormanee Suthisak Nakornchai Chaiwat Sakdinava Prawet Sukhchat Mariusz Wojnarski Samandra T. Demons John M. Crawford Paphavee Lertsethtakarn Brett E. Swierczewski Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018) BMC Infectious Diseases MDR ESKAPEE CRE ESBL mcr Thailand |
| title | Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018) |
| title_full | Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018) |
| title_fullStr | Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018) |
| title_full_unstemmed | Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018) |
| title_short | Molecular characterization of multidrug-resistant ESKAPEE pathogens from clinical samples in Chonburi, Thailand (2017–2018) |
| title_sort | molecular characterization of multidrug resistant eskapee pathogens from clinical samples in chonburi thailand 2017 2018 |
| topic | MDR ESKAPEE CRE ESBL mcr Thailand |
| url | https://doi.org/10.1186/s12879-022-07678-8 |
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