Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520
<i>Streptomyces</i> sp. SCSIO ZS0520 is a deep-sea hydrothermal vent-derived actinomycete. Our previous metabolism investigation showed that <i>Streptomyces</i> sp. SCSIO ZS0520 is a producer of cytotoxic actinopyrones. Here, another four types of secondary metabolites were i...
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MDPI AG
2022-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/6/393 |
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| author | Huaran Zhang Yingying Chen Yanqing Li Yongxiang Song Junying Ma Jianhua Ju |
| author_facet | Huaran Zhang Yingying Chen Yanqing Li Yongxiang Song Junying Ma Jianhua Ju |
| author_sort | Huaran Zhang |
| collection | DOAJ |
| description | <i>Streptomyces</i> sp. SCSIO ZS0520 is a deep-sea hydrothermal vent-derived actinomycete. Our previous metabolism investigation showed that <i>Streptomyces</i> sp. SCSIO ZS0520 is a producer of cytotoxic actinopyrones. Here, another four types of secondary metabolites were identified, including six salinomycin isomers (<b>2</b>–<b>7</b>), the macrolide elaiophylin (<b>8</b>), the triterpene <i>N</i>-acetyl-aminobacteriohopanetriol (<b>9</b>), and the pyrone minipyrone (<b>10</b>). Among them, compounds <b>2</b>–<b>6</b> and <b>10</b> are new compounds. To understand the biosynthetic pathway of these compounds, a bioinformatic analysis of the whole genome was carried out, which identified 34 secondary metabolite biosynthetic gene clusters. Next, the biosynthetic pathways responsive to four types of products were deduced on the basis of gene function predictions and structure information. Taken together, these findings prove the metabolite potential of ZS0520 and lay the foundations to solve the remaining biosynthetic issues in four types of marine natural products. |
| first_indexed | 2024-03-09T23:12:27Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T23:12:27Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-26246d01b2c346b99052e9330995b6cc2023-11-23T17:41:25ZengMDPI AGMarine Drugs1660-33972022-06-0120639310.3390/md20060393Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520Huaran Zhang0Yingying Chen1Yanqing Li2Yongxiang Song3Junying Ma4Jianhua Ju5Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No.1119, Haibin Road, Nansha District, Guangzhou 511458, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No.1119, Haibin Road, Nansha District, Guangzhou 511458, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No.1119, Haibin Road, Nansha District, Guangzhou 511458, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No.1119, Haibin Road, Nansha District, Guangzhou 511458, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No.1119, Haibin Road, Nansha District, Guangzhou 511458, ChinaSouthern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No.1119, Haibin Road, Nansha District, Guangzhou 511458, China<i>Streptomyces</i> sp. SCSIO ZS0520 is a deep-sea hydrothermal vent-derived actinomycete. Our previous metabolism investigation showed that <i>Streptomyces</i> sp. SCSIO ZS0520 is a producer of cytotoxic actinopyrones. Here, another four types of secondary metabolites were identified, including six salinomycin isomers (<b>2</b>–<b>7</b>), the macrolide elaiophylin (<b>8</b>), the triterpene <i>N</i>-acetyl-aminobacteriohopanetriol (<b>9</b>), and the pyrone minipyrone (<b>10</b>). Among them, compounds <b>2</b>–<b>6</b> and <b>10</b> are new compounds. To understand the biosynthetic pathway of these compounds, a bioinformatic analysis of the whole genome was carried out, which identified 34 secondary metabolite biosynthetic gene clusters. Next, the biosynthetic pathways responsive to four types of products were deduced on the basis of gene function predictions and structure information. Taken together, these findings prove the metabolite potential of ZS0520 and lay the foundations to solve the remaining biosynthetic issues in four types of marine natural products.https://www.mdpi.com/1660-3397/20/6/393polyketidebiosynthesis<i>Streptomyces</i>hydrothermal ventdeep sea |
| spellingShingle | Huaran Zhang Yingying Chen Yanqing Li Yongxiang Song Junying Ma Jianhua Ju Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520 Marine Drugs polyketide biosynthesis <i>Streptomyces</i> hydrothermal vent deep sea |
| title | Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520 |
| title_full | Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520 |
| title_fullStr | Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520 |
| title_full_unstemmed | Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520 |
| title_short | Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived <i>Streptomyces</i> sp. SCSIO ZS0520 |
| title_sort | secondary metabolites and biosynthetic gene clusters analysis of deep sea hydrothermal vent derived i streptomyces i sp scsio zs0520 |
| topic | polyketide biosynthesis <i>Streptomyces</i> hydrothermal vent deep sea |
| url | https://www.mdpi.com/1660-3397/20/6/393 |
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