Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran
Objective: To determine the antimicrobial susceptibility patterns, the frequency of integrons and associated gene cassettes in Acinetobacter baumannii (A. baumannii) strains isolated from selected hospital intensive care units. Methods: During a ten-month period, 120 A. baumannii isolates were st...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-09-01
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| Series: | Journal of Acute Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S222161891630110X |
| _version_ | 1818509398379593728 |
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| author | Hossein Goudarzi Mehdi Azad Sima Sadat Seyedjavadi Hadi Azimi Alireza Salimi Chirani Vahid Fallah Omrani Mehdi Goudarzi |
| author_facet | Hossein Goudarzi Mehdi Azad Sima Sadat Seyedjavadi Hadi Azimi Alireza Salimi Chirani Vahid Fallah Omrani Mehdi Goudarzi |
| author_sort | Hossein Goudarzi |
| collection | DOAJ |
| description | Objective: To determine the antimicrobial susceptibility patterns, the frequency of
integrons and associated gene cassettes in Acinetobacter baumannii (A. baumannii)
strains isolated from selected hospital intensive care units.
Methods: During a ten-month period, 120 A. baumannii isolates were studied. The
resistance rates to different classes of antimicrobial agents were determined. PCR was
used to detect different types of integrons and associated gene cassettes.
Results: The resistance rates to the majority of antibiotics tested were found to be between 39.3% and 99.1%. No isolate was observed to be resistant to colistin and polymyxin B. The rate of extensive drug-resistance among these clinical isolates was 62.5%.
The prevalence of class 1 and 2 integrons was found to be 74.1% and 12.5%, respectively. Seven different gene cassettes (ampC, aacA4-catB8, ISAba1-blaOXA-23-GES-14,
aadA2-cm1A6-GES-14-qacF, VIM-25-GES-24-qacF, dfrA5-ISAba1-blaOXA-51-blaOXA-40
and aadA2-GES-11-IMP-1) were observed in Class 1 integron-carrying strains. Three
gene cassettes (IMP-4, VIM-2-VEB-aacA4 and dfrA2-sat-2-aadA4) were detected in class
2 integron-bearing A. baumannii strains.
Conclusions: A high prevalence of integron was described among multidrug resistant
A. baumannii in the hospital. The findings highlighted the need for continuous surveillance in
order to prevent dissemination of multidrug resistance among A. baumannii strains in Iran. |
| first_indexed | 2024-12-10T22:44:59Z |
| format | Article |
| id | doaj.art-2624c78454a74f42b4d6a61564ac9c08 |
| institution | Directory Open Access Journal |
| issn | 2221-6189 |
| language | English |
| last_indexed | 2024-12-10T22:44:59Z |
| publishDate | 2016-09-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-2624c78454a74f42b4d6a61564ac9c082022-12-22T01:30:36ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892016-09-015538639210.1016/j.joad.2016.08.004Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, IranHossein Goudarzi0Mehdi Azad1Sima Sadat Seyedjavadi2Hadi Azimi3Alireza Salimi Chirani4Vahid Fallah Omrani5Mehdi Goudarzi6Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Laboratory Sciences, School of Paramedicine, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Medical Mycology, Pasteur Institute of Iran, Tehran, IranDepartment of English Language Teaching, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranCellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranObjective: To determine the antimicrobial susceptibility patterns, the frequency of integrons and associated gene cassettes in Acinetobacter baumannii (A. baumannii) strains isolated from selected hospital intensive care units. Methods: During a ten-month period, 120 A. baumannii isolates were studied. The resistance rates to different classes of antimicrobial agents were determined. PCR was used to detect different types of integrons and associated gene cassettes. Results: The resistance rates to the majority of antibiotics tested were found to be between 39.3% and 99.1%. No isolate was observed to be resistant to colistin and polymyxin B. The rate of extensive drug-resistance among these clinical isolates was 62.5%. The prevalence of class 1 and 2 integrons was found to be 74.1% and 12.5%, respectively. Seven different gene cassettes (ampC, aacA4-catB8, ISAba1-blaOXA-23-GES-14, aadA2-cm1A6-GES-14-qacF, VIM-25-GES-24-qacF, dfrA5-ISAba1-blaOXA-51-blaOXA-40 and aadA2-GES-11-IMP-1) were observed in Class 1 integron-carrying strains. Three gene cassettes (IMP-4, VIM-2-VEB-aacA4 and dfrA2-sat-2-aadA4) were detected in class 2 integron-bearing A. baumannii strains. Conclusions: A high prevalence of integron was described among multidrug resistant A. baumannii in the hospital. The findings highlighted the need for continuous surveillance in order to prevent dissemination of multidrug resistance among A. baumannii strains in Iran.http://www.sciencedirect.com/science/article/pii/S222161891630110XAcinetobacter baumanniiMultidrug resistanceIntegronIntensive care unit |
| spellingShingle | Hossein Goudarzi Mehdi Azad Sima Sadat Seyedjavadi Hadi Azimi Alireza Salimi Chirani Vahid Fallah Omrani Mehdi Goudarzi Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran Journal of Acute Disease Acinetobacter baumannii Multidrug resistance Integron Intensive care unit |
| title | Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran |
| title_full | Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran |
| title_fullStr | Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran |
| title_full_unstemmed | Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran |
| title_short | Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran |
| title_sort | characteriz ation of integrons and associated gene cassettes in acinetobacter baumannii strains isolated from intensive care unit in tehran iran |
| topic | Acinetobacter baumannii Multidrug resistance Integron Intensive care unit |
| url | http://www.sciencedirect.com/science/article/pii/S222161891630110X |
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