Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase
The most common decellularization method involves lipid removal using surfactant sodium dodecyl sulfate (SDS) and DNA fragmentation using DNase, and is associated with residual SDS. We previously proposed a decellularization method for the porcine aorta and ostrich carotid artery using liquefied dim...
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2023-04-01
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| author | Hideki Kanda Kento Oya Wahyudiono Motonobu Goto |
| author_facet | Hideki Kanda Kento Oya Wahyudiono Motonobu Goto |
| author_sort | Hideki Kanda |
| collection | DOAJ |
| description | The most common decellularization method involves lipid removal using surfactant sodium dodecyl sulfate (SDS) and DNA fragmentation using DNase, and is associated with residual SDS. We previously proposed a decellularization method for the porcine aorta and ostrich carotid artery using liquefied dimethyl ether (DME), which is free from the concerns associated with SDS residues, instead of SDS. In this study, the DME + DNase method was tested on crushed porcine auricular cartilage tissues. Unlike with the porcine aorta and the ostrich carotid artery, it is important to degas the porcine auricular cartilage using an aspirator before DNA fragmentation. Although approximately 90% of the lipids were removed using this method, approximately 2/3 of the water was removed, resulting in a temporary Schiff base reaction. The amount of residual DNA in the tissue was approximately 27 ng/mg dry weight, which is lower than the regulatory value of 50 ng/mg dry weight. Hematoxylin and eosin staining confirmed that cell nuclei were removed from the tissue. Residual DNA fragment length assessment by electrophoresis confirmed that the residual DNA was fragmented to less than 100 bp, which was lower than the regulatory limit of 200 bp. By contrast, in the uncrushed sample, only the surface was decellularized. Thus, although limited to a sample size of approximately 1 mm, liquefied DME can be used to decellularize porcine auricular cartilage. Thus, liquefied DME, with its low persistence and high lipid removal capacity, is an effective alternative to SDS. |
| first_indexed | 2024-03-11T04:47:21Z |
| format | Article |
| id | doaj.art-2626133232fa4f75b933b0dc8448f678 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T04:47:21Z |
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| spelling | doaj.art-2626133232fa4f75b933b0dc8448f6782023-11-17T20:14:11ZengMDPI AGMaterials1996-19442023-04-01168317210.3390/ma16083172Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNaseHideki Kanda0Kento Oya1Wahyudiono2Motonobu Goto3Department of Materials Process Engineering, Nagoya University, Nagoya 464-8603, JapanDepartment of Materials Process Engineering, Nagoya University, Nagoya 464-8603, JapanDepartment of Materials Process Engineering, Nagoya University, Nagoya 464-8603, JapanDepartment of Materials Process Engineering, Nagoya University, Nagoya 464-8603, JapanThe most common decellularization method involves lipid removal using surfactant sodium dodecyl sulfate (SDS) and DNA fragmentation using DNase, and is associated with residual SDS. We previously proposed a decellularization method for the porcine aorta and ostrich carotid artery using liquefied dimethyl ether (DME), which is free from the concerns associated with SDS residues, instead of SDS. In this study, the DME + DNase method was tested on crushed porcine auricular cartilage tissues. Unlike with the porcine aorta and the ostrich carotid artery, it is important to degas the porcine auricular cartilage using an aspirator before DNA fragmentation. Although approximately 90% of the lipids were removed using this method, approximately 2/3 of the water was removed, resulting in a temporary Schiff base reaction. The amount of residual DNA in the tissue was approximately 27 ng/mg dry weight, which is lower than the regulatory value of 50 ng/mg dry weight. Hematoxylin and eosin staining confirmed that cell nuclei were removed from the tissue. Residual DNA fragment length assessment by electrophoresis confirmed that the residual DNA was fragmented to less than 100 bp, which was lower than the regulatory limit of 200 bp. By contrast, in the uncrushed sample, only the surface was decellularized. Thus, although limited to a sample size of approximately 1 mm, liquefied DME can be used to decellularize porcine auricular cartilage. Thus, liquefied DME, with its low persistence and high lipid removal capacity, is an effective alternative to SDS.https://www.mdpi.com/1996-1944/16/8/3172decellularizationextractionliquefied gasscaffoldsubcritical fluid |
| spellingShingle | Hideki Kanda Kento Oya Wahyudiono Motonobu Goto Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase Materials decellularization extraction liquefied gas scaffold subcritical fluid |
| title | Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase |
| title_full | Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase |
| title_fullStr | Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase |
| title_full_unstemmed | Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase |
| title_short | Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase |
| title_sort | surfactant free decellularization of porcine auricular cartilage using liquefied dimethyl ether and dnase |
| topic | decellularization extraction liquefied gas scaffold subcritical fluid |
| url | https://www.mdpi.com/1996-1944/16/8/3172 |
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