Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitors
Human norovirus (HuNoV) is an important enteric virus that can cause large gastroenteritis outbreaks via the fecal-oral route from contaminated water and produce. Real-time quantitative reverse transcription PCR (RT-qPCR) is the only method to apply the routine detection of HuNoV in various samples,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021016637 |
| _version_ | 1819299392001146880 |
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| author | SungJun Park Cheonghoon Lee Kyuseon Cho Hye Young Ko Sung Jae Jang GwangPyo Ko |
| author_facet | SungJun Park Cheonghoon Lee Kyuseon Cho Hye Young Ko Sung Jae Jang GwangPyo Ko |
| author_sort | SungJun Park |
| collection | DOAJ |
| description | Human norovirus (HuNoV) is an important enteric virus that can cause large gastroenteritis outbreaks via the fecal-oral route from contaminated water and produce. Real-time quantitative reverse transcription PCR (RT-qPCR) is the only method to apply the routine detection of HuNoV in various samples, however, inhibitors present in the samples can affect the accuracy and sensitivity of RT-qPCR results. Here, we suggest an inhibitor-removal treatment for two types of noroviruses using two commercial kits. Two types of water sample (surface and seawater) and four types of produce (green onions, lettuces, radishes, and strawberries) were evaluated. The recovery efficiencies of noroviruses in water samples clearly increased in surface and seawater samples with the inhibitor-removal treatment compared to untreated samples. Moreover, murine norovirus-1 was well recovered from the four types of produce with the inhibitor-removal treatment. The mean recovery efficiencies of HuNoV genogroup II genotype 4 in lettuces and strawberries were also increased in the treated samples. Therefore, we suggest that the inhibitor-removal treatment could be useful for improving the accuracy and sensitivity of RT-qPCR methods for noroviruses in water and produce. |
| first_indexed | 2024-12-24T05:45:03Z |
| format | Article |
| id | doaj.art-2626dd97d9594440bc33896267f2a8e6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-24T05:45:03Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-2626dd97d9594440bc33896267f2a8e62022-12-21T17:12:37ZengElsevierHeliyon2405-84402021-07-0177e07560Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitorsSungJun Park0Cheonghoon Lee1Kyuseon Cho2Hye Young Ko3Sung Jae Jang4GwangPyo Ko5Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea; N-Bio, Seoul National University, Seoul, Republic of KoreaDepartment of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea; Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea; Corresponding author.Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of KoreaDepartment of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of KoreaDepartment of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of KoreaDepartment of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea; N-Bio, Seoul National University, Seoul, Republic of Korea; Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea; Corresponding author.Human norovirus (HuNoV) is an important enteric virus that can cause large gastroenteritis outbreaks via the fecal-oral route from contaminated water and produce. Real-time quantitative reverse transcription PCR (RT-qPCR) is the only method to apply the routine detection of HuNoV in various samples, however, inhibitors present in the samples can affect the accuracy and sensitivity of RT-qPCR results. Here, we suggest an inhibitor-removal treatment for two types of noroviruses using two commercial kits. Two types of water sample (surface and seawater) and four types of produce (green onions, lettuces, radishes, and strawberries) were evaluated. The recovery efficiencies of noroviruses in water samples clearly increased in surface and seawater samples with the inhibitor-removal treatment compared to untreated samples. Moreover, murine norovirus-1 was well recovered from the four types of produce with the inhibitor-removal treatment. The mean recovery efficiencies of HuNoV genogroup II genotype 4 in lettuces and strawberries were also increased in the treated samples. Therefore, we suggest that the inhibitor-removal treatment could be useful for improving the accuracy and sensitivity of RT-qPCR methods for noroviruses in water and produce.http://www.sciencedirect.com/science/article/pii/S2405844021016637NorovirusReal-time quantitative reverse transcription PCRInhibitorWaterVegetableBerry |
| spellingShingle | SungJun Park Cheonghoon Lee Kyuseon Cho Hye Young Ko Sung Jae Jang GwangPyo Ko Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitors Heliyon Norovirus Real-time quantitative reverse transcription PCR Inhibitor Water Vegetable Berry |
| title | Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitors |
| title_full | Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitors |
| title_fullStr | Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitors |
| title_full_unstemmed | Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitors |
| title_short | Improved real-time quantitative reverse transcription PCR detection of norovirus following removal of inhibitors |
| title_sort | improved real time quantitative reverse transcription pcr detection of norovirus following removal of inhibitors |
| topic | Norovirus Real-time quantitative reverse transcription PCR Inhibitor Water Vegetable Berry |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021016637 |
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