Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro
Objective(s): A growing body of evidence indicates that rhomboid domain containing 1 (RHBDD1) plays an important role in a variety of physiological and pathological processes, including tumorigenesis. We aimed to determine the function of RHBDD1 in breast cancer cells. Materials and Methods: In this...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2018-11-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_11549_e4fa6d97b814cb26189ba482a298b866.pdf |
| _version_ | 1818380523440963584 |
|---|---|
| author | Chunjun Huang Xiaochun Ji Yinyin Peng Minghua Wu Weizhu Wu Yong Luo Gaoxiang Cheng Ye Zhu |
| author_facet | Chunjun Huang Xiaochun Ji Yinyin Peng Minghua Wu Weizhu Wu Yong Luo Gaoxiang Cheng Ye Zhu |
| author_sort | Chunjun Huang |
| collection | DOAJ |
| description | Objective(s): A growing body of evidence indicates that rhomboid domain containing 1 (RHBDD1) plays an important role in a variety of physiological and pathological processes, including tumorigenesis. We aimed to determine the function of RHBDD1 in breast cancer cells. Materials and Methods: In this study, we used the Oncomine™ database to determine the expression patterns of RHBDD1 in normal and breast cancer tissues. We performed lentiviral transfection of RHBDD1-specific small interfering RNA into the breast cancer cell lines ZR-75-30 and MDA-MB-231 in order to investigate the effects of RHBDD1 deficiency on breast cancer metastasis. Results: We found that knockdown of RHBDD1 inhibited breast cancer cell migration and invasion in vitro. Moreover, knockdown of RHBDD1 promoted epithelial–mesenchymal transition (EMT) by suppressing the expression of MPP2, MPP9, fibronectin 1, vimentin, SRY-box 2, zinc finger E-box binding homeobox 1, and snail family transcriptional repressor 1, and promoting the expression of cadherin 1. Additionally, knockdown of RHBDD1 inhibited the protein expression and phosphorylation of Akt.Conclusion: Our data indicate that RHBDD1 overexpression may promote breast cancer metastasis via the regulation of EMT, suggesting that RHBDD1 may be an important regulator of breast cancer metastasis. |
| first_indexed | 2024-12-14T02:20:02Z |
| format | Article |
| id | doaj.art-2626e7457f7e430f93eea7305b19e929 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-14T02:20:02Z |
| publishDate | 2018-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-2626e7457f7e430f93eea7305b19e9292022-12-21T23:20:32ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-11-0121111161116610.22038/ijbms.2018.29788.719111549Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitroChunjun Huang0Xiaochun Ji1Yinyin Peng2Minghua Wu3Weizhu Wu4Yong Luo5Gaoxiang Cheng6Ye Zhu7Thyroid Breast Surgery, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, 315000, ChinaThyroid Breast Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo, 315000, ChinaThyroid Breast Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo, 315000, ChinaThyroid Breast Surgery, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, 315000, ChinaThyroid Breast Surgery, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, 315000, ChinaThyroid Breast Surgery, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, 315000, ChinaThyroid Breast Surgery, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, 315000, ChinaThyroid Breast Surgery, Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, 315000, ChinaObjective(s): A growing body of evidence indicates that rhomboid domain containing 1 (RHBDD1) plays an important role in a variety of physiological and pathological processes, including tumorigenesis. We aimed to determine the function of RHBDD1 in breast cancer cells. Materials and Methods: In this study, we used the Oncomine™ database to determine the expression patterns of RHBDD1 in normal and breast cancer tissues. We performed lentiviral transfection of RHBDD1-specific small interfering RNA into the breast cancer cell lines ZR-75-30 and MDA-MB-231 in order to investigate the effects of RHBDD1 deficiency on breast cancer metastasis. Results: We found that knockdown of RHBDD1 inhibited breast cancer cell migration and invasion in vitro. Moreover, knockdown of RHBDD1 promoted epithelial–mesenchymal transition (EMT) by suppressing the expression of MPP2, MPP9, fibronectin 1, vimentin, SRY-box 2, zinc finger E-box binding homeobox 1, and snail family transcriptional repressor 1, and promoting the expression of cadherin 1. Additionally, knockdown of RHBDD1 inhibited the protein expression and phosphorylation of Akt.Conclusion: Our data indicate that RHBDD1 overexpression may promote breast cancer metastasis via the regulation of EMT, suggesting that RHBDD1 may be an important regulator of breast cancer metastasis.http://ijbms.mums.ac.ir/article_11549_e4fa6d97b814cb26189ba482a298b866.pdfBreast neoplasmsLentivirusNeoplasm metastasisRhomboid domain containing 1RNASmall interfering |
| spellingShingle | Chunjun Huang Xiaochun Ji Yinyin Peng Minghua Wu Weizhu Wu Yong Luo Gaoxiang Cheng Ye Zhu Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro Iranian Journal of Basic Medical Sciences Breast neoplasms Lentivirus Neoplasm metastasis Rhomboid domain containing 1 RNA Small interfering |
| title | Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro |
| title_full | Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro |
| title_fullStr | Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro |
| title_full_unstemmed | Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro |
| title_short | Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro |
| title_sort | silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro |
| topic | Breast neoplasms Lentivirus Neoplasm metastasis Rhomboid domain containing 1 RNA Small interfering |
| url | http://ijbms.mums.ac.ir/article_11549_e4fa6d97b814cb26189ba482a298b866.pdf |
| work_keys_str_mv | AT chunjunhuang silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro AT xiaochunji silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro AT yinyinpeng silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro AT minghuawu silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro AT weizhuwu silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro AT yongluo silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro AT gaoxiangcheng silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro AT yezhu silencingofrhomboiddomaincontaining1toinhibitthemetastasisofhumanbreastcancercellsinvitro |