Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in Sheep
Yeast products (YP) are commonly used as rumen regulators, but their mechanisms of action are still unclear. Based on our previous studies, we questioned whether yeast products would have an impact on rumen solid-associated (SA) and liquid-associated (LA) microorganisms and alter rumen fermentation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/22/3221 |
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| author | Manchun Su Huihui Wang Huibin Shi Qiao Li Yong Zhang Taotao Li Youji Ma |
| author_facet | Manchun Su Huihui Wang Huibin Shi Qiao Li Yong Zhang Taotao Li Youji Ma |
| author_sort | Manchun Su |
| collection | DOAJ |
| description | Yeast products (YP) are commonly used as rumen regulators, but their mechanisms of action are still unclear. Based on our previous studies, we questioned whether yeast products would have an impact on rumen solid-associated (SA) and liquid-associated (LA) microorganisms and alter rumen fermentation patterns. Thirty 3-month-old male sheep weighing 19.27 ± 0.45 kg were selected and randomized into three groups for 60 days: (1) basal diet group (CON group), (2) basal diet add 20 g YP per day (low YP, LYP group) and (3) basal diet add 40 g YP per day (high YP, HYP group). The results demonstrated that the addition of YP increased rumen cellulase activity, butyrate and total volatile fatty acid (TVFA) concentrations (<i>p</i> < 0.05), while it decreased rumen amylase activity and abnormal metabolites, such as lactate, lipopolysaccharides (LPS) and histamine (HIS) (<i>p</i> < 0.05). Metagenomic analysis of rumen microorganisms in three groups revealed that YP mainly influenced the microbial profiles of the SA system. YP increased the relative abundance of <i>R. flavefaciens</i> and decreased methanogens in the SA system (<i>p</i> < 0.05). With the addition of YP, the abundance of only a few lactate-producing bacteria increased in the SA system, including <i>Streptococcus</i> and <i>Lactobacillus</i> (<i>p</i> < 0.05). However, almost all lactate-utilizing bacteria increased in the LA system, including <i>Megasphaera</i>, <i>Selenomonas</i>, <i>Fusobacterium</i> and <i>Veillonella</i> (<i>p</i> < 0.05). In addition, YP increased the abundance of certain GHs family members, including GH43 and GH98 (<i>p</i> < 0.05), but decreased the abundance of some KEGG metabolic pathways involved in starch and sucrose metabolism, biosynthesis of antibiotics and purine metabolism, among others. In conclusion, the addition of YP to high-concentrate diets can change the abundance of major functional microbiota in the rumen, especially in the solid fraction, which in turn affects rumen fermentation patterns and improves rumen digestibility. |
| first_indexed | 2024-03-09T18:30:58Z |
| format | Article |
| id | doaj.art-262765d4fdbc413aa2f2ae03987a1132 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T18:30:58Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-262765d4fdbc413aa2f2ae03987a11322023-11-24T07:30:11ZengMDPI AGAnimals2076-26152022-11-011222322110.3390/ani12223221Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in SheepManchun Su0Huihui Wang1Huibin Shi2Qiao Li3Yong Zhang4Taotao Li5Youji Ma6College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaYeast products (YP) are commonly used as rumen regulators, but their mechanisms of action are still unclear. Based on our previous studies, we questioned whether yeast products would have an impact on rumen solid-associated (SA) and liquid-associated (LA) microorganisms and alter rumen fermentation patterns. Thirty 3-month-old male sheep weighing 19.27 ± 0.45 kg were selected and randomized into three groups for 60 days: (1) basal diet group (CON group), (2) basal diet add 20 g YP per day (low YP, LYP group) and (3) basal diet add 40 g YP per day (high YP, HYP group). The results demonstrated that the addition of YP increased rumen cellulase activity, butyrate and total volatile fatty acid (TVFA) concentrations (<i>p</i> < 0.05), while it decreased rumen amylase activity and abnormal metabolites, such as lactate, lipopolysaccharides (LPS) and histamine (HIS) (<i>p</i> < 0.05). Metagenomic analysis of rumen microorganisms in three groups revealed that YP mainly influenced the microbial profiles of the SA system. YP increased the relative abundance of <i>R. flavefaciens</i> and decreased methanogens in the SA system (<i>p</i> < 0.05). With the addition of YP, the abundance of only a few lactate-producing bacteria increased in the SA system, including <i>Streptococcus</i> and <i>Lactobacillus</i> (<i>p</i> < 0.05). However, almost all lactate-utilizing bacteria increased in the LA system, including <i>Megasphaera</i>, <i>Selenomonas</i>, <i>Fusobacterium</i> and <i>Veillonella</i> (<i>p</i> < 0.05). In addition, YP increased the abundance of certain GHs family members, including GH43 and GH98 (<i>p</i> < 0.05), but decreased the abundance of some KEGG metabolic pathways involved in starch and sucrose metabolism, biosynthesis of antibiotics and purine metabolism, among others. In conclusion, the addition of YP to high-concentrate diets can change the abundance of major functional microbiota in the rumen, especially in the solid fraction, which in turn affects rumen fermentation patterns and improves rumen digestibility.https://www.mdpi.com/2076-2615/12/22/3221feed supplementruminal digestive systemsubenvironmental microbiotasmall ruminants |
| spellingShingle | Manchun Su Huihui Wang Huibin Shi Qiao Li Yong Zhang Taotao Li Youji Ma Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in Sheep Animals feed supplement ruminal digestive system subenvironmental microbiota small ruminants |
| title | Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in Sheep |
| title_full | Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in Sheep |
| title_fullStr | Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in Sheep |
| title_full_unstemmed | Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in Sheep |
| title_short | Yeast Products Mediated Ruminal Subenvironmental Microbiota, and Abnormal Metabolites and Digestive Enzymes Regulated Rumen Fermentation Function in Sheep |
| title_sort | yeast products mediated ruminal subenvironmental microbiota and abnormal metabolites and digestive enzymes regulated rumen fermentation function in sheep |
| topic | feed supplement ruminal digestive system subenvironmental microbiota small ruminants |
| url | https://www.mdpi.com/2076-2615/12/22/3221 |
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