Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005
Bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. The antimicrobial susceptibility patterns of 1897 gram-positive bacterial Isolates were evaluated. The minimum inhibitory concentration (MIC) of isolates which comprised Staphyloc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2009-08-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/3601 |
| _version_ | 1818758739946110976 |
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| author | Marzieh Aligholi Mohammad Emaneini Fereshteh Jabalameli Shadi Shahsavan Zohreh Abdolmaleki Hossein Sedaghat Nematollah Jonaidi |
| author_facet | Marzieh Aligholi Mohammad Emaneini Fereshteh Jabalameli Shadi Shahsavan Zohreh Abdolmaleki Hossein Sedaghat Nematollah Jonaidi |
| author_sort | Marzieh Aligholi |
| collection | DOAJ |
| description | Bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. The antimicrobial susceptibility patterns of 1897 gram-positive bacterial Isolates were evaluated. The minimum inhibitory concentration (MIC) of isolates which comprised Staphylococcus aureus (927 isolates), coagulase-negative staphylococci (CNS; 425 isolates), Enterococcus faecalis (320 isolates), Enterococcus faecium (157 isolates), and pneumococci (50 isolates) collected from 3 teaching hospitals in Tehran were determined by agar dilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. The presence of mecA gene was investigated in methicillin-resistant staphylococci by PCR method and vanA and vanB genes were targeted in enterococcal isolates by Multiplex PCR method. The resistance rate to methicillin among S. aureus and CNS isolates were 33% and 49%, respectively. All S. aureus isolates were susceptible to vancomycin .The lowest rate of resistance in all S. aureus isolates was found for rifampicin (<4%). The vancomycin resistance rate in enterococci isolates was 11% which was more frequent among E. faecium (19%) than E. faecalis (4%), all resistant isolates carrying vanA. High-level resistance to gentamicin and streptomycin, were detected in 47% and 87% of enterococcal isolates respectively. The rate of penicillin resistance in pneumococci was 3% and about 27% of isolates had reduced susceptibility to penicillin. The prevalence of erythromycin resistant among pneumococci was 58%. All pneumococcal isolates were susceptible to ceftriaxone, rifampicin and vancomycin. Our data highlight the importance of access to updated bacterial susceptibility data regarding commonly prescribed agents for clinicians in Iran. |
| first_indexed | 2024-12-18T06:31:38Z |
| format | Article |
| id | doaj.art-262c9d3c79774d5a985e86ffa8cfdbce |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-18T06:31:38Z |
| publishDate | 2009-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-262c9d3c79774d5a985e86ffa8cfdbce2022-12-21T21:17:54ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942009-08-01474Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005Marzieh Aligholi0Mohammad Emaneini1Fereshteh Jabalameli2Shadi Shahsavan3Zohreh Abdolmaleki4Hossein Sedaghat5Nematollah Jonaidi6Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Veterinary Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Health Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.Bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. The antimicrobial susceptibility patterns of 1897 gram-positive bacterial Isolates were evaluated. The minimum inhibitory concentration (MIC) of isolates which comprised Staphylococcus aureus (927 isolates), coagulase-negative staphylococci (CNS; 425 isolates), Enterococcus faecalis (320 isolates), Enterococcus faecium (157 isolates), and pneumococci (50 isolates) collected from 3 teaching hospitals in Tehran were determined by agar dilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. The presence of mecA gene was investigated in methicillin-resistant staphylococci by PCR method and vanA and vanB genes were targeted in enterococcal isolates by Multiplex PCR method. The resistance rate to methicillin among S. aureus and CNS isolates were 33% and 49%, respectively. All S. aureus isolates were susceptible to vancomycin .The lowest rate of resistance in all S. aureus isolates was found for rifampicin (<4%). The vancomycin resistance rate in enterococci isolates was 11% which was more frequent among E. faecium (19%) than E. faecalis (4%), all resistant isolates carrying vanA. High-level resistance to gentamicin and streptomycin, were detected in 47% and 87% of enterococcal isolates respectively. The rate of penicillin resistance in pneumococci was 3% and about 27% of isolates had reduced susceptibility to penicillin. The prevalence of erythromycin resistant among pneumococci was 58%. All pneumococcal isolates were susceptible to ceftriaxone, rifampicin and vancomycin. Our data highlight the importance of access to updated bacterial susceptibility data regarding commonly prescribed agents for clinicians in Iran.https://acta.tums.ac.ir/index.php/acta/article/view/3601Antimicrobial susceptibility patternsgram-positive CocciVREPneumococci |
| spellingShingle | Marzieh Aligholi Mohammad Emaneini Fereshteh Jabalameli Shadi Shahsavan Zohreh Abdolmaleki Hossein Sedaghat Nematollah Jonaidi Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005 Acta Medica Iranica Antimicrobial susceptibility patterns gram-positive Cocci VRE Pneumococci |
| title | Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005 |
| title_full | Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005 |
| title_fullStr | Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005 |
| title_full_unstemmed | Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005 |
| title_short | Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005 |
| title_sort | antibiotic susceptibility pattern of gram positive cocci cultured from patients in three university hospitals in tehran iran during 2001 2005 |
| topic | Antimicrobial susceptibility patterns gram-positive Cocci VRE Pneumococci |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/3601 |
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