Fluopyram activates systemic resistance in soybean
The soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is a significant yield-limiting factor in soybean production in the Midwestern US. Several management practices are implemented to mitigate yield losses caused by SCN, including using SDHI (succinate dehydrogenase inhibitors) fungicides...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1020167/full |
| _version_ | 1797990775237115904 |
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| author | Leonardo F. Rocha Arjun Subedi Mirian F. Pimentel Jason P. Bond Ahmad M. Fakhoury |
| author_facet | Leonardo F. Rocha Arjun Subedi Mirian F. Pimentel Jason P. Bond Ahmad M. Fakhoury |
| author_sort | Leonardo F. Rocha |
| collection | DOAJ |
| description | The soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is a significant yield-limiting factor in soybean production in the Midwestern US. Several management practices are implemented to mitigate yield losses caused by SCN, including using SDHI (succinate dehydrogenase inhibitors) fungicides delivered as seed treatments. A set of studies was conducted to evaluate the effect of two seed-applied succinate dehydrogenase inhibitors (SDHI) compounds, fluopyram and pydiflumetofen, on SCN population densities, plant injury, and plant growth. Cyst counts in untreated control and pydiflumetofen treated plants were 3.44 and 3.59 times higher than fluopyram, respectively, while egg counts were 8.25 and 7.06 times higher in control and pydiflumetofen. Next-generation sequencing was later employed to identify transcriptomic shifts in gene expression profiles in fluopyram and pydiflumetofen -treated seedlings. RNA expression patterns of seed treatments clustered by sampling time (5 DAP vs. 10 DAP); therefore, downstream analysis was conducted by timepoint. At 5 DAP, 10,870 and 325 differentially expressed genes (DEG) were identified in fluopyram and pydiflumetofen, respectively. These same treatments generated 219 and 2 DEGs at 10 DAP. Multiple DEGs identified in soybean seedlings treated with fluopyram are linked to systemic resistance, suggesting a potential role of systemic resistance in the suppression of SCN by fluopyram, in addition to the known nematicidal activity. The non-target inhibition of soybean succinate dehydrogenase genes by fluopyram may be the origin of the phytotoxicity symptoms observed and potentially the source of the systemic resistance activation reported in the current study. This work helps to elucidate the mechanisms of suppression of SCN by fluopyram. |
| first_indexed | 2024-04-11T08:41:45Z |
| format | Article |
| id | doaj.art-263764968c414dffb340f31d4c14c822 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T08:41:45Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-263764968c414dffb340f31d4c14c8222022-12-22T04:34:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10201671020167Fluopyram activates systemic resistance in soybeanLeonardo F. RochaArjun SubediMirian F. PimentelJason P. BondAhmad M. FakhouryThe soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is a significant yield-limiting factor in soybean production in the Midwestern US. Several management practices are implemented to mitigate yield losses caused by SCN, including using SDHI (succinate dehydrogenase inhibitors) fungicides delivered as seed treatments. A set of studies was conducted to evaluate the effect of two seed-applied succinate dehydrogenase inhibitors (SDHI) compounds, fluopyram and pydiflumetofen, on SCN population densities, plant injury, and plant growth. Cyst counts in untreated control and pydiflumetofen treated plants were 3.44 and 3.59 times higher than fluopyram, respectively, while egg counts were 8.25 and 7.06 times higher in control and pydiflumetofen. Next-generation sequencing was later employed to identify transcriptomic shifts in gene expression profiles in fluopyram and pydiflumetofen -treated seedlings. RNA expression patterns of seed treatments clustered by sampling time (5 DAP vs. 10 DAP); therefore, downstream analysis was conducted by timepoint. At 5 DAP, 10,870 and 325 differentially expressed genes (DEG) were identified in fluopyram and pydiflumetofen, respectively. These same treatments generated 219 and 2 DEGs at 10 DAP. Multiple DEGs identified in soybean seedlings treated with fluopyram are linked to systemic resistance, suggesting a potential role of systemic resistance in the suppression of SCN by fluopyram, in addition to the known nematicidal activity. The non-target inhibition of soybean succinate dehydrogenase genes by fluopyram may be the origin of the phytotoxicity symptoms observed and potentially the source of the systemic resistance activation reported in the current study. This work helps to elucidate the mechanisms of suppression of SCN by fluopyram.https://www.frontiersin.org/articles/10.3389/fpls.2022.1020167/fullGlycine maxplant-parasitic nematodesseed treatmentSDHI fungicidesRNA-Seqtranscriptomics |
| spellingShingle | Leonardo F. Rocha Arjun Subedi Mirian F. Pimentel Jason P. Bond Ahmad M. Fakhoury Fluopyram activates systemic resistance in soybean Frontiers in Plant Science Glycine max plant-parasitic nematodes seed treatment SDHI fungicides RNA-Seq transcriptomics |
| title | Fluopyram activates systemic resistance in soybean |
| title_full | Fluopyram activates systemic resistance in soybean |
| title_fullStr | Fluopyram activates systemic resistance in soybean |
| title_full_unstemmed | Fluopyram activates systemic resistance in soybean |
| title_short | Fluopyram activates systemic resistance in soybean |
| title_sort | fluopyram activates systemic resistance in soybean |
| topic | Glycine max plant-parasitic nematodes seed treatment SDHI fungicides RNA-Seq transcriptomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1020167/full |
| work_keys_str_mv | AT leonardofrocha fluopyramactivatessystemicresistanceinsoybean AT arjunsubedi fluopyramactivatessystemicresistanceinsoybean AT mirianfpimentel fluopyramactivatessystemicresistanceinsoybean AT jasonpbond fluopyramactivatessystemicresistanceinsoybean AT ahmadmfakhoury fluopyramactivatessystemicresistanceinsoybean |