Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino)
AbstractPak choi (Brassica rapa ssp. chinensis Makino) is a typical seed vernalization vegetable. Because premature bolting in spring causes significant economic losses, it is very important to clarify the flower formation mechanism to prevent this. Cytokinins are important plant hormones involved i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2022.2106888 |
| _version_ | 1797974111578750976 |
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| author | Yue Bao Xuan Li Xianhui Qi Xueting Wang Xianjun Feng Yinglong Chen Leiping Hou Meilan Li |
| author_facet | Yue Bao Xuan Li Xianhui Qi Xueting Wang Xianjun Feng Yinglong Chen Leiping Hou Meilan Li |
| author_sort | Yue Bao |
| collection | DOAJ |
| description | AbstractPak choi (Brassica rapa ssp. chinensis Makino) is a typical seed vernalization vegetable. Because premature bolting in spring causes significant economic losses, it is very important to clarify the flower formation mechanism to prevent this. Cytokinins are important plant hormones involved in regulating plant growth and development. To understand the relationship between cytokinin metabolism and flowering of pak choi, in this study, we determined the cytokinin trans-zeatin content in shoot apices of pak choi at different developmental stages by enzyme-linked immunosorbent assay. The results showed that cytokinin levels increased significantly after low temperature treatment at 4 °C, and continued to increase with vegetative growth after transplanting, reaching a peak at the critical period which is immediately prior to flower buds’ differentiation (S0), then decreased thereafter. The levels for low temperature treatment were consistently higher than controls. To explore the molecular mechanism underpinning the cytokinin changes, expression of homologous genes encoding cytokinin metabolic enzymes was analysed by transcriptome sequencing. The expression levels of nine genes (Bra004037, Bra023701, Bra002204, Bra014968, Bra028326, Bra028182, Bra034022, Bra009143 and Bra005869) were consistent with the changes in cytokinin content. The correlation between differentially expressed genes and cytokinin content in different developmental stages of apexes was analysed, and six closely related genes (Bra023701, Bra002204, Bra014968, Bra028182, Bra005869 and Bra009143) were identified. The results help to illuminate the molecular mechanisms controlling flowering of pak choi. |
| first_indexed | 2024-04-11T04:14:53Z |
| format | Article |
| id | doaj.art-26391de163f34b3286309b7f69661e2d |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-04-11T04:14:53Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-26391de163f34b3286309b7f69661e2d2022-12-31T18:03:16ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302022-12-0136178879710.1080/13102818.2022.2106888Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino)Yue Bao0Xuan Li1Xianhui Qi2Xueting Wang3Xianjun Feng4Yinglong Chen5Leiping Hou6Meilan Li7College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, PR ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, PR ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, PR ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, PR ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, PR ChinaThe UWA Institute of Agriculture, and School of Agriculture and Environment, The University of Western Australia, Perth, WA, AustraliaCollege of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, PR ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, PR ChinaAbstractPak choi (Brassica rapa ssp. chinensis Makino) is a typical seed vernalization vegetable. Because premature bolting in spring causes significant economic losses, it is very important to clarify the flower formation mechanism to prevent this. Cytokinins are important plant hormones involved in regulating plant growth and development. To understand the relationship between cytokinin metabolism and flowering of pak choi, in this study, we determined the cytokinin trans-zeatin content in shoot apices of pak choi at different developmental stages by enzyme-linked immunosorbent assay. The results showed that cytokinin levels increased significantly after low temperature treatment at 4 °C, and continued to increase with vegetative growth after transplanting, reaching a peak at the critical period which is immediately prior to flower buds’ differentiation (S0), then decreased thereafter. The levels for low temperature treatment were consistently higher than controls. To explore the molecular mechanism underpinning the cytokinin changes, expression of homologous genes encoding cytokinin metabolic enzymes was analysed by transcriptome sequencing. The expression levels of nine genes (Bra004037, Bra023701, Bra002204, Bra014968, Bra028326, Bra028182, Bra034022, Bra009143 and Bra005869) were consistent with the changes in cytokinin content. The correlation between differentially expressed genes and cytokinin content in different developmental stages of apexes was analysed, and six closely related genes (Bra023701, Bra002204, Bra014968, Bra028182, Bra005869 and Bra009143) were identified. The results help to illuminate the molecular mechanisms controlling flowering of pak choi.https://www.tandfonline.com/doi/10.1080/13102818.2022.2106888Pak choicytokiningeneflowering |
| spellingShingle | Yue Bao Xuan Li Xianhui Qi Xueting Wang Xianjun Feng Yinglong Chen Leiping Hou Meilan Li Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino) Biotechnology & Biotechnological Equipment Pak choi cytokinin gene flowering |
| title | Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino) |
| title_full | Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino) |
| title_fullStr | Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino) |
| title_full_unstemmed | Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino) |
| title_short | Analysis of cytokinin content and associated genes at different developmental stages in pak choi (Brassica rapa ssp. chinensis Makino) |
| title_sort | analysis of cytokinin content and associated genes at different developmental stages in pak choi brassica rapa ssp chinensis makino |
| topic | Pak choi cytokinin gene flowering |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2022.2106888 |
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