Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares
So far the intimate link between serum microRNA (miRNA) and uterine inflammation in mares is unknown. We aimed (I) to investigate expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 (II) and to measure concentrations of interleukin 6 (IL-6), and prostaglandins (...
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MDPI AG
2021-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/8/6/98 |
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| author | Sally Ibrahim Mohamed Hedia Mohamed O. Taqi Mohamed K. Derbala Karima Gh. M. Mahmoud Youssef Ahmed Sayed Ismail Mohamed El-Belely |
| author_facet | Sally Ibrahim Mohamed Hedia Mohamed O. Taqi Mohamed K. Derbala Karima Gh. M. Mahmoud Youssef Ahmed Sayed Ismail Mohamed El-Belely |
| author_sort | Sally Ibrahim |
| collection | DOAJ |
| description | So far the intimate link between serum microRNA (miRNA) and uterine inflammation in mares is unknown. We aimed (I) to investigate expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 (II) and to measure concentrations of interleukin 6 (IL-6), and prostaglandins (PGF<sub>2α</sub> and PGE<sub>2</sub>) in serum of mares with healthy and abnormal uterine status (endometritis). This study was conducted on 80 Arabian mares: young (4–7 years), and old (8–14 years). Mares were divided into 48 sub-fertile (endometritis) and 32 fertile (control) at stud farms. Serum was collected for measuring IL-6, PGF<sub>2α</sub>, and PGE<sub>2</sub>, as well as miRNA isolation and qRT-PCR. Concentrations of IL-6, PGE<sub>2</sub>, and PGF<sub>2α</sub> were higher in mares with endometritis compared to control. Age of mares had a remarkable effect on IL-6, PGE<sub>2</sub>, and PGF<sub>2α</sub> concentrations. Relative abundance of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 was higher in both young and old mares with endometritis. We noticed that eca-miR-155, eca-miR-223, eca-miR-200a, and eca-miR-205 revealed higher expression level in old than young mares with endometritis. This is the first study that has revealed the changes in cell free miRNA and serum inflammatory mediators during endometritis, and these findings could be used for a better understanding the pathophysiology mechanisms of endometritis in equine. |
| first_indexed | 2024-03-10T10:43:40Z |
| format | Article |
| id | doaj.art-263930ed8d264374bca13afa7199e317 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T10:43:40Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-263930ed8d264374bca13afa7199e3172023-11-21T22:44:56ZengMDPI AGVeterinary Sciences2306-73812021-06-01869810.3390/vetsci8060098Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian MaresSally Ibrahim0Mohamed Hedia1Mohamed O. Taqi2Mohamed K. Derbala3Karima Gh. M. Mahmoud4Youssef Ahmed5Sayed Ismail6Mohamed El-Belely7Department of Animal Reproduction and AI, Veterinary Research Division, National Research Centre, Dokki, Giza 12622, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptCentral Laboratory for Agricultural Climate, Agricultural Research Centre, Ministry of Agriculture and Land Reclamation, Dokki, Giza 12311, EgyptDiagnostic Imaging and Endoscopy Unit, Animal Reproduction Research Institute, Giza 12556, EgyptDepartment of Animal Reproduction and AI, Veterinary Research Division, National Research Centre, Dokki, Giza 12622, EgyptDepartment of Animal Reproduction and AI, Veterinary Research Division, National Research Centre, Dokki, Giza 12622, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptSo far the intimate link between serum microRNA (miRNA) and uterine inflammation in mares is unknown. We aimed (I) to investigate expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 (II) and to measure concentrations of interleukin 6 (IL-6), and prostaglandins (PGF<sub>2α</sub> and PGE<sub>2</sub>) in serum of mares with healthy and abnormal uterine status (endometritis). This study was conducted on 80 Arabian mares: young (4–7 years), and old (8–14 years). Mares were divided into 48 sub-fertile (endometritis) and 32 fertile (control) at stud farms. Serum was collected for measuring IL-6, PGF<sub>2α</sub>, and PGE<sub>2</sub>, as well as miRNA isolation and qRT-PCR. Concentrations of IL-6, PGE<sub>2</sub>, and PGF<sub>2α</sub> were higher in mares with endometritis compared to control. Age of mares had a remarkable effect on IL-6, PGE<sub>2</sub>, and PGF<sub>2α</sub> concentrations. Relative abundance of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 was higher in both young and old mares with endometritis. We noticed that eca-miR-155, eca-miR-223, eca-miR-200a, and eca-miR-205 revealed higher expression level in old than young mares with endometritis. This is the first study that has revealed the changes in cell free miRNA and serum inflammatory mediators during endometritis, and these findings could be used for a better understanding the pathophysiology mechanisms of endometritis in equine.https://www.mdpi.com/2306-7381/8/6/98serum miRNAmaresendometritisinterleukin 6prostaglandinsage |
| spellingShingle | Sally Ibrahim Mohamed Hedia Mohamed O. Taqi Mohamed K. Derbala Karima Gh. M. Mahmoud Youssef Ahmed Sayed Ismail Mohamed El-Belely Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares Veterinary Sciences serum miRNA mares endometritis interleukin 6 prostaglandins age |
| title | Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares |
| title_full | Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares |
| title_fullStr | Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares |
| title_full_unstemmed | Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares |
| title_short | Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares |
| title_sort | alterations in the expression profile of serum mir 155 mir 223 mir 17 mir 200a mir 205 as well as levels of interleukin 6 and prostaglandins during endometritis in arabian mares |
| topic | serum miRNA mares endometritis interleukin 6 prostaglandins age |
| url | https://www.mdpi.com/2306-7381/8/6/98 |
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