Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle
Skeletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | JACC: Basic to Translational Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452302X17302255 |
| _version_ | 1818000121663586304 |
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| author | Fiona C. Lewis, BSc, PhD Beverley J. Cottle, BSc, PhD Victoria Shone, BSc, MSc, PhD Giovanna Marazzi, MD David Sassoon, PhD Cheyenne C.S. Tseng, MD Patricia Y.W. Dankers, PhD Steven A.J. Chamuleau, MD, PhD Bernardo Nadal-Ginard, MD, PhD Georgina M. Ellison-Hughes, BSc, PhD |
| author_facet | Fiona C. Lewis, BSc, PhD Beverley J. Cottle, BSc, PhD Victoria Shone, BSc, MSc, PhD Giovanna Marazzi, MD David Sassoon, PhD Cheyenne C.S. Tseng, MD Patricia Y.W. Dankers, PhD Steven A.J. Chamuleau, MD, PhD Bernardo Nadal-Ginard, MD, PhD Georgina M. Ellison-Hughes, BSc, PhD |
| author_sort | Fiona C. Lewis, BSc, PhD |
| collection | DOAJ |
| description | Skeletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy. |
| first_indexed | 2024-04-14T03:18:46Z |
| format | Article |
| id | doaj.art-263f81d3ce8e4043b6a52ae6dc467ffe |
| institution | Directory Open Access Journal |
| issn | 2452-302X |
| language | English |
| last_indexed | 2024-04-14T03:18:46Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JACC: Basic to Translational Science |
| spelling | doaj.art-263f81d3ce8e4043b6a52ae6dc467ffe2022-12-22T02:15:23ZengElsevierJACC: Basic to Translational Science2452-302X2017-12-012671773610.1016/j.jacbts.2017.08.002Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal MuscleFiona C. Lewis, BSc, PhD0Beverley J. Cottle, BSc, PhD1Victoria Shone, BSc, MSc, PhD2Giovanna Marazzi, MD3David Sassoon, PhD4Cheyenne C.S. Tseng, MD5Patricia Y.W. Dankers, PhD6Steven A.J. Chamuleau, MD, PhD7Bernardo Nadal-Ginard, MD, PhD8Georgina M. Ellison-Hughes, BSc, PhD9School of Basic & Medical Biosciences, Centre of Human & Aerospace Physiological Sciences & Centre for Stem Cells and Regenerative Medicine, Faculty of Life Sciences & Medicine, King’s College London, Guy’s Campus, London, United KingdomSchool of Basic & Medical Biosciences, Centre of Human & Aerospace Physiological Sciences & Centre for Stem Cells and Regenerative Medicine, Faculty of Life Sciences & Medicine, King’s College London, Guy’s Campus, London, United KingdomSchool of Basic & Medical Biosciences, Centre of Human & Aerospace Physiological Sciences & Centre for Stem Cells and Regenerative Medicine, Faculty of Life Sciences & Medicine, King’s College London, Guy’s Campus, London, United KingdomStem Cells and Regenerative Medicine UMRS 1166, Institute of Cardiometabolism and Nutrition, Université de Pierre et Marie Curie, Sorbonne Universités, Paris, FranceStem Cells and Regenerative Medicine UMRS 1166, Institute of Cardiometabolism and Nutrition, Université de Pierre et Marie Curie, Sorbonne Universités, Paris, FranceDepartment of Cardiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, the NetherlandsSupramolecular Biomaterials for Translational Biomedical Science, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the NetherlandsDepartment of Cardiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, the NetherlandsSchool of Basic & Medical Biosciences, Centre of Human & Aerospace Physiological Sciences & Centre for Stem Cells and Regenerative Medicine, Faculty of Life Sciences & Medicine, King’s College London, Guy’s Campus, London, United KingdomSchool of Basic & Medical Biosciences, Centre of Human & Aerospace Physiological Sciences & Centre for Stem Cells and Regenerative Medicine, Faculty of Life Sciences & Medicine, King’s College London, Guy’s Campus, London, United KingdomSkeletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.http://www.sciencedirect.com/science/article/pii/S2452302X17302255allogeneic progenitor cellsgrowth factorsPICsporcine preclinical modelregenerationskeletal muscle |
| spellingShingle | Fiona C. Lewis, BSc, PhD Beverley J. Cottle, BSc, PhD Victoria Shone, BSc, MSc, PhD Giovanna Marazzi, MD David Sassoon, PhD Cheyenne C.S. Tseng, MD Patricia Y.W. Dankers, PhD Steven A.J. Chamuleau, MD, PhD Bernardo Nadal-Ginard, MD, PhD Georgina M. Ellison-Hughes, BSc, PhD Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle JACC: Basic to Translational Science allogeneic progenitor cells growth factors PICs porcine preclinical model regeneration skeletal muscle |
| title | Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle |
| title_full | Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle |
| title_fullStr | Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle |
| title_full_unstemmed | Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle |
| title_short | Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle |
| title_sort | transplantation of allogeneic pw1pos pax7neg interstitial cells enhance endogenous repair of injured porcine skeletal muscle |
| topic | allogeneic progenitor cells growth factors PICs porcine preclinical model regeneration skeletal muscle |
| url | http://www.sciencedirect.com/science/article/pii/S2452302X17302255 |
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