Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists
In plants there is no universal protocol for RNA extraction, since optimizations are required depending on the species, tissues and developmental stages. Some plants/tissues are rich in secondary metabolites or synthesize thick cell walls, which hinder an efficient RNA extraction. One such example i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-07-01
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| Series: | Fibers |
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| Online Access: | http://www.mdpi.com/2079-6439/4/3/23 |
| _version_ | 1798006575612297216 |
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| author | Gea Guerriero Lauralie Mangeot-Peter Jean-Francois Hausman Sylvain Legay |
| author_facet | Gea Guerriero Lauralie Mangeot-Peter Jean-Francois Hausman Sylvain Legay |
| author_sort | Gea Guerriero |
| collection | DOAJ |
| description | In plants there is no universal protocol for RNA extraction, since optimizations are required depending on the species, tissues and developmental stages. Some plants/tissues are rich in secondary metabolites or synthesize thick cell walls, which hinder an efficient RNA extraction. One such example is bast fibres, long extraxylary cells characterized by a thick cellulosic cell wall. Given the economic importance of bast fibres, which are used in the textile sector, as well as in biocomposites as green substitutes of glass fibres, it is desirable to better understand their development from a molecular point of view. This knowledge favours the development of biotechnological strategies aimed at improving specific properties of bast fibres. To be able to perform high-throughput analyses, such as, for instance, transcriptomics of bast fibres, RNA extraction is a crucial and limiting step. We here detail a protocol enabling the rapid extraction of high quality RNA from the bast fibres of textile hemp, Cannabis sativa L., a multi-purpose fibre crop standing in the spotlight of research. |
| first_indexed | 2024-04-11T12:56:53Z |
| format | Article |
| id | doaj.art-26414d0c79924f99ab34db35e5389f06 |
| institution | Directory Open Access Journal |
| issn | 2079-6439 |
| language | English |
| last_indexed | 2024-04-11T12:56:53Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fibers |
| spelling | doaj.art-26414d0c79924f99ab34db35e5389f062022-12-22T04:23:03ZengMDPI AGFibers2079-64392016-07-01432310.3390/fib4030023fib4030023Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular BiologistsGea Guerriero0Lauralie Mangeot-Peter1Jean-Francois Hausman2Sylvain Legay3Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgEnvironmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgEnvironmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgEnvironmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgIn plants there is no universal protocol for RNA extraction, since optimizations are required depending on the species, tissues and developmental stages. Some plants/tissues are rich in secondary metabolites or synthesize thick cell walls, which hinder an efficient RNA extraction. One such example is bast fibres, long extraxylary cells characterized by a thick cellulosic cell wall. Given the economic importance of bast fibres, which are used in the textile sector, as well as in biocomposites as green substitutes of glass fibres, it is desirable to better understand their development from a molecular point of view. This knowledge favours the development of biotechnological strategies aimed at improving specific properties of bast fibres. To be able to perform high-throughput analyses, such as, for instance, transcriptomics of bast fibres, RNA extraction is a crucial and limiting step. We here detail a protocol enabling the rapid extraction of high quality RNA from the bast fibres of textile hemp, Cannabis sativa L., a multi-purpose fibre crop standing in the spotlight of research.http://www.mdpi.com/2079-6439/4/3/23RNA extractionbast fibresCannabis sativaintegrity |
| spellingShingle | Gea Guerriero Lauralie Mangeot-Peter Jean-Francois Hausman Sylvain Legay Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists Fibers RNA extraction bast fibres Cannabis sativa integrity |
| title | Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists |
| title_full | Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists |
| title_fullStr | Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists |
| title_full_unstemmed | Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists |
| title_short | Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists |
| title_sort | extraction of high quality rna from cannabis sativa bast fibres a vademecum for molecular biologists |
| topic | RNA extraction bast fibres Cannabis sativa integrity |
| url | http://www.mdpi.com/2079-6439/4/3/23 |
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