tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight
Abstract Background Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating fungal disease of wheat. The mechanism underlying F. graminearum-wheat interaction remains largely unknown. tRNA-derived fragments (tRFs) are RNase-dependent small RNAs derived from tRNAs, and they have no...
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| Format: | Article |
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BMC
2022-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03393-9 |
| _version_ | 1819228962085142528 |
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| author | Zhengxi Sun Yi Hu Yilei Zhou Ning Jiang Sijia Hu Lei Li Tao Li |
| author_facet | Zhengxi Sun Yi Hu Yilei Zhou Ning Jiang Sijia Hu Lei Li Tao Li |
| author_sort | Zhengxi Sun |
| collection | DOAJ |
| description | Abstract Background Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating fungal disease of wheat. The mechanism underlying F. graminearum-wheat interaction remains largely unknown. tRNA-derived fragments (tRFs) are RNase-dependent small RNAs derived from tRNAs, and they have not been reported in wheat yet, and whether tRFs are involved in wheat-F. graminearum interactions remains unknown. Results Herein, small RNAs from the spikelets inoculated with F. graminearum and mock from an FHB-susceptible variety Chinese Spring (CS) and an FHB-resistant variety Sumai3 (SM) were sequenced respectively. A total of 1249 putative tRFs were identified, in which 15 tRFs was CS-specific and 12 SM-specific. Compared with mock inoculation, 39 tRFs were significantly up-regulated across both wheat varieties after F. graminearum challenge and only nine tRFs were significantly down-regulated. tRFGlu, tRFLys and tRFThr were dramatically induced by F. graminearum infection, with significantly higher fold changes in CS than those in SM. The expression patterns of the three highly induced tRFs were further validated by stem-loop qRT-PCR. The accumulation of tRFs were closely related to ribonucleases T2 family members, which were induced by F. graminearum challenge. The tRFs’ targets in host were predicted and were validated by RNA sequencing. Conclusion Integrative analysis of the differentially expressed tRFs and their candidate targets indicated that tRFGlu, tRFLys and tRFThr might negatively regulate wheat resistance to FHB. Our results unvealed the potential roles of tRFs in wheat-F. graminearum interactions. |
| first_indexed | 2024-12-23T11:05:36Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-23T11:05:36Z |
| publishDate | 2022-01-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-264571f9f7ab4c55b5d1b046c3bade112022-12-21T17:49:29ZengBMCBMC Plant Biology1471-22292022-01-0122111710.1186/s12870-021-03393-9tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blightZhengxi Sun0Yi Hu1Yilei Zhou2Ning Jiang3Sijia Hu4Lei Li5Tao Li6Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu, Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou UniversityKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu, Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou UniversityKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu, Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou UniversityKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu, Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou UniversityKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu, Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou UniversityKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu, Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou UniversityKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu, Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovation of Modern Crops and Food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou UniversityAbstract Background Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating fungal disease of wheat. The mechanism underlying F. graminearum-wheat interaction remains largely unknown. tRNA-derived fragments (tRFs) are RNase-dependent small RNAs derived from tRNAs, and they have not been reported in wheat yet, and whether tRFs are involved in wheat-F. graminearum interactions remains unknown. Results Herein, small RNAs from the spikelets inoculated with F. graminearum and mock from an FHB-susceptible variety Chinese Spring (CS) and an FHB-resistant variety Sumai3 (SM) were sequenced respectively. A total of 1249 putative tRFs were identified, in which 15 tRFs was CS-specific and 12 SM-specific. Compared with mock inoculation, 39 tRFs were significantly up-regulated across both wheat varieties after F. graminearum challenge and only nine tRFs were significantly down-regulated. tRFGlu, tRFLys and tRFThr were dramatically induced by F. graminearum infection, with significantly higher fold changes in CS than those in SM. The expression patterns of the three highly induced tRFs were further validated by stem-loop qRT-PCR. The accumulation of tRFs were closely related to ribonucleases T2 family members, which were induced by F. graminearum challenge. The tRFs’ targets in host were predicted and were validated by RNA sequencing. Conclusion Integrative analysis of the differentially expressed tRFs and their candidate targets indicated that tRFGlu, tRFLys and tRFThr might negatively regulate wheat resistance to FHB. Our results unvealed the potential roles of tRFs in wheat-F. graminearum interactions.https://doi.org/10.1186/s12870-021-03393-9Fusarium head blighttRNA-derived small RNA fragmentsRNase T2 |
| spellingShingle | Zhengxi Sun Yi Hu Yilei Zhou Ning Jiang Sijia Hu Lei Li Tao Li tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight BMC Plant Biology Fusarium head blight tRNA-derived small RNA fragments RNase T2 |
| title | tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight |
| title_full | tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight |
| title_fullStr | tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight |
| title_full_unstemmed | tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight |
| title_short | tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight |
| title_sort | trna derived fragments from wheat are potentially involved in susceptibility to fusarium head blight |
| topic | Fusarium head blight tRNA-derived small RNA fragments RNase T2 |
| url | https://doi.org/10.1186/s12870-021-03393-9 |
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