Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts
We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2020-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000004V.pdf |
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| author | Veličković Ivona Žižak Željko Rajčević Nemanja Ivanov Marija Soković Marina Marin Petar Grujić Slavica |
| author_facet | Veličković Ivona Žižak Željko Rajčević Nemanja Ivanov Marija Soković Marina Marin Petar Grujić Slavica |
| author_sort | Veličković Ivona |
| collection | DOAJ |
| description | We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173029, Grant no. 173032 and Grant no. 175011] |
| first_indexed | 2024-12-20T20:09:41Z |
| format | Article |
| id | doaj.art-264703389c8c4c69aa9467c10f0645fc |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-20T20:09:41Z |
| publishDate | 2020-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-264703389c8c4c69aa9467c10f0645fc2022-12-21T19:27:50ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392020-01-0172110511510.2298/ABS191217004V0354-46642000004VExamination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extractsVeličković Ivona0Žižak Željko1Rajčević Nemanja2Ivanov Marija3Soković Marina4Marin Petar5Grujić Slavica6University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Belgrade, SerbiaInstitute of Oncology and Radiology of Serbia, Belgrade, SerbiaUniversity of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Belgrade, SerbiaUniversity of Belgrade, Institute for Biological Research “Siniša Stanković“, National Institute of the Republic of Serbia, Belgrade, Belgrade, SerbiaUniversity of Belgrade, Institute for Biological Research “Siniša Stanković“, National Institute of the Republic of Serbia, Belgrade, Belgrade, SerbiaUniversity of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Belgrade, SerbiaUniversity of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Belgrade, SerbiaWe investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173029, Grant no. 173032 and Grant no. 175011]http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000004V.pdfp. spinosa (blackthorn)antioxidant activityantimicrobial activityantidiabetic activityα-glucosidase inhibitory activity |
| spellingShingle | Veličković Ivona Žižak Željko Rajčević Nemanja Ivanov Marija Soković Marina Marin Petar Grujić Slavica Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts Archives of Biological Sciences p. spinosa (blackthorn) antioxidant activity antimicrobial activity antidiabetic activity α-glucosidase inhibitory activity |
| title | Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts |
| title_full | Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts |
| title_fullStr | Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts |
| title_full_unstemmed | Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts |
| title_short | Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts |
| title_sort | examination of the polyphenol content and bioactivities of prunus spinosa l fruit extracts |
| topic | p. spinosa (blackthorn) antioxidant activity antimicrobial activity antidiabetic activity α-glucosidase inhibitory activity |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000004V.pdf |
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