Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles
Highly fluorescent magnetic nanoparticles (Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION) [europium (III) chloride hexahydrate = Eu; 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione = TTA; trioctylphosphine = (P(Oct)<sub>...
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MDPI AG
2020-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/7/1312 |
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| author | Myeong Yun Kim Jong-Pil Ahn Seung Yun Han Nam-Seob Lee Young Gil Jeong Do Kyung Kim |
| author_facet | Myeong Yun Kim Jong-Pil Ahn Seung Yun Han Nam-Seob Lee Young Gil Jeong Do Kyung Kim |
| author_sort | Myeong Yun Kim |
| collection | DOAJ |
| description | Highly fluorescent magnetic nanoparticles (Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION) [europium (III) chloride hexahydrate = Eu; 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione = TTA; trioctylphosphine = (P(Oct)<sub>3</sub>); mesoporous silica = mSiO<sub>2</sub>; superparamagnetic iron oxide nanoparticle = SPION] were developed as a dual-functional imaging agent. The hierarchical structure was composed of a magnetic core and mesoporous silica shell was constructed using a cationic surfactant template after coating with phosphatidylcholine of oleic acid coated SPION. Afterward, the surface and cavities of mSiO<sub>2</sub>@SPION were modified with 3-(trimethoxysilyl) propyl methacrylate (TMSPMA) as a silane coupling agent to introduce methacrylate groups. Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub> molecules are penetrated, located and bonded covalently inside of the cavities/mesopores of mSiO<sub>2</sub>, it shows extremely stable anti-photobleaching properties. The emission spectra of Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION indicated typical hypersensitivity transition <sup>5</sup>D<sub>0</sub>→<sup>7</sup>F<sub>2</sub> at 621 nm. The concentration of Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION was varied between 10 and 500 μL/mL to evaluate the cytotoxicity with NCI-H460 (H460) cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. In addition, the presence of a strong red-emitting Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION in the cytoplasm was observed by fluorescence microscopy. Those results that it can be a potential candidate for dual-functional contrast agent and PL nanomaterials for fabricating the diagnostic kits to amplify the low signal. |
| first_indexed | 2024-03-09T06:11:19Z |
| format | Article |
| id | doaj.art-26475d65c9154d758939445f17187e27 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T06:11:19Z |
| publishDate | 2020-07-01 |
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| series | Nanomaterials |
| spelling | doaj.art-26475d65c9154d758939445f17187e272023-12-03T11:58:17ZengMDPI AGNanomaterials2079-49912020-07-01107131210.3390/nano10071312Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide NanoparticlesMyeong Yun Kim0Jong-Pil Ahn1Seung Yun Han2Nam-Seob Lee3Young Gil Jeong4Do Kyung Kim5Department of Anatomy, College of Medicine, Konyang University Hospital, Daejeon 35365, KoreaDepartment of Business Cooperation Center, Korea Institute of Ceramic Engineering and Technology, Bucheon 14502, KoreaDepartment of Anatomy, College of Medicine, Konyang University Hospital, Daejeon 35365, KoreaDepartment of Anatomy, College of Medicine, Konyang University Hospital, Daejeon 35365, KoreaDepartment of Anatomy, College of Medicine, Konyang University Hospital, Daejeon 35365, KoreaDepartment of Anatomy, College of Medicine, Konyang University Hospital, Daejeon 35365, KoreaHighly fluorescent magnetic nanoparticles (Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION) [europium (III) chloride hexahydrate = Eu; 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione = TTA; trioctylphosphine = (P(Oct)<sub>3</sub>); mesoporous silica = mSiO<sub>2</sub>; superparamagnetic iron oxide nanoparticle = SPION] were developed as a dual-functional imaging agent. The hierarchical structure was composed of a magnetic core and mesoporous silica shell was constructed using a cationic surfactant template after coating with phosphatidylcholine of oleic acid coated SPION. Afterward, the surface and cavities of mSiO<sub>2</sub>@SPION were modified with 3-(trimethoxysilyl) propyl methacrylate (TMSPMA) as a silane coupling agent to introduce methacrylate groups. Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub> molecules are penetrated, located and bonded covalently inside of the cavities/mesopores of mSiO<sub>2</sub>, it shows extremely stable anti-photobleaching properties. The emission spectra of Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION indicated typical hypersensitivity transition <sup>5</sup>D<sub>0</sub>→<sup>7</sup>F<sub>2</sub> at 621 nm. The concentration of Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION was varied between 10 and 500 μL/mL to evaluate the cytotoxicity with NCI-H460 (H460) cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. In addition, the presence of a strong red-emitting Eu(TTA)<sub>3</sub>(P(Oct)<sub>3</sub>)<sub>3</sub>@mSiO<sub>2</sub>@SPION in the cytoplasm was observed by fluorescence microscopy. Those results that it can be a potential candidate for dual-functional contrast agent and PL nanomaterials for fabricating the diagnostic kits to amplify the low signal.https://www.mdpi.com/2079-4991/10/7/1312europium complexsuperparamagneticiron oxidemesoporous silicaphotoluminescent |
| spellingShingle | Myeong Yun Kim Jong-Pil Ahn Seung Yun Han Nam-Seob Lee Young Gil Jeong Do Kyung Kim Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles Nanomaterials europium complex superparamagnetic iron oxide mesoporous silica photoluminescent |
| title | Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles |
| title_full | Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles |
| title_fullStr | Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles |
| title_full_unstemmed | Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles |
| title_short | Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles |
| title_sort | highly luminescent and anti photobleaching core shell structure of mesoporous silica and phosphatidylcholine modified superparamagnetic iron oxide nanoparticles |
| topic | europium complex superparamagnetic iron oxide mesoporous silica photoluminescent |
| url | https://www.mdpi.com/2079-4991/10/7/1312 |
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