Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small Ruminants
Diarrhea is associated with gut microbiota, immunity, and metabolic alterations in goat kids and lambs. This study used 28 lambs (11 healthy and 17 diarrheic) and 20 goat kids (10 healthy and 10 diarrheic) to investigate the association between diarrhea occurrence and changes in gut microbiota, meta...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/14/11423 |
| _version_ | 1797589021488054272 |
|---|---|
| author | Xinlu Wang Lili Niu Yaxuan Wang Siyuan Zhan Linjie Wang Dinghui Dai Jiaxue Cao Jiazhong Guo Li Li Hongping Zhang Tao Zhong |
| author_facet | Xinlu Wang Lili Niu Yaxuan Wang Siyuan Zhan Linjie Wang Dinghui Dai Jiaxue Cao Jiazhong Guo Li Li Hongping Zhang Tao Zhong |
| author_sort | Xinlu Wang |
| collection | DOAJ |
| description | Diarrhea is associated with gut microbiota, immunity, and metabolic alterations in goat kids and lambs. This study used 28 lambs (11 healthy and 17 diarrheic) and 20 goat kids (10 healthy and 10 diarrheic) to investigate the association between diarrhea occurrence and changes in gut microbiota, metabolism, and immunity in goat kids and lambs. The results revealed that Firmicutes, Proteobacteria, and Bacteroidetes were the dominant phyla in goat kids and lambs. In addition, <i>Enterobacteriaceae</i> and <i>Lachnospiraceae</i> families were identified in both diarrheic goat kids and lambs. Furthermore, functional prediction of microbiota showed that it was involved in cell motility and cancer pathways. The identified differential metabolites were implicated in the bile secretion pathway. Lambs had significant differences in immunoglobulin G (IgG), immunoglobulin M (IgM), interleukin-1β (IL-1β), and tumor necrosis factor-alpha (TNF-α) compared to goat kids. IgG and IL-1β were positively correlated to Patescibacteria, <i>Clostridiaceae</i>, and unclassified_Muribaculaceae in both diarrheic goat kids and lambs. In addition, weighted gene co-expression network analysis (WGCNA) revealed that the MEgreen module was positively associated with IgG, IgM, IL-1β, TNF-α, and triglyceride (TG). In conclusion, our results characterized the gut microbiota, metabolism, and immune status of lambs and goat kids suffering from diarrhea. |
| first_indexed | 2024-03-11T01:00:23Z |
| format | Article |
| id | doaj.art-26491e3f36e84c79b2b6473670fa8ef5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:00:23Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-26491e3f36e84c79b2b6473670fa8ef52023-11-18T19:39:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141142310.3390/ijms241411423Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small RuminantsXinlu Wang0Lili Niu1Yaxuan Wang2Siyuan Zhan3Linjie Wang4Dinghui Dai5Jiaxue Cao6Jiazhong Guo7Li Li8Hongping Zhang9Tao Zhong10Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, ChinaDiarrhea is associated with gut microbiota, immunity, and metabolic alterations in goat kids and lambs. This study used 28 lambs (11 healthy and 17 diarrheic) and 20 goat kids (10 healthy and 10 diarrheic) to investigate the association between diarrhea occurrence and changes in gut microbiota, metabolism, and immunity in goat kids and lambs. The results revealed that Firmicutes, Proteobacteria, and Bacteroidetes were the dominant phyla in goat kids and lambs. In addition, <i>Enterobacteriaceae</i> and <i>Lachnospiraceae</i> families were identified in both diarrheic goat kids and lambs. Furthermore, functional prediction of microbiota showed that it was involved in cell motility and cancer pathways. The identified differential metabolites were implicated in the bile secretion pathway. Lambs had significant differences in immunoglobulin G (IgG), immunoglobulin M (IgM), interleukin-1β (IL-1β), and tumor necrosis factor-alpha (TNF-α) compared to goat kids. IgG and IL-1β were positively correlated to Patescibacteria, <i>Clostridiaceae</i>, and unclassified_Muribaculaceae in both diarrheic goat kids and lambs. In addition, weighted gene co-expression network analysis (WGCNA) revealed that the MEgreen module was positively associated with IgG, IgM, IL-1β, TNF-α, and triglyceride (TG). In conclusion, our results characterized the gut microbiota, metabolism, and immune status of lambs and goat kids suffering from diarrhea.https://www.mdpi.com/1422-0067/24/14/11423ruminantsimmunemetabolomeserum biochemistry16S rRNA |
| spellingShingle | Xinlu Wang Lili Niu Yaxuan Wang Siyuan Zhan Linjie Wang Dinghui Dai Jiaxue Cao Jiazhong Guo Li Li Hongping Zhang Tao Zhong Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small Ruminants International Journal of Molecular Sciences ruminants immune metabolome serum biochemistry 16S rRNA |
| title | Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small Ruminants |
| title_full | Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small Ruminants |
| title_fullStr | Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small Ruminants |
| title_full_unstemmed | Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small Ruminants |
| title_short | Combining 16S rRNA Sequencing and Metabolomics Data to Decipher the Interactions between Gut Microbiota, Host Immunity, and Metabolites in Diarrheic Young Small Ruminants |
| title_sort | combining 16s rrna sequencing and metabolomics data to decipher the interactions between gut microbiota host immunity and metabolites in diarrheic young small ruminants |
| topic | ruminants immune metabolome serum biochemistry 16S rRNA |
| url | https://www.mdpi.com/1422-0067/24/14/11423 |
| work_keys_str_mv | AT xinluwang combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT liliniu combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT yaxuanwang combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT siyuanzhan combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT linjiewang combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT dinghuidai combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT jiaxuecao combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT jiazhongguo combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT lili combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT hongpingzhang combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants AT taozhong combining16srrnasequencingandmetabolomicsdatatodeciphertheinteractionsbetweengutmicrobiotahostimmunityandmetabolitesindiarrheicyoungsmallruminants |