Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in China
Abstract Background Wildlife zoos provide the opportunity for children and adults to interact with animals, However, it's unknown that the risk of contact with animals, which carried zoonotic pathogens and antimicrobial resistant bacteria. Objectives This study aimed to investigate the prevalen...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.773 |
| _version_ | 1797683395317202944 |
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| author | Ziyue Zeng Jie Yang Jinrong Gu Zhihong Liu Jufang Hu Xiangyong Li Xiaojun Chen Zhiliang Sun Jiyun Li |
| author_facet | Ziyue Zeng Jie Yang Jinrong Gu Zhihong Liu Jufang Hu Xiangyong Li Xiaojun Chen Zhiliang Sun Jiyun Li |
| author_sort | Ziyue Zeng |
| collection | DOAJ |
| description | Abstract Background Wildlife zoos provide the opportunity for children and adults to interact with animals, However, it's unknown that the risk of contact with animals, which carried zoonotic pathogens and antimicrobial resistant bacteria. Objectives This study aimed to investigate the prevalence and antimicrobial susceptibility of extended‐spectrum β‐lactamases Escherichia coli (ESBLs‐EC) from a wildlife zoo in China. Methods A total of 93 wildlife faecal samples were collected from a wildlife zoo. Agar dilution method was used to determine the resistant phenotype. Whole genomes sequencing and bioinformatic analysis were employed to evaluate the molecular typing and genetic relationships of ESBLs‐EC. Results A total of 23 CTX‐M‐positive ESBLs‐EC were isolated from swan (n = 14), squirrel monkey (n = 5), black hat hanging monkey (n = 2), gibbon monkey (n = 1) and phoenicopteridae (n = 1) respectively. All ESBLs‐EC strains were resistant to cefotaxime, tetracycline, ciprofloxacin and trimethoprim‐sulfamethoxazole, but susceptible to colistin, tigecycline, meropenem and amikacin. By screening whole genome sequences, ESBLs‐EC strains main carried blaCTX‐M‐55 (34.8%, 8/23) and blaCTX‐M‐14 (26.0%, 6/23), following by blaCTX‐M‐27 (21.7%, 5/23), blaCTX‐M‐15 (13.0%, 3/23) and blaCTX‐M‐121 (4.3%, 1/23). ESBLs‐EC strains mainly belonged to phylogroup A (60.9%, 14/23), and ST48, ST746 and ST616 (3 strains respectively, 13.0%) were major ST types. Core genome‐based single nucleotide polymorphism (SNP) analysis suggested that strains from the swan, over the phylogenetic tree, have a closer genetic relationship with strains from other animals (black hat hanging monkey, gibbon monkey, phoenicopteridae and squirrel monkey). Conclusions CTX‐M type ESBLs‐EC can transmit between animals in wildlife zoos, which may be a risk of spread to animal keepers, veterinarians and visitors when contact with animals. Our study provides that the importance of hygiene measures to minimise the risk of transmission of ESBLs‐EC to visitors in wildlife zoos. |
| first_indexed | 2024-03-12T00:14:43Z |
| format | Article |
| id | doaj.art-264aa9940d4f4d5eaa252801a8277247 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-12T00:14:43Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-264aa9940d4f4d5eaa252801a82772472023-09-15T15:20:44ZengWileyVeterinary Medicine and Science2053-10952022-05-01831294129910.1002/vms3.773Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in ChinaZiyue Zeng0Jie Yang1Jinrong Gu2Zhihong Liu3Jufang Hu4Xiangyong Li5Xiaojun Chen6Zhiliang Sun7Jiyun Li8College of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaChangsha Ecological Zoo Changsha Hunan ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaAbstract Background Wildlife zoos provide the opportunity for children and adults to interact with animals, However, it's unknown that the risk of contact with animals, which carried zoonotic pathogens and antimicrobial resistant bacteria. Objectives This study aimed to investigate the prevalence and antimicrobial susceptibility of extended‐spectrum β‐lactamases Escherichia coli (ESBLs‐EC) from a wildlife zoo in China. Methods A total of 93 wildlife faecal samples were collected from a wildlife zoo. Agar dilution method was used to determine the resistant phenotype. Whole genomes sequencing and bioinformatic analysis were employed to evaluate the molecular typing and genetic relationships of ESBLs‐EC. Results A total of 23 CTX‐M‐positive ESBLs‐EC were isolated from swan (n = 14), squirrel monkey (n = 5), black hat hanging monkey (n = 2), gibbon monkey (n = 1) and phoenicopteridae (n = 1) respectively. All ESBLs‐EC strains were resistant to cefotaxime, tetracycline, ciprofloxacin and trimethoprim‐sulfamethoxazole, but susceptible to colistin, tigecycline, meropenem and amikacin. By screening whole genome sequences, ESBLs‐EC strains main carried blaCTX‐M‐55 (34.8%, 8/23) and blaCTX‐M‐14 (26.0%, 6/23), following by blaCTX‐M‐27 (21.7%, 5/23), blaCTX‐M‐15 (13.0%, 3/23) and blaCTX‐M‐121 (4.3%, 1/23). ESBLs‐EC strains mainly belonged to phylogroup A (60.9%, 14/23), and ST48, ST746 and ST616 (3 strains respectively, 13.0%) were major ST types. Core genome‐based single nucleotide polymorphism (SNP) analysis suggested that strains from the swan, over the phylogenetic tree, have a closer genetic relationship with strains from other animals (black hat hanging monkey, gibbon monkey, phoenicopteridae and squirrel monkey). Conclusions CTX‐M type ESBLs‐EC can transmit between animals in wildlife zoos, which may be a risk of spread to animal keepers, veterinarians and visitors when contact with animals. Our study provides that the importance of hygiene measures to minimise the risk of transmission of ESBLs‐EC to visitors in wildlife zoos.https://doi.org/10.1002/vms3.773CTX‐ME. coliESBLswildlifezoo |
| spellingShingle | Ziyue Zeng Jie Yang Jinrong Gu Zhihong Liu Jufang Hu Xiangyong Li Xiaojun Chen Zhiliang Sun Jiyun Li Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in China Veterinary Medicine and Science CTX‐M E. coli ESBLs wildlife zoo |
| title | Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in China |
| title_full | Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in China |
| title_fullStr | Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in China |
| title_full_unstemmed | Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in China |
| title_short | Prevalence and antimicrobial susceptibility of CTX‐M‐type‐producing Escherichia coli from a wildlife zoo in China |
| title_sort | prevalence and antimicrobial susceptibility of ctx m type producing escherichia coli from a wildlife zoo in china |
| topic | CTX‐M E. coli ESBLs wildlife zoo |
| url | https://doi.org/10.1002/vms3.773 |
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