The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro
Background: <i>Sternbergia clusiana</i> belongs to the Amaryllidaceae family and is recognized for the valuable biological activity of its major bioactive compounds. The aim of the current is to evaluate the anticancer effects of the ethanolic bulb extract of <i>Sternbergia clusian...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/3/529 |
| _version_ | 1797623450522615808 |
|---|---|
| author | Mona El Samarji Maria Younes Marianne El Khoury Tony Haykal Nazira Elias Natalia Gasilova Laure Menin Ahmad Houri Nisrine Machaka-Houri Sandra Rizk |
| author_facet | Mona El Samarji Maria Younes Marianne El Khoury Tony Haykal Nazira Elias Natalia Gasilova Laure Menin Ahmad Houri Nisrine Machaka-Houri Sandra Rizk |
| author_sort | Mona El Samarji |
| collection | DOAJ |
| description | Background: <i>Sternbergia clusiana</i> belongs to the Amaryllidaceae family and is recognized for the valuable biological activity of its major bioactive compounds. The aim of the current is to evaluate the anticancer effects of the ethanolic bulb extract of <i>Sternbergia clusiana</i> (ScBEE) on breast cancer cells in vitro and to further reveal the underlying cellular mechanism. Methods: An MTS cell viability assay was performed on MDA-MB-231 and MCF-7 cells, along with cell cycle analysis, cell death ELISA, Western blot analysis and an ROS production assay to decipher the mechanism of death. LC-MS/MS was also performed to identify the chemical composition of this ethanolic extract. Results: The results show a selective antiproliferative effect on both cell lines with no effect on normal mesenchymal stem cells. Further analysis suggested the activation of the apoptotic pathway as reflected by the increase in cellular and DNA fragmentation and alterations in apoptotic proteins such as Bax, Bcl-2 and c-PARP. ScBEE was also found to exhibit antioxidant effect, as shown by a decrease in ROS production. The underlying mechanism of action was explained by the presence of several bioactive compounds identified by LC-MS/MS, including alkaloids, terpenoids and phenols, which are elaborated in the manuscript. Conclusion: This study highlights the antioxidant and anticancerous properties of <i>S.clusiana</i> for breast cancer treatment. |
| first_indexed | 2024-03-11T09:29:10Z |
| format | Article |
| id | doaj.art-264b7721e0f84ac18de76ccc9d04294c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:10Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-264b7721e0f84ac18de76ccc9d04294c2023-11-16T17:43:41ZengMDPI AGPlants2223-77472023-01-0112352910.3390/plants12030529The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In VitroMona El Samarji0Maria Younes1Marianne El Khoury2Tony Haykal3Nazira Elias4Natalia Gasilova5Laure Menin6Ahmad Houri7Nisrine Machaka-Houri8Sandra Rizk9Department of Natural Sciences, Lebanese American University, Byblos P.O. Box 36, LebanonDepartment of Natural Sciences, Lebanese American University, Byblos P.O. Box 36, LebanonDepartment of Natural Sciences, Lebanese American University, Byblos P.O. Box 36, LebanonDepartment of Natural Sciences, Lebanese American University, Byblos P.O. Box 36, LebanonDepartment of Natural Sciences, Lebanese American University, Byblos P.O. Box 36, LebanonInstitute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, SwitzerlandInstitute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, SwitzerlandDepartment of Natural Sciences, Lebanese American University, Beirut 1102-2801, LebanonDepartment of Life and Earth Science, Faculty of Sciences, Saint Joseph University, Ras Maska 1104-2020, LebanonDepartment of Natural Sciences, Lebanese American University, Byblos P.O. Box 36, LebanonBackground: <i>Sternbergia clusiana</i> belongs to the Amaryllidaceae family and is recognized for the valuable biological activity of its major bioactive compounds. The aim of the current is to evaluate the anticancer effects of the ethanolic bulb extract of <i>Sternbergia clusiana</i> (ScBEE) on breast cancer cells in vitro and to further reveal the underlying cellular mechanism. Methods: An MTS cell viability assay was performed on MDA-MB-231 and MCF-7 cells, along with cell cycle analysis, cell death ELISA, Western blot analysis and an ROS production assay to decipher the mechanism of death. LC-MS/MS was also performed to identify the chemical composition of this ethanolic extract. Results: The results show a selective antiproliferative effect on both cell lines with no effect on normal mesenchymal stem cells. Further analysis suggested the activation of the apoptotic pathway as reflected by the increase in cellular and DNA fragmentation and alterations in apoptotic proteins such as Bax, Bcl-2 and c-PARP. ScBEE was also found to exhibit antioxidant effect, as shown by a decrease in ROS production. The underlying mechanism of action was explained by the presence of several bioactive compounds identified by LC-MS/MS, including alkaloids, terpenoids and phenols, which are elaborated in the manuscript. Conclusion: This study highlights the antioxidant and anticancerous properties of <i>S.clusiana</i> for breast cancer treatment.https://www.mdpi.com/2223-7747/12/3/529breast cancer<i>Sternbergia clusiana</i>phytochemicalsapoptosisantioxidant |
| spellingShingle | Mona El Samarji Maria Younes Marianne El Khoury Tony Haykal Nazira Elias Natalia Gasilova Laure Menin Ahmad Houri Nisrine Machaka-Houri Sandra Rizk The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro Plants breast cancer <i>Sternbergia clusiana</i> phytochemicals apoptosis antioxidant |
| title | The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro |
| title_full | The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro |
| title_fullStr | The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro |
| title_full_unstemmed | The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro |
| title_short | The Antioxidant and Proapoptotic Effects of <i>Sternbergia clusiana</i> Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro |
| title_sort | antioxidant and proapoptotic effects of i sternbergia clusiana i bulb ethanolic extract on triple negative and estrogen dependent breast cancer cells in vitro |
| topic | breast cancer <i>Sternbergia clusiana</i> phytochemicals apoptosis antioxidant |
| url | https://www.mdpi.com/2223-7747/12/3/529 |
| work_keys_str_mv | AT monaelsamarji theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT mariayounes theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT marianneelkhoury theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT tonyhaykal theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT naziraelias theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT nataliagasilova theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT lauremenin theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT ahmadhouri theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT nisrinemachakahouri theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT sandrarizk theantioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT monaelsamarji antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT mariayounes antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT marianneelkhoury antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT tonyhaykal antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT naziraelias antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT nataliagasilova antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT lauremenin antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT ahmadhouri antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT nisrinemachakahouri antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro AT sandrarizk antioxidantandproapoptoticeffectsofisternbergiaclusianaibulbethanolicextractontriplenegativeandestrogendependentbreastcancercellsinvitro |