Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer
Purpose: To investigate a potential alteration in the expression levels of transforminggrowth factor β (TGF-β) and miR-21 in bladder cancer tissues.Material and Methods: Using real-time polymerase chain reaction (PCR) method, weexamined a potential correlated expression of miR-21 and TGF-β variants...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences
2013-09-01
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| Series: | Urology Journal |
| Subjects: | |
| Online Access: | http://www.urologyjournal.org/index.php/uj/article/view/1487/752 |
| _version_ | 1818340039372832768 |
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| author | Hamideh Monfared Seyed Amir Mohsen Ziaee Mahmoud Hashemitabar Hamid Khayatzadeh Vahid Kheyrollahi Mahmood Tavallaei Seyed Javad Mowla |
| author_facet | Hamideh Monfared Seyed Amir Mohsen Ziaee Mahmoud Hashemitabar Hamid Khayatzadeh Vahid Kheyrollahi Mahmood Tavallaei Seyed Javad Mowla |
| author_sort | Hamideh Monfared |
| collection | DOAJ |
| description | Purpose: To investigate a potential alteration in the expression levels of transforminggrowth factor β (TGF-β) and miR-21 in bladder cancer tissues.Material and Methods: Using real-time polymerase chain reaction (PCR) method, weexamined a potential correlated expression of miR-21 and TGF-β variants in 30 bladdertumors and their marginal/non-tumor biopsy specimens obtained from the same patients.Results: Our data revealed a significant down-regulation of TGF-β variants (P = .03) alongwith a non-significant alteration in the expression of miR-21 in tumor vs. non-tumor samples.However, in contrast to low-grade tumors, the expression of miR-21 was upregulatedin high-grade ones, and the expression level can efficiently discriminate low-grade tumorsfrom high-grade ones (P = .03).Conclusion: In accordance to the observed similarity between TGF-β variants and miR-21gene expression alterations in bladder tumors, treating 5637 bladder cancer cell line withTGF-β recombinant protein caused a significant upregulation of miR-21. The later findingfurther confirmed a correlated expression of TGF-β and miR-21 in bladder tumors. |
| first_indexed | 2024-12-13T15:36:34Z |
| format | Article |
| id | doaj.art-264babbdb86a4f7684888de9f052b25a |
| institution | Directory Open Access Journal |
| issn | 1735-1308 1735-546X |
| language | English |
| last_indexed | 2024-12-13T15:36:34Z |
| publishDate | 2013-09-01 |
| publisher | Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Urology Journal |
| spelling | doaj.art-264babbdb86a4f7684888de9f052b25a2022-12-21T23:40:01ZengUrology and Nephrology Research Center, Shahid Beheshti University of Medical SciencesUrology Journal1735-13081735-546X2013-09-01103981987Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder CancerHamideh MonfaredSeyed Amir Mohsen ZiaeeMahmoud HashemitabarHamid KhayatzadehVahid KheyrollahiMahmood TavallaeiSeyed Javad MowlaPurpose: To investigate a potential alteration in the expression levels of transforminggrowth factor β (TGF-β) and miR-21 in bladder cancer tissues.Material and Methods: Using real-time polymerase chain reaction (PCR) method, weexamined a potential correlated expression of miR-21 and TGF-β variants in 30 bladdertumors and their marginal/non-tumor biopsy specimens obtained from the same patients.Results: Our data revealed a significant down-regulation of TGF-β variants (P = .03) alongwith a non-significant alteration in the expression of miR-21 in tumor vs. non-tumor samples.However, in contrast to low-grade tumors, the expression of miR-21 was upregulatedin high-grade ones, and the expression level can efficiently discriminate low-grade tumorsfrom high-grade ones (P = .03).Conclusion: In accordance to the observed similarity between TGF-β variants and miR-21gene expression alterations in bladder tumors, treating 5637 bladder cancer cell line withTGF-β recombinant protein caused a significant upregulation of miR-21. The later findingfurther confirmed a correlated expression of TGF-β and miR-21 in bladder tumors.http://www.urologyjournal.org/index.php/uj/article/view/1487/752biological markersanalysisurinary bladder neoplasmsmiR-21TGF-βgene expression profiling |
| spellingShingle | Hamideh Monfared Seyed Amir Mohsen Ziaee Mahmoud Hashemitabar Hamid Khayatzadeh Vahid Kheyrollahi Mahmood Tavallaei Seyed Javad Mowla Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer Urology Journal biological markers analysis urinary bladder neoplasms miR-21 TGF-β gene expression profiling |
| title | Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer |
| title_full | Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer |
| title_fullStr | Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer |
| title_full_unstemmed | Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer |
| title_short | Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer |
| title_sort | co regulated expression of tgf β variants and mir 21 in bladder cancer |
| topic | biological markers analysis urinary bladder neoplasms miR-21 TGF-β gene expression profiling |
| url | http://www.urologyjournal.org/index.php/uj/article/view/1487/752 |
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